Strain Name:
C57BL/6J-MtgxR2353Btlr/Mmmh
Stock Number:
040335-MU
Citation ID:
RRID:MMRRC_040335-MU
Other Names:
R2353 (G1), C57BL/6J-MtgxR2353Btlr
Major Collection:

Gene Information

Sin3b
Name: transcriptional regulator, SIN3B (yeast)
Synonyms: 2810430C10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20467
Homologene: 81810
Aspm
Name: abnormal spindle microtubule assembly
Synonyms: Sha1, Aspm, D330028K02Rik, MCPH5, Calmbp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12316
Homologene: 7650
Zfp407
Name: zinc finger protein 407
Synonyms: LOC240469, LOC381139, 6430585N13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240476
Homologene: 86402
Hip1
Name: huntingtin interacting protein 1
Synonyms: 2610109B09Rik, A930014B11Rik, E130315I21Rik, HIP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 215114
HGNC: HGNC:4913
Homologene: 68463
Scn10a
Name: sodium channel, voltage-gated, type X, alpha
Synonyms: Nav1.8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20264
VEGA: 9
Homologene: 21300
Ubr4
Name: ubiquitin protein ligase E3 component n-recognin 4
Synonyms: A930005E13Rik, LOC381562, D930005K06Rik, 1810009A16Rik, Zubr1, p600
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 69116
Homologene: 10804
Lrrc4
Name: leucine rich repeat containing 4
Synonyms: Nag14, NGL-2, NGL2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 192198
Homologene: 36403
Garnl3
Name: GTPase activating RANGAP domain-like 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99326
Homologene: 13003
Ppwd1
Name: peptidylprolyl isomerase domain and WD repeat containing 1
Synonyms: A330090G21Rik, 4632422M10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238831
VEGA: 13
Homologene: 9099
Znrf3
Name: zinc and ring finger 3
Synonyms: LOC382477
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 407821
Homologene: 46592
Uts2
Name: urotensin 2
Synonyms: urotensin II precursor, prepro-UII, Ucn2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 24111
Homologene: 4939
Enpp1
Name: ectonucleotide pyrophosphatase/phosphodiesterase 1
Synonyms: PC-1, NPP1, Ly-41, Npps, Pca-1, Pca, Pdnp1, twy, CD203c, E-NPP1, 4833416E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18605
HGNC: HGNC:3356
Homologene: 38151
Apeh
Name: acylpeptide hydrolase
Synonyms: N-acylaminoacyl peptide hydrolase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235606
HGNC: HGNC:586
Homologene: 1240
B4galt3
Name: UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3
Synonyms: beta4GalT-III, ESTM26, 9530061M23Rik, R74981
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 57370
HGNC: HGNC:926
Homologene: 48241
Aadacl4
Name: arylacetamide deacetylase like 4
Synonyms: Gm13177
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 435815
Homologene: 82530
Ac127374.1
Name: Uncharacterized protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Gbe1
Name: glucan (1,4-alpha-), branching enzyme 1
Synonyms: 2310045H19Rik, 2810426P10Rik, D16Ertd536e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74185
HGNC: HGNC:4180
Homologene: 129
Ap3b2
Name: adaptor-related protein complex 3, beta 2 subunit
Synonyms: Naptb, beta3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11775
HGNC: HGNC:567
Homologene: 55837
Olfr394
Name: olfactory receptor 394
Synonyms: GA_x6K02T2P1NL-4043306-4042374, MOR135-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259009
Homologene: 133669
Zfp712
Name: zinc finger protein 712
Synonyms: 4921504N20Rik, mszf31, mszf89
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 78251
VEGA: 13
Homologene: 136296
Adam7
Name: a disintegrin and metallopeptidase domain 7
Synonyms: EAP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 11500
HGNC: HGNC:214
Homologene: 2830
Parl
Name: presenilin associated, rhomboid-like
Synonyms: PSENIP2, PRO2207, PSARL1, D16Ertd607e, Psarl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 381038
Homologene: 10239
Cdh15
Name: cadherin 15
Synonyms: Cdh14, Mcad, M cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12555
HGNC: HGNC:1754
Homologene: 3622
Zfp109
Name: zinc finger protein 109
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56869
Ankk1
Name: ankyrin repeat and kinase domain containing 1
Synonyms: 9930020N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244859
VEGA: 9
Homologene: 18258
Sh3rf3
Name: SH3 domain containing ring finger 3
Synonyms: 4831416G18Rik, Sh3md4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237353
Homologene: 77551
Cd163
Name: CD163 antigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 93671
HGNC: HGNC:1631
Homologene: 128811
Cd38
Name: CD38 antigen
Synonyms: Cd38-rs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12494
HGNC: HGNC:1667
Homologene: 1345
Hspa14
Name: heat shock protein 14
Synonyms: NST-1, 70kDa, HSP70L1, Hsp70-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 50497
Homologene: 74307
Med31
Name: mediator complex subunit 31
Synonyms: 3110004H13Rik, l11Jus15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67279
Homologene: 9368
AF366264
Name: cDNA sequence AF366264
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 231201
Homologene: 130042
Fech
Name: ferrochelatase
Synonyms: fch, Fcl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 14151
VEGA: 18
HGNC: HGNC:3647
Homologene: 113
Olfr1270
Name: olfactory receptor 1270
Synonyms: GA_x6K02T2Q125-51584440-51583526, MOR227-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258987
HGNC: HGNC:8290
Homologene: 133648
Mmel1
Name: membrane metallo-endopeptidase-like 1
Synonyms: Nl1, NEPLP gamma, NEPLP beta, NEPLP alpha, SEP, Mell1, Nep2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 27390
Homologene: 22778
4930430O22Rik
Name: RIKEN cDNA 4930430O22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Vmn2r-ps117
Name: vomeronasal 2, receptor, pseudogene 117
Synonyms: EG665303
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 665303
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 139,477,697 bp
  • C to A, chromosome 1 at 171,274,043 bp
  • G to A, chromosome 2 at 3,511,176 bp
  • T to A, chromosome 2 at 33,064,034 bp
  • A to T, chromosome 2 at 90,149,718 bp
  • T to C, chromosome 4 at 139,433,673 bp
  • G to T, chromosome 4 at 144,623,209 bp
  • T to A, chromosome 4 at 151,000,136 bp
  • G to T, chromosome 4 at 154,882,255 bp
  • T to A, chromosome 5 at 43,908,011 bp
  • C to A, chromosome 5 at 115,436,509 bp
  • A to T, chromosome 5 at 135,412,712 bp
  • A to G, chromosome 6 at 28,831,452 bp
  • G to T, chromosome 6 at 124,319,156 bp
  • T to C, chromosome 7 at 24,229,381 bp
  • C to T, chromosome 7 at 81,473,850 bp
  • T to A, chromosome 8 at 13,836,951 bp
  • A to G, chromosome 8 at 31,739,195 bp
  • T to C, chromosome 8 at 72,724,152 bp
  • G to A, chromosome 8 at 122,862,024 bp
  • A to C, chromosome 9 at 49,418,690 bp
  • T to C, chromosome 9 at 108,086,292 bp
  • T to A, chromosome 9 at 119,638,687 bp
  • C to A, chromosome 10 at 24,651,341 bp
  • A to G, chromosome 10 at 59,007,073 bp
  • T to C, chromosome 11 at 5,281,170 bp
  • T to A, chromosome 11 at 72,214,140 bp
  • T to C, chromosome 11 at 73,887,834 bp
  • A to T, chromosome 13 at 67,061,049 bp
  • T to C, chromosome 13 at 104,213,582 bp
  • T to A, chromosome 14 at 68,505,088 bp
  • T to C, chromosome 16 at 20,287,040 bp
  • A to G, chromosome 16 at 70,437,021 bp
  • A to G, chromosome 17 at 18,827,107 bp
  • A to G, chromosome 18 at 64,458,225 bp
  • A to G, chromosome 18 at 84,559,880 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2353 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040335-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.