Strain Name:
C57BL/6J-MtgxR2346Btlr/Mmmh
Stock Number:
040329-MU
Citation ID:
RRID:MMRRC_040329-MU
Other Names:
R2346 (G1), C57BL/6J-MtgxR2346Btlr
Major Collection:

Gene Information

Prkar2b
Name: protein kinase, cAMP dependent regulatory, type II beta
Synonyms: RII(beta), PKARIIbeta, Pkarb2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 19088
HGNC: HGNC:9392
Homologene: 37666
Dnm2
Name: dynamin 2
Synonyms: Dyn2, b2b2159Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 13430
HGNC: HGNC:2974
Homologene: 90883
Kat2b
Name: K(lysine) acetyltransferase 2B
Synonyms: A930006P13Rik, Pcaf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18519
HGNC: HGNC:8638
Homologene: 20834
Shroom3
Name: shroom family member 3
Synonyms: Shrm, Shrm3, D5Ertd287e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 27428
Homologene: 9263
Nsf
Name: N-ethylmaleimide sensitive fusion protein
Synonyms: N-ethylmaleimide sensitive factor, SKD2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18195
HGNC: HGNC:8016
Homologene: 4502
Nfix
Name: nuclear factor I/X
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18032
HGNC: HGNC:7788
Homologene: 1872
Elf2
Name: E74-like factor 2
Synonyms: A230104O07Rik, 2610036A20Rik, NERF-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 69257
HGNC: HGNC:3317
Homologene: 5006
Samd4
Name: sterile alpha motif domain containing 4
Synonyms: Smaug, 1700024G08Rik, 1700111L17Rik, sunk, 4933436G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 74480
Homologene: 19167
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: D86 mRNA, PKHDL1, fibrocystin L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 192190
Homologene: 16332
Itgb4
Name: integrin beta 4
Synonyms: CD104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 192897
HGNC: HGNC:6158
Homologene: 179
Zan
Name: zonadhesin
Synonyms: Zan
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22635
Homologene: 124417
Rp1
Name: retinitis pigmentosa 1 (human)
Synonyms: Dcdc3, oxygen-regulated protein 1, Orp1, mG145, Rp1h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19888
Homologene: 4564
Vmn1r20
Name: vomeronasal 1 receptor 20
Synonyms: Gm5569
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 434017
Homologene: 79462
Olfr151
Name: olfactory receptor 151
Synonyms: GA_x6K02T2PVTD-31408136-31407207, MOR171-2, M71
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 406176
HGNC: HGNC:8469
Homologene: 12726
Mus81
Name: MUS81 structure-specific endonuclease subunit
Synonyms: 1200008A18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 71711
Homologene: 5725
Fbxw15
Name: F-box and WD-40 domain protein 15
Synonyms: Fbxo12J
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382105
Homologene: 110776
BC051665
Name: cDNA sequence BC051665
Synonyms: cathepsin L-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218275
VEGA: 13
Homologene: 74298
Ces1a
Name: carboxylesterase 1A
Synonyms: Gm4976
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244595
HGNC: HGNC:1863
Homologene: 134142
Zbtb39
Name: zinc finger and BTB domain containing 39
Synonyms: 7030401O21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 320080
VEGA: 10
Homologene: 8890
Vwa1
Name: von Willebrand factor A domain containing 1
Synonyms: 4932416A11Rik, WARP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 246228
Homologene: 11270
AC238840.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Gm12588
Name: predicted gene 12588
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 102632383
Homologene: 137828
Skiv2l-ps1
Name: superkiller viralicidic activity 2-like (S. cerevisiae), pseudogene 1
Synonyms: Ski2w2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 100418288
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 4,348,013 bp
  • A to G, chromosome 3 at 51,257,444 bp
  • A to G, chromosome 4 at 155,773,069 bp
  • G to T, chromosome 5 at 92,943,086 bp
  • A to G, chromosome 5 at 137,421,867 bp
  • A to G, chromosome 6 at 57,431,946 bp
  • T to A, chromosome 7 at 38,768,151 bp
  • CAAAAA to CAAAA, chromosome 8 at 84,716,247 bp
  • C to T, chromosome 8 at 93,025,319 bp
  • T to C, chromosome 9 at 21,467,556 bp
  • T to C, chromosome 9 at 37,711,365 bp
  • T to C, chromosome 9 at 109,565,432 bp
  • A to T, chromosome 10 at 127,741,581 bp
  • C to T, chromosome 11 at 103,930,752 bp
  • G to T, chromosome 11 at 116,000,429 bp
  • T to C, chromosome 11 at 121,906,168 bp
  • T to C, chromosome 12 at 31,972,150 bp
  • T to C, chromosome 13 at 60,783,960 bp
  • G to A, chromosome 14 at 46,884,842 bp
  • C to A, chromosome 15 at 44,560,506 bp
  • A to G, chromosome 17 at 34,770,102 bp
  • A to G, chromosome 17 at 53,610,904 bp
  • A to G, chromosome 19 at 5,484,963 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2346 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040329-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.