Strain Name:
C57BL/6J-MtgxR2348Btlr/Mmmh
Stock Number:
040330-MU
Citation ID:
RRID:MMRRC_040330-MU
Other Names:
R2348 (G1), C57BL/6J-MtgxR2348Btlr
Major Collection:

Gene Information

Arhgap10
Name: Rho GTPase activating protein 10
Synonyms: PSGAP-s, PSGAP-m, A930033B01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 78514
Homologene: 12695
Htr2a
Name: 5-hydroxytryptamine (serotonin) receptor 2A
Synonyms: 5-HT2A receptor, Htr2, Htr-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 15558
VEGA: 14
HGNC: HGNC:5293
Homologene: 68073
Rc3h1
Name: RING CCCH (C3H) domains 1
Synonyms: roquin, 5730557L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381305
Homologene: 19036
Itch
Name: itchy, E3 ubiquitin protein ligase
Synonyms: 8030492O04Rik, C230047C07Rik, 6720481N21Rik, AIP4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16396
Homologene: 88442
Khdc4
Name: KH domain containing 4, pre-mRNA splicing factor
Synonyms: 2810403A07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 74200
Homologene: 41761
Ift52
Name: intraflagellar transport 52
Synonyms: NGD5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 245866
Homologene: 9335
Adcyap1
Name: adenylate cyclase activating polypeptide 1
Synonyms: PACAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 11516
VEGA: 17
HGNC: HGNC:241
Homologene: 869
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Hdac5
Name: histone deacetylase 5
Synonyms: mHDA1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15184
Homologene: 3995
Shroom3
Name: shroom family member 3
Synonyms: Shrm, D5Ertd287e, Shrm3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 27428
Homologene: 9263
Dlgap4
Name: DLG associated protein 4
Synonyms: WBP16, Sapap4, DAP4, PSD-95/SAP90 binding protein 4, SAP90/PSD-95-associated protein 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228836
Homologene: 8935
Ctc1
Name: CTS telomere maintenance complex component 1
Synonyms: 1500010J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68964
Homologene: 11830
Dennd4c
Name: DENN/MADD domain containing 4C
Synonyms: 1700065A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329877
Homologene: 23057
C2cd3
Name: C2 calcium-dependent domain containing 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 277939
Homologene: 19524
Zfp457
Name: zinc finger protein 457
Synonyms: Rslcan-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 431706
Homologene: 75311
E4f1
Name: E4F transcription factor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13560
VEGA: 17
HGNC: HGNC:3121
Homologene: 3259
Naip5
Name: NLR family, apoptosis inhibitory protein 5
Synonyms: Naip-rs3, Birc1e, Lgn1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17951
HGNC: HGNC:7634
Homologene: 113589
Olfr1484
Name: olfactory receptor 1484
Synonyms: MOR202-37, GA_x6K02T2RE5P-3917859-3918806
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258288
Homologene: 110492
Usp40
Name: ubiquitin specific peptidase 40
Synonyms: B230215L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227334
Homologene: 32400
Klra13-ps
Name: killer cell lectin-like receptor subfamily A, member 13, pseudogene
Synonyms: Ly49M
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16631
Vmn2r88
Name: vomeronasal 2, receptor 88
Synonyms: V2r3, V2r13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 669149
Homologene: 129606
Tpk1
Name: thiamine pyrophosphokinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 29807
Homologene: 6960
Kiss1r
Name: KISS1 receptor
Synonyms: Gpr54
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 114229
VEGA: 10
HGNC: HGNC:4510
Homologene: 11411
Atp9a
Name: ATPase, class II, type 9A
Synonyms: Class II, IIa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11981
Homologene: 69194
Rasip1
Name: Ras interacting protein 1
Synonyms: Rain, 2610025P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 69903
Homologene: 9847
Krt8
Name: keratin 8
Synonyms: cytokeratin 8, Krt2-8, K8, Krt-2.8, EndoA, cytokeratin8, cytokeratin-8, Card2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16691
VEGA: 15
HGNC: HGNC:6446
Homologene: 55643
Ano3
Name: anoctamin 3
Synonyms: B230324K02Rik, Tmem16c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228432
Homologene: 57147
Ccdc130
Name: coiled-coil domain containing 130
Synonyms: 4930527D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67736
Homologene: 12183
Vps37c
Name: vacuolar protein sorting 37C
Synonyms: 5730409F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107305
VEGA: 19
Homologene: 45637
Asb18
Name: ankyrin repeat and SOCS box-containing 18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 208372
Homologene: 16382
Mycl
Name: v-myc avian myelocytomatosis viral oncogene lung carcinoma derived
Synonyms: L-myc, bHLHe38, Lmyc-1, Lmyc1, Mycl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 16918
HGNC: HGNC:7555
Homologene: 3921
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 87,974,067 bp
  • T to G, chromosome 1 at 90,014,534 bp
  • C to A, chromosome 1 at 160,950,860 bp
  • A to G, chromosome 2 at 110,783,743 bp
  • T to C, chromosome 2 at 155,209,078 bp
  • C to A, chromosome 2 at 156,701,206 bp
  • T to C, chromosome 2 at 163,045,257 bp
  • A to T, chromosome 2 at 168,710,826 bp
  • T to C, chromosome 3 at 88,708,876 bp
  • G to A, chromosome 4 at 86,811,527 bp
  • A to T, chromosome 4 at 122,996,952 bp
  • G to T, chromosome 5 at 92,943,086 bp
  • A to G, chromosome 6 at 43,346,844 bp
  • A to T, chromosome 6 at 130,291,308 bp
  • T to G, chromosome 7 at 45,629,083 bp
  • T to C, chromosome 7 at 100,413,366 bp
  • A to T, chromosome 8 at 77,450,926 bp
  • G to A, chromosome 8 at 84,259,262 bp
  • C to A, chromosome 9 at 53,492,268 bp
  • G to T, chromosome 10 at 79,921,820 bp
  • T to A, chromosome 11 at 69,026,191 bp
  • T to C, chromosome 11 at 102,200,014 bp
  • T to A, chromosome 13 at 67,293,404 bp
  • C to T, chromosome 13 at 100,219,738 bp
  • A to T, chromosome 14 at 51,414,004 bp
  • A to T, chromosome 14 at 74,645,110 bp
  • T to C, chromosome 15 at 101,998,865 bp
  • G to A, chromosome 17 at 24,444,767 bp
  • A to G, chromosome 17 at 93,202,274 bp
  • T to C, chromosome 19 at 10,706,300 bp
  • A to T, chromosome 19 at 13,586,189 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2348 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040330-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.