Strain Name:
C57BL/6J-MtgxR2395Btlr/Mmmh
Stock Number:
040363-MU
Citation ID:
RRID:MMRRC_040363-MU
Other Names:
R2395 (G1), C57BL/6J-MtgxR2395Btlr
Major Collection:

Gene Information

Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231051
Homologene: 46480
Actn4
Name: actinin alpha 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 60595
HGNC: HGNC:166
Homologene: 55857
Micu1
Name: mitochondrial calcium uptake 1
Synonyms: C730016L05Rik, Cbara1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216001
HGNC: HGNC:1530
Homologene: 4431
Srebf2
Name: sterol regulatory element binding factor 2
Synonyms: SREBP-2, nuc, SREBP2, SREBP2gc, bHLHd2, lop13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20788
VEGA: 15
Homologene: 20966
Dcp1b
Name: decapping mRNA 1B
Synonyms: B930050E02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 319618
Homologene: 51881
Rxrb
Name: retinoid X receptor beta
Synonyms: RCoR-1, H-2RIIBP, Nr2b2, Rub
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20182
Homologene: 7923
Cntn2
Name: contactin 2
Synonyms: Tax, TAG-1, axonin, D130012K04Rik, TAG1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21367
HGNC: HGNC:2172
Homologene: 3720
Nup54
Name: nucleoporin 54
Synonyms: 3110079L04Rik, 54kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269113
Homologene: 41169
Acsl3
Name: acyl-CoA synthetase long-chain family member 3
Synonyms: 2610510B12Rik, C85929, Facl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74205
HGNC: HGNC:3570
Homologene: 3278
Trpm2
Name: transient receptor potential cation channel, subfamily M, member 2
Synonyms: Trrp7, LTRPC2, TRPC7, 9830168K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 28240
Homologene: 20709
Mlph
Name: melanophilin
Synonyms: Slac-2a, D1Wsu84e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 171531
Homologene: 11465
Dnah6
Name: dynein, axonemal, heavy chain 6
Synonyms: A730004I20Rik, Dnahc6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330355
HGNC: HGNC:2951
Homologene: 15221
Myh15
Name: myosin, heavy chain 15
Synonyms: EG667772
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 667772
VEGA: 16
Homologene: 18929
Myh13
Name: myosin, heavy polypeptide 13, skeletal muscle
Synonyms: extraocular myosin, EO Myosin, MyHC-eo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 544791
HGNC: HGNC:7571
Homologene: 55780
Ush2a
Name: usherin
Synonyms: MUSH2A, A930011D15Rik, LOC269160, LOC381317, A930037M10Rik, Ushrn, Ush2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22283
Homologene: 66151
Olfr118
Name: olfactory receptor 118
Synonyms: GA_x6K02T2PSCP-2131124-2132089, MOR263-13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 404308
Homologene: 110622
Map3k10
Name: mitogen-activated protein kinase kinase kinase 10
Synonyms: Mlk2, serine/threonine kinase, MKN28 derived nonreceptor_type, mixed lineage kinase 2, MKN28 kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269881
HGNC: HGNC:6849
Homologene: 1834
Fam214b
Name: family with sequence similarity 214, member B
Synonyms: B230312A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230088
Homologene: 32625
Naip1
Name: NLR family, apoptosis inhibitory protein 1
Synonyms: D13Lsd1, Naip, Birc1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17940
VEGA: 13
HGNC: HGNC:7634
Homologene: 113589
Abcc9
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 9
Synonyms: SUR2B, SUR2A, Sur2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20928
HGNC: HGNC:60
Homologene: 56521
Phf8-ps
Name: PHD finger protein 8, pseudogene
Synonyms: 4921501E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 74042
VEGA: 17
Homologene: 66275
Abca8a
Name: ATP-binding cassette, sub-family A (ABC1), member 8a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217258
HGNC: HGNC:38
Homologene: 131160
Hltf
Name: helicase-like transcription factor
Synonyms: P113, Snf2l3, Smarca3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20585
Homologene: 30136
Vmn2r73
Name: vomeronasal 2, receptor 73
Synonyms: EG620928
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 620928
Homologene: 115466
B3glct
Name: beta-3-glucosyltransferase
Synonyms: LOC381694, B3galtl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 381694
Homologene: 14978
Abcc3
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 3
Synonyms: 1700019L09Rik, MRP3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76408
HGNC: HGNC:54
Homologene: 68364
Tmprss5
Name: transmembrane protease, serine 5 (spinesin)
Synonyms: spinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 80893
Homologene: 69471
Prss40
Name: protease, serine 40
Synonyms: Tesp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21756
Homologene: 69043
Fmn1
Name: formin 1
Synonyms: Fmn, formin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14260
HGNC: HGNC:3768
Homologene: 121778
P2rx2
Name: purinergic receptor P2X, ligand-gated ion channel, 2
Synonyms: P2X2a, P2x2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231602
Homologene: 14251
Nab1
Name: Ngfi-A binding protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17936
HGNC: HGNC:7626
Homologene: 4352
Olfr1056
Name: olfactory receptor 1056
Synonyms: GA_x6K02T2Q125-47827833-47826892, MOR186-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 259020
Homologene: 27328
Cblc
Name: Casitas B-lineage lymphoma c
Synonyms: Cbl3, 2310079L19Rik, 2310076I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 80794
Homologene: 8108
Riox1
Name: ribosomal oxygenase 1
Synonyms: NO66, 2410016O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 71952
VEGA: 12
Homologene: 11366
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 34,559,905 bp
  • A to G, chromosome 1 at 52,490,582 bp
  • T to C, chromosome 1 at 78,705,368 bp
  • A to G, chromosome 1 at 90,933,506 bp
  • T to C, chromosome 1 at 132,526,372 bp
  • C to T, chromosome 1 at 188,947,040 bp
  • A to G, chromosome 2 at 86,356,265 bp
  • A to G, chromosome 2 at 113,365,181 bp
  • C to T, chromosome 3 at 20,092,742 bp
  • C to A, chromosome 4 at 43,035,964 bp
  • T to C, chromosome 5 at 25,315,152 bp
  • A to G, chromosome 5 at 92,431,027 bp
  • A to G, chromosome 5 at 110,341,661 bp
  • A to G, chromosome 5 at 149,754,186 bp
  • T to C, chromosome 6 at 73,091,967 bp
  • T to C, chromosome 6 at 119,215,064 bp
  • C to A, chromosome 6 at 142,620,669 bp
  • C to A, chromosome 7 at 19,785,380 bp
  • T to C, chromosome 7 at 27,673,993 bp
  • G to A, chromosome 7 at 28,898,266 bp
  • A to T, chromosome 7 at 85,857,767 bp
  • T to A, chromosome 9 at 49,115,135 bp
  • G to T, chromosome 10 at 59,863,202 bp
  • A to C, chromosome 10 at 77,947,880 bp
  • A to G, chromosome 11 at 67,364,922 bp
  • A to G, chromosome 11 at 94,357,306 bp
  • A to G, chromosome 11 at 110,068,788 bp
  • A to T, chromosome 12 at 83,950,644 bp
  • T to C, chromosome 13 at 100,423,106 bp
  • C to T, chromosome 15 at 82,192,255 bp
  • A to G, chromosome 16 at 49,069,514 bp
  • T to C, chromosome 17 at 33,065,962 bp
  • T to G, chromosome 17 at 34,037,438 bp
  • T to G, chromosome 17 at 37,672,696 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2395 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040363-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.