Strain Name:
C57BL/6J-MtgxR2317Btlr/Mmmh
Stock Number:
040312-MU
Citation ID:
RRID:MMRRC_040312-MU
Other Names:
R2317 (G1), C57BL/6J-MtgxR2317Btlr
Major Collection:

Gene Information

Gabra6
Name: gamma-aminobutyric acid (GABA) A receptor, subunit alpha 6
Synonyms: Gabra-6, GABA-ARalpha6, alpha6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14399
HGNC: HGNC:4080
Homologene: 20220
Pnmt
Name: phenylethanolamine-N-methyltransferase
Synonyms: Pent
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18948
HGNC: HGNC:9160
Homologene: 55673
Zfp35
Name: zinc finger protein 35
Synonyms: Zfp-35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 22694
Homologene: 133081
Klf12
Name: Kruppel-like factor 12
Synonyms: D530033K05Rik, AP-2rep, 2700063E05Rik, B130052C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 16597
VEGA: 14
HGNC: HGNC:6346
Homologene: 21417
Pdcd6ip
Name: programmed cell death 6 interacting protein
Synonyms: Alix, AIP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18571
HGNC: HGNC:8766
Homologene: 22614
Nodal
Name: nodal
Synonyms: Tg.413d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18119
HGNC: HGNC:7865
Homologene: 8417
Neurod6
Name: neurogenic differentiation 6
Synonyms: Nex, Atoh2, bHLHa2, Math-2, Math2, Nex1m
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 11922
Homologene: 7632
Cwf19l1
Name: CWF19-like 1, cell cycle control (S. pombe)
Synonyms: 2610528C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 72502
VEGA: 19
Homologene: 41244
Slc25a36
Name: solute carrier family 25, member 36
Synonyms: C330005L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 192287
Homologene: 10039
Slc35e3
Name: solute carrier family 35, member E3
Synonyms: 9330166G04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215436
VEGA: 10
Homologene: 41279
Cript
Name: cysteine-rich PDZ-binding protein
Synonyms: 1200020A08Rik, CRIPT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 56724
Homologene: 8572
Ncoa1
Name: nuclear receptor coactivator 1
Synonyms: bHLHe74, SRC1, KAT13A, SRC-1, SRC-a/NCoA-1, steroid receptor coactivator-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 17977
VEGA: 12
HGNC: HGNC:7668
Homologene: 7859
Unc13b
Name: unc-13 homolog B
Synonyms: Munc13-2, Unc13h2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22249
Homologene: 31376
Cfap126
Name: cilia and flagella associated protein 126
Synonyms: Fltp, Flattop, 1700009P17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 75472
Homologene: 23682
Fbxo30
Name: F-box protein 30
Synonyms: 1700026A16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 71865
Homologene: 12959
Abca15
Name: ATP-binding cassette, sub-family A (ABC1), member 15
Synonyms: 4930500I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 320631
Homologene: 87255
Nuggc
Name: nuclear GTPase, germinal center associated
Synonyms: Gm600, SLIP-GC, LOC239151
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 100503545
Homologene: 72641
Gp1ba
Name: glycoprotein 1b, alpha polypeptide
Synonyms: GPIba, GPIb-alpha, GP Ib-alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14723
HGNC: HGNC:4439
Homologene: 143
Myrfl
Name: myelin regulatory factor-like
Synonyms: Gm239, LOC237558
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237558
VEGA: 10
Homologene: 88588
Zfp959
Name: zinc finger protein 959
Synonyms: BC011426
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224893
Homologene: 138295
Lats1
Name: large tumor suppressor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16798
VEGA: 10
HGNC: HGNC:6514
Homologene: 55843
Cdh15
Name: cadherin 15
Synonyms: Mcad, Cdh14, M cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12555
HGNC: HGNC:1754
Homologene: 3622
Stt3a
Name: STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
Synonyms: Itm1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 16430
HGNC: HGNC:6172
Homologene: 40617
Eif2b4
Name: eukaryotic translation initiation factor 2B, subunit 4 delta
Synonyms: Eif2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13667
HGNC: HGNC:3260
Homologene: 5976
Ccdc102a
Name: coiled-coil domain containing 102A
Synonyms: LOC234582
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234582
Homologene: 13240
Tedc2
Name: tubulin epsilon and delta complex 2
Synonyms: 1600002H07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72016
Homologene: 45943
Sprr2f
Name: small proline-rich protein 2F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20760
Esp16
Name: exocrine gland secreted peptide 16
Synonyms: Gm4345
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 100043300
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 171,126,131 bp
  • C to A, chromosome 3 at 92,366,083 bp
  • A to G, chromosome 4 at 43,245,514 bp
  • T to C, chromosome 5 at 31,191,576 bp
  • A to G, chromosome 6 at 55,678,921 bp
  • T to C, chromosome 7 at 120,373,689 bp
  • T to C, chromosome 8 at 94,908,329 bp
  • G to A, chromosome 8 at 122,856,635 bp
  • T to C, chromosome 9 at 36,748,075 bp
  • G to A, chromosome 9 at 97,079,182 bp
  • T to C, chromosome 9 at 113,672,774 bp
  • C to T, chromosome 10 at 7,691,776 bp
  • A to G, chromosome 10 at 11,290,334 bp
  • T to C, chromosome 10 at 61,418,433 bp
  • A to G, chromosome 10 at 116,839,384 bp
  • A to G, chromosome 10 at 117,744,899 bp
  • T to C, chromosome 11 at 42,317,780 bp
  • A to G, chromosome 11 at 70,640,647 bp
  • C to A, chromosome 11 at 98,386,851 bp
  • A to G, chromosome 12 at 4,275,189 bp
  • A to G, chromosome 14 at 65,624,142 bp
  • C to T, chromosome 14 at 99,942,067 bp
  • A to G, chromosome 17 at 24,216,384 bp
  • A to G, chromosome 17 at 39,539,847 bp
  • A to G, chromosome 17 at 55,897,326 bp
  • T to C, chromosome 17 at 87,027,711 bp
  • T to C, chromosome 18 at 24,003,498 bp
  • A to C, chromosome 19 at 44,132,158 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2317 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040312-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.