Strain Name:
C57BL/6J-MtgxR2308Btlr/Mmmh
Stock Number:
040307-MU
Citation ID:
RRID:MMRRC_040307-MU
Other Names:
R2308 (G1), C57BL/6J-MtgxR2308Btlr
Major Collection:

Gene Information

Met
Name: met proto-oncogene
Synonyms: HGF receptor, c-Met, Par4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Epha7
Name: Eph receptor A7
Synonyms: MDK1, Ebk, Cek11, Ehk3, Hek11, Mdk1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13841
HGNC: HGNC:3390
Homologene: 20935
Mark2
Name: MAP/microtubule affinity regulating kinase 2
Synonyms: Par-1, Emk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 13728
HGNC: HGNC:3332
Homologene: 69013
Dot1l
Name: DOT1-like, histone H3 methyltransferase (S. cerevisiae)
Synonyms: mDot1, KMT4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 208266
Homologene: 32779
Rpap1
Name: RNA polymerase II associated protein 1
Synonyms: 1190005L06Rik, A730023M06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68925
Homologene: 32269
Ercc6
Name: excision repair cross-complementing rodent repair deficiency, complementation group 6
Synonyms: CS group B correcting gene, CSB, C130058G22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 319955
VEGA: 14
HGNC: HGNC:3438
Homologene: 133552
Txnl1
Name: thioredoxin-like 1
Synonyms: TRP32, 32kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 53382
VEGA: 18
Homologene: 3515
Exoc4
Name: exocyst complex component 4
Synonyms: Sec8, Sec8l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20336
Homologene: 40654
Depdc1b
Name: DEP domain containing 1B
Synonyms: XTP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218581
Homologene: 10157
Mbd1
Name: methyl-CpG binding domain protein 1
Synonyms: PCM1, Cxxc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 17190
VEGA: 18
HGNC: HGNC:6916
Homologene: 8414
Fntb
Name: farnesyltransferase, CAAX box, beta
Synonyms: 2010013E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 110606
HGNC: HGNC:3785
Homologene: 1535
Mcm2
Name: minichromosome maintenance complex component 2
Synonyms: CDCL1, BM28, Mcmd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17216
HGNC: HGNC:6944
Homologene: 3325
Slc41a3
Name: solute carrier family 41, member 3
Synonyms: SLC41A1-L2, 1010001P06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 71699
Homologene: 23052
Vps13d
Name: vacuolar protein sorting 13D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230895
Homologene: 15583
Ythdc2
Name: YTH domain containing 2
Synonyms: 3010002F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240255
Homologene: 11265
Unc80
Name: unc-80, NALCN activator
Synonyms: C230061B10Rik, C030018G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329178
Homologene: 122243
Cntnap4
Name: contactin associated protein-like 4
Synonyms: Caspr4, E130114F09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 170571
Homologene: 24912
Ppfia4
Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4
Synonyms: 1110008G13Rik, LOC100042382, Liprin-alpha4, Gm3812
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 68507
HGNC: HGNC:9248
Homologene: 66200
Zfp352
Name: zinc finger protein 352
Synonyms: 2czf48
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 236537
Homologene: 45160
Rbbp8
Name: retinoblastoma binding protein 8, endonuclease
Synonyms: 9930104E21Rik, CtIP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225182
VEGA: 18
HGNC: HGNC:9891
Homologene: 28546
Stkld1
Name: serine/threonine kinase-like domain containing 1
Synonyms: LOC279029, Gm711
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 279029
Homologene: 19586
Vmn2r80
Name: vomeronasal 2, receptor 80
Synonyms: EG624765
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 624765
Homologene: 83483
Nup210
Name: nucleoporin 210
Synonyms: gp210, gp190, Pom210
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54563
Homologene: 41286
Olfr356
Name: olfactory receptor 356
Synonyms: GA_x6K02T2NLDC-33631647-33632594, MOR134-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258617
Homologene: 85948
Gramd1a
Name: GRAM domain containing 1A
Synonyms: 1300003M23Rik, D7Bwg0611e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 52857
Homologene: 10843
Tbxas1
Name: thromboxane A synthase 1, platelet
Synonyms: TXS, CYP5A1, CYP5, TXAS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 21391
Homologene: 130979
Vmn2r118
Name: vomeronasal 2, receptor 118
Synonyms: EG668547, Vmn2r119, EG383258
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 383258
Homologene: 129687
Olfr1197
Name: olfactory receptor 1197
Synonyms: GA_x6K02T2Q125-50202854-50201910, MOR225-14, MOR225-10P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 433449
Homologene: 123770
Spaca7
Name: sperm acrosome associated 7
Synonyms: 1700094C09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 78634
Homologene: 49754
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 66,648,997 bp
  • A to G, chromosome 1 at 134,332,397 bp
  • A to T, chromosome 2 at 26,952,714 bp
  • T to A, chromosome 2 at 36,937,300 bp
  • T to A, chromosome 2 at 88,729,084 bp
  • G to A, chromosome 2 at 119,783,766 bp
  • G to A, chromosome 4 at 28,821,503 bp
  • A to G, chromosome 4 at 90,225,243 bp
  • T to C, chromosome 4 at 145,074,825 bp
  • T to C, chromosome 6 at 17,491,742 bp
  • A to G, chromosome 6 at 33,918,568 bp
  • A to G, chromosome 6 at 39,027,661 bp
  • G to A, chromosome 6 at 88,893,008 bp
  • T to A, chromosome 6 at 90,612,120 bp
  • T to C, chromosome 6 at 91,040,868 bp
  • T to C, chromosome 7 at 31,139,790 bp
  • A to T, chromosome 8 at 12,598,959 bp
  • C to T, chromosome 8 at 112,803,046 bp
  • T to C, chromosome 10 at 79,171,621 bp
  • T to C, chromosome 10 at 80,789,069 bp
  • C to A, chromosome 12 at 76,887,888 bp
  • G to A, chromosome 13 at 108,373,841 bp
  • T to C, chromosome 14 at 32,566,409 bp
  • A to G, chromosome 17 at 55,624,650 bp
  • A to T, chromosome 18 at 11,696,776 bp
  • A to G, chromosome 18 at 44,847,748 bp
  • T to C, chromosome 18 at 63,671,620 bp
  • A to G, chromosome 18 at 74,276,477 bp
  • A to C, chromosome 19 at 7,281,934 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2308 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040307-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.