Strain Name:
C57BL/6J-MtgxR2369Btlr/Mmmh
Stock Number:
040349-MU
Citation ID:
RRID:MMRRC_040349-MU
Other Names:
R2369 (G1), C57BL/6J-MtgxR2369Btlr
Major Collection:

Gene Information

Met
Name: met proto-oncogene
Synonyms: c-Met, HGF receptor, Par4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Npl
Name: N-acetylneuraminate pyruvate lyase
Synonyms: 0610033B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74091
Homologene: 12533
Esco2
Name: establishment of sister chromatid cohesion N-acetyltransferase 2
Synonyms: 2410004I17Rik, D030072L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71988
Homologene: 12432
Xpo7
Name: exportin 7
Synonyms: Ranbp16, 4930506C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 65246
VEGA: 14
Homologene: 22857
Ing3
Name: inhibitor of growth family, member 3
Synonyms: 1300013A07Rik, P47ING3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 71777
Homologene: 6804
Rad1
Name: RAD1 checkpoint DNA exonuclease
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 19355
HGNC: HGNC:9806
Homologene: 37695
Pros1
Name: protein S (alpha)
Synonyms: protein S
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 19128
HGNC: HGNC:9456
Homologene: 264
Hbs1l
Name: Hbs1-like (S. cerevisiae)
Synonyms: eRFS, 2810035F15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 56422
VEGA: 10
HGNC: HGNC:4834
Homologene: 68525
Ahcyl1
Name: S-adenosylhomocysteine hydrolase-like 1
Synonyms: IRBIT, 1110034F20Rik, DCAL, Ahcy-rs3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229709
HGNC: HGNC:344
Homologene: 77353
Vit
Name: vitrin
Synonyms: akhirin, 2810429K11Rik, 1700110E08Rik, 1700052E02Rik, AKH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 74199
VEGA: 17
Homologene: 24942
Dclk3
Name: doublecortin-like kinase 3
Synonyms: Dcamkl3, Click-I, -II related
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 245038
Homologene: 70580
Wdr72
Name: WD repeat domain 72
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 546144
Homologene: 52326
Cubn
Name: cubilin (intrinsic factor-cobalamin receptor)
Synonyms: D2Wsu88e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 65969
HGNC: HGNC:2548
Homologene: 37434
Cdc42bpa
Name: CDC42 binding protein kinase alpha
Synonyms: DMPK-like, A930014J19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226751
HGNC: HGNC:1737
Homologene: 55765
Gbp5
Name: guanylate binding protein 5
Synonyms: Gbp5a, 5330409J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229898
Homologene: 14183
A2ml1
Name: alpha-2-macroglobulin like 1
Synonyms: BC048546, Ovos2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232400
Homologene: 45969
Grik5
Name: glutamate receptor, ionotropic, kainate 5 (gamma 2)
Synonyms: GluRgamma2, KA2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14809
HGNC: HGNC:4583
Homologene: 1578
Celsr3
Name: cadherin, EGF LAG seven-pass G-type receptor 3
Synonyms: Fmi1, Adgrc3, flamingo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 107934
HGNC: HGNC:3230
Homologene: 1077
Notch4
Name: notch 4
Synonyms: Int3, N4, Int-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18132
HGNC: HGNC:7884
Homologene: 3351
Zfp11
Name: zinc finger protein 11
Synonyms: Krox-6, Zfp-11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22648
Homologene: 87241
Cryba1
Name: crystallin, beta A1
Synonyms: Cryb, BA3/A1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12957
HGNC: HGNC:2394
Homologene: 3815
Atxn7l1
Name: ataxin 7-like 1
Synonyms: Atxn7l4, 2810423G08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 380753
Homologene: 53366
Ccdc33
Name: coiled-coil domain containing 33
Synonyms: LOC382077, 4930535E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382077
Homologene: 19641
Dip2a
Name: disco interacting protein 2 homolog A
Synonyms: Kiaa0184-hp, 4931420H10Rik, Dip2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 64451
Homologene: 41012
Tmem232
Name: transmembrane protein 232
Synonyms: E130009J12Rik, LOC381107
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 381107
VEGA: 17
Homologene: 54378
Lmtk3
Name: lemur tyrosine kinase 3
Synonyms: Aatyk3, AATYK3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381983
Homologene: 79449
9230112D13Rik
Name: RIKEN cDNA 9230112D13 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 78243
VEGA: 14
Ndufaf7
Name: NADH:ubiquinone oxidoreductase complex assembly factor 7
Synonyms: 2410091C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 73694
VEGA: 17
Homologene: 12508
Rassf4
Name: Ras association (RalGDS/AF-6) domain family member 4
Synonyms: 3830411C14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 213391
Homologene: 12926
Paqr6
Name: progestin and adipoQ receptor family member VI
Synonyms: 1500001B10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 68957
Homologene: 19746
Dok6
Name: docking protein 6
Synonyms: Dok-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 623279
Homologene: 45143
1700066M21Rik
Name: RIKEN cDNA 1700066M21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 73467
Homologene: 13624
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 57,382,791 bp
  • A to G, chromosome 1 at 153,518,877 bp
  • T to A, chromosome 1 at 180,111,732 bp
  • T to A, chromosome 2 at 13,491,217 bp
  • T to C, chromosome 3 at 88,365,953 bp
  • T to C, chromosome 3 at 107,670,240 bp
  • A to T, chromosome 3 at 142,500,719 bp
  • G to A, chromosome 5 at 129,656,465 bp
  • G to A, chromosome 6 at 17,531,528 bp
  • A to G, chromosome 6 at 21,950,091 bp
  • A to G, chromosome 6 at 116,638,297 bp
  • C to T, chromosome 6 at 128,580,386 bp
  • T to C, chromosome 7 at 25,058,537 bp
  • A to T, chromosome 7 at 45,795,088 bp
  • A to T, chromosome 9 at 58,076,630 bp
  • A to G, chromosome 9 at 74,210,175 bp
  • G to A, chromosome 9 at 108,842,552 bp
  • A to G, chromosome 9 at 111,488,542 bp
  • T to A, chromosome 10 at 21,307,745 bp
  • T to C, chromosome 10 at 76,313,196 bp
  • A to T, chromosome 11 at 77,722,601 bp
  • T to C, chromosome 12 at 33,358,850 bp
  • A to T, chromosome 14 at 34,511,956 bp
  • G to A, chromosome 14 at 65,821,740 bp
  • G to A, chromosome 14 at 70,687,731 bp
  • C to A, chromosome 15 at 10,486,659 bp
  • A to G, chromosome 16 at 62,928,069 bp
  • A to G, chromosome 17 at 34,585,950 bp
  • A to T, chromosome 17 at 65,402,997 bp
  • G to A, chromosome 17 at 78,566,005 bp
  • C to A, chromosome 17 at 78,945,032 bp
  • G to C, chromosome 18 at 89,414,864 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2369 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040349-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.