Strain Name:
C57BL/6J-MtgxR2350Btlr/Mmmh
Stock Number:
040332-MU
Citation ID:
RRID:MMRRC_040332-MU
Other Names:
R2350 (G1), C57BL/6J-MtgxR2350Btlr
Major Collection:

Gene Information

Adamts20
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223838
Homologene: 11808
Dennd2c
Name: DENN/MADD domain containing 2C
Synonyms: A930010I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 329727
Homologene: 19542
Nsf
Name: N-ethylmaleimide sensitive fusion protein
Synonyms: SKD2, N-ethylmaleimide sensitive factor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18195
HGNC: HGNC:8016
Homologene: 4502
Ago2
Name: argonaute RISC catalytic subunit 2
Synonyms: argonaute 2, 1110029L17Rik, 2310051F07Rik, Eif2c2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239528
HGNC: HGNC:3263
Homologene: 81825
Zfp292
Name: zinc finger protein 292
Synonyms: Zn-16, Zn-15, Krox-10, Zfp-15, 9430062L07Rik, 5730450D02Rik, Zfp15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 30046
Homologene: 8493
Ccdc138
Name: coiled-coil domain containing 138
Synonyms: 6230424H07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 76138
VEGA: 10
Homologene: 44912
Unc5b
Name: unc-5 netrin receptor B
Synonyms: 6330415E02Rik, Unc5h2, D10Bwg0792e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 107449
VEGA: 10
Homologene: 32538
Gucy2c
Name: guanylate cyclase 2c
Synonyms: GC-C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14917
HGNC: HGNC:4688
Homologene: 3641
Adam12
Name: a disintegrin and metallopeptidase domain 12 (meltrin alpha)
Synonyms: Mltna, ADAM12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11489
HGNC: HGNC:190
Homologene: 74862
Nmbr
Name: neuromedin B receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18101
HGNC: HGNC:7843
Homologene: 20560
Nfix
Name: nuclear factor I/X
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18032
HGNC: HGNC:7788
Homologene: 1872
Flcn
Name: folliculin
Synonyms: B430214A04Rik, BHD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216805
Homologene: 14583
Abca3
Name: ATP-binding cassette, sub-family A (ABC1), member 3
Synonyms: ABC-C, Abc3, 1810036E22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 27410
HGNC: HGNC:33
Homologene: 37437
Ppp2cb
Name: protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform
Synonyms: D8Ertd766e, PP2Ac
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 19053
HGNC: HGNC:9300
Homologene: 37889
Mocos
Name: molybdenum cofactor sulfurase
Synonyms: 1110018O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 68591
VEGA: 18
Homologene: 9931
Vmn2r6
Name: vomeronasal 2, receptor 6
Synonyms: EG620718, EG667069
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 667069
Homologene: 129754
Dnah3
Name: dynein, axonemal, heavy chain 3
Synonyms: Dnahc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381917
HGNC: HGNC:2949
Homologene: 19674
Scn9a
Name: sodium channel, voltage-gated, type IX, alpha
Synonyms: PN1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20274
Homologene: 2237
A2m
Name: alpha-2-macroglobulin
Synonyms: A2mp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232345
HGNC: HGNC:7
Homologene: 37248
Phkg1
Name: phosphorylase kinase gamma 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18682
HGNC: HGNC:8930
Homologene: 68508
Myom2
Name: myomesin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 17930
HGNC: HGNC:7614
Homologene: 2953
Fam234b
Name: family with sequence similarity 234, member B
Synonyms: 8430419L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74525
Homologene: 12572
Npepl1
Name: aminopeptidase-like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228961
Homologene: 11647
Alpk3
Name: alpha-kinase 3
Synonyms: Midori
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 116904
Homologene: 10813
Cyp2j6
Name: cytochrome P450, family 2, subfamily j, polypeptide 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13110
HGNC: HGNC:2634
Homologene: 68091
D630003M21Rik
Name: RIKEN cDNA D630003M21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228846
Homologene: 18598
Nfatc2ip
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 interacting protein
Synonyms: NIP45, D7Ertd304e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18020
Homologene: 7862
Parpbp
Name: PARP1 binding protein
Synonyms: 4930547N16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 75317
Homologene: 49519
Cntnap5b
Name: contactin associated protein-like 5B
Synonyms: Caspr5-2, C230078M14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241175
Homologene: 104295
Ccdc63
Name: coiled-coil domain containing 63
Synonyms: 4921511C16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330188
Homologene: 45136
Cpne7
Name: copine VII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102278
HGNC: HGNC:2320
Homologene: 65128
Vmn2r101
Name: vomeronasal 2, receptor 101
Synonyms: EG627576
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 627576
Homologene: 115024
Vmn1r71
Name: vomeronasal 1 receptor 71
Synonyms: V1re13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 252910
Homologene: 74352
Dqx1
Name: DEAQ RNA-dependent ATPase
Synonyms: 2310066E11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 93838
Homologene: 14143
Otop2
Name: otopetrin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237987
Homologene: 27803
Insl6
Name: insulin-like 6
Synonyms: RIF1, relaxin/insulin-like factor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 27356
HGNC: HGNC:6089
Homologene: 5197
Irx1
Name: Iroquois homeobox 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 16371
Homologene: 19065
Pcdhb20
Name: protocadherin beta 20
Synonyms: Pcdhb14, PcdhbT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93891
HGNC: HGNC:8685
Homologene: 134303
Gsdmc4
Name: gasdermin C4
Synonyms: 9030605I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 74548
HGNC: HGNC:7151
Homologene: 69487
Olfr867
Name: olfactory receptor 867
Synonyms: GA_x6K02T2PVTD-13795933-13794938, MOR143-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 257898
Homologene: 128152
Ubl7
Name: ubiquitin-like 7 (bone marrow stromal cell-derived)
Synonyms: 2300004C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 69459
VEGA: 9
Homologene: 12312
Cldn12
Name: claudin 12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 64945
HGNC: HGNC:2034
Homologene: 40809
Arpin
Name: actin-related protein 2/3 complex inhibitor
Synonyms: 2610034B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 70420
Homologene: 128480
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 100,379,126 bp
  • A to G, chromosome 2 at 66,504,968 bp
  • T to A, chromosome 2 at 158,201,011 bp
  • T to C, chromosome 2 at 174,111,773 bp
  • T to C, chromosome 3 at 64,556,352 bp
  • A to G, chromosome 3 at 103,132,001 bp
  • A to T, chromosome 4 at 34,811,281 bp
  • A to T, chromosome 4 at 96,529,408 bp
  • A to T, chromosome 5 at 5,507,845 bp
  • A to T, chromosome 5 at 122,122,885 bp
  • A to T, chromosome 5 at 129,864,532 bp
  • T to A, chromosome 6 at 83,059,087 bp
  • A to C, chromosome 6 at 121,678,088 bp
  • T to A, chromosome 6 at 135,231,724 bp
  • G to T, chromosome 6 at 136,763,074 bp
  • A to G, chromosome 7 at 10,747,919 bp
  • T to A, chromosome 7 at 79,931,805 bp
  • T to C, chromosome 7 at 81,094,970 bp
  • A to T, chromosome 7 at 120,045,788 bp
  • T to C, chromosome 7 at 126,395,998 bp
  • T to C, chromosome 7 at 133,919,524 bp
  • T to C, chromosome 8 at 15,108,835 bp
  • A to G, chromosome 8 at 33,611,827 bp
  • CAAAAA to CAAAA, chromosome 8 at 84,716,247 bp
  • A to G, chromosome 8 at 123,124,469 bp
  • G to T, chromosome 9 at 20,055,088 bp
  • A to T, chromosome 9 at 57,920,136 bp
  • AATA to AATAGATA, chromosome 10 at 14,760,718 bp
  • G to T, chromosome 10 at 58,561,893 bp
  • A to G, chromosome 10 at 60,778,200 bp
  • C to A, chromosome 10 at 88,133,088 bp
  • T to C, chromosome 11 at 59,792,659 bp
  • C to T, chromosome 11 at 103,930,752 bp
  • T to G, chromosome 11 at 115,326,850 bp
  • T to A, chromosome 13 at 71,960,048 bp
  • T to A, chromosome 15 at 63,893,165 bp
  • T to C, chromosome 15 at 73,119,461 bp
  • C to T, chromosome 15 at 94,283,916 bp
  • T to C, chromosome 17 at 19,589,783 bp
  • G to A, chromosome 17 at 24,400,394 bp
  • C to T, chromosome 18 at 24,666,656 bp
  • T to A, chromosome 18 at 37,504,510 bp
  • T to A, chromosome 19 at 29,325,245 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2350 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040332-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.