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Strain Name:
C57BL/6J-MtgxR2352Btlr/Mmmh
Stock Number:
040334-MU
Citation ID:
RRID:MMRRC_040334-MU
Other Names:
R2352 (G1), C57BL/6J-MtgxR2352Btlr
Major Collection:

Strain Information

Stx8
Name: syntaxin 8
Synonyms: 1110002H11Rik, 0610007H08Rik, 4930571E13Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 55943
Homologene: 37973
Dgat1
Name: diacylglycerol O-acyltransferase 1
Synonyms: D15Ertd23e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 13350
HGNC: HGNC:2843
Homologene: 7688
Gnl3
Name: guanine nucleotide binding protein nucleolar 3
Synonyms: nucleostemin, NS
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 30877
VEGA: 14
Homologene: 56670
Smc2
Name: structural maintenance of chromosomes 2
Synonyms: 5730502P04Rik, CAP-E, Fin16, Smc2l1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14211
Homologene: 4705
Sema4b
Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
Synonyms: SemC, Semac
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20352
Homologene: 8426
Scg3
Name: secretogranin III
Synonyms: SgIII, Chgd, 1B1075
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20255
VEGA: 9
Homologene: 7526
Tacr3
Name: tachykinin receptor 3
Synonyms: neuromedin K receptor, Tac3r, Nk3r
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 21338
Homologene: 824
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 139,457,562 bp
  • T to C, chromosome 2 at 52,287,336 bp
  • T to C, chromosome 2 at 156,335,355 bp
  • C to T, chromosome 3 at 59,240,692 bp
  • A to G, chromosome 3 at 88,104,508 bp
  • A to T, chromosome 3 at 94,429,160 bp
  • T to C, chromosome 3 at 134,854,870 bp
  • T to C, chromosome 3 at 145,507,762 bp
  • A to T, chromosome 4 at 52,460,266 bp
  • A to G, chromosome 4 at 55,008,313 bp
  • A to C, chromosome 4 at 58,951,869 bp
  • T to C, chromosome 5 at 34,669,176 bp
  • T to G, chromosome 5 at 107,433,441 bp
  • G to T, chromosome 5 at 130,506,152 bp
  • A to G, chromosome 5 at 144,173,911 bp
  • A to T, chromosome 6 at 48,568,872 bp
  • A to T, chromosome 6 at 129,269,604 bp
  • T to A, chromosome 6 at 136,722,556 bp
  • T to A, chromosome 7 at 4,149,410 bp
  • T to C, chromosome 7 at 25,306,173 bp
  • A to G, chromosome 7 at 29,535,898 bp
  • A to G, chromosome 7 at 64,297,580 bp
  • C to A, chromosome 7 at 80,220,879 bp
  • T to C, chromosome 7 at 143,497,415 bp
  • G to T, chromosome 8 at 3,192,593 bp
  • G to A, chromosome 8 at 10,594,905 bp
  • A to C, chromosome 8 at 46,209,175 bp
  • T to C, chromosome 9 at 20,470,105 bp
  • T to A, chromosome 9 at 54,393,862 bp
  • A to G, chromosome 9 at 75,661,396 bp
  • A to T, chromosome 10 at 41,482,233 bp
  • CGGG to CGGGGGG, chromosome 11 at 5,201,791 bp
  • G to A, chromosome 11 at 58,612,108 bp
  • A to T, chromosome 11 at 58,612,850 bp
  • G to A, chromosome 11 at 67,220,537 bp
  • T to A, chromosome 11 at 67,284,742 bp
  • A to G, chromosome 11 at 67,973,251 bp
  • T to A, chromosome 11 at 72,252,321 bp
  • G to A, chromosome 11 at 85,482,748 bp
  • A to G, chromosome 11 at 96,366,377 bp
  • C to G, chromosome 11 at 100,086,659 bp
  • T to C, chromosome 11 at 115,329,101 bp
  • G to A, chromosome 12 at 79,284,116 bp
  • A to T, chromosome 12 at 117,928,330 bp
  • A to T, chromosome 13 at 15,662,392 bp
  • A to C, chromosome 13 at 23,232,513 bp
  • A to G, chromosome 13 at 27,575,589 bp
  • A to T, chromosome 13 at 61,352,772 bp
  • A to G, chromosome 13 at 81,508,642 bp
  • C to T, chromosome 14 at 31,016,826 bp
  • C to T, chromosome 14 at 31,710,947 bp
  • C to T, chromosome 14 at 54,440,545 bp
  • A to G, chromosome 15 at 43,867,110 bp
  • T to C, chromosome 15 at 76,502,313 bp
  • T to C, chromosome 15 at 99,794,515 bp
  • A to T, chromosome 16 at 36,898,559 bp
  • C to T, chromosome 17 at 26,742,346 bp
  • T to A, chromosome 17 at 31,193,446 bp
  • A to T, chromosome 17 at 32,059,328 bp
  • C to T, chromosome 17 at 88,742,299 bp
  • T to C, chromosome 18 at 77,406,008 bp
  • A to G, chromosome 19 at 16,873,069 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2352 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040334-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.