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Strain Name:
C57BL/6J-MtgxR2418Btlr/Mmmh
Stock Number:
040380-MU
Citation ID:
RRID:MMRRC_040380-MU
Other Names:
R2418 (G1), C57BL/6J-MtgxR2418Btlr
Major Collection:

Strain Information

Itk
Name: IL2 inducible T cell kinase
Synonyms: Emt, Tsk, Tcsk
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16428
HGNC: HGNC:6171
Homologene: 4051
Resp18
Name: regulated endocrine-specific protein 18
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19711
Homologene: 7516
Magi1
Name: membrane associated guanylate kinase, WW and PDZ domain containing 1
Synonyms: WWP3, Gukmi1, AIP3, BAP1, Baiap1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14924
HGNC: HGNC:946
Homologene: 31257
Smyd1
Name: SET and MYND domain containing 1
Synonyms: Bop, 4632404M21Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12180
Homologene: 7645
Lactb2
Name: lactamase, beta 2
Synonyms: E430032H21Rik, Cgi-83
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 212442
Homologene: 9349
Rps6ka2
Name: ribosomal protein S6 kinase, polypeptide 2
Synonyms: Rsk3, Rps6ka-rs1, 90kDa, p90rsk, pp90rsk, D17Wsu134e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20112
VEGA: 17
Homologene: 100680
Cnot8
Name: CCR4-NOT transcription complex, subunit 8
Synonyms: 1810022F04Rik, 1500015I04Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69125
HGNC: HGNC:9207
Homologene: 48304
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 13,660,339 bp
  • T to C, chromosome 1 at 75,272,311 bp
  • G to T, chromosome 1 at 82,532,936 bp
  • A to T, chromosome 1 at 162,966,958 bp
  • G to A, chromosome 2 at 25,437,989 bp
  • T to C, chromosome 2 at 66,273,843 bp
  • A to G, chromosome 2 at 88,959,036 bp
  • C to T, chromosome 2 at 132,824,738 bp
  • G to T, chromosome 3 at 93,445,629 bp
  • G to A, chromosome 4 at 11,961,838 bp
  • A to G, chromosome 4 at 109,066,218 bp
  • A to G, chromosome 5 at 35,604,638 bp
  • T to C, chromosome 5 at 110,201,868 bp
  • A to C, chromosome 5 at 123,254,268 bp
  • A to G, chromosome 6 at 42,838,049 bp
  • A to T, chromosome 6 at 71,239,553 bp
  • T to C, chromosome 6 at 93,745,910 bp
  • T to C, chromosome 6 at 131,687,447 bp
  • A to T, chromosome 7 at 23,541,399 bp
  • A to G, chromosome 7 at 24,745,267 bp
  • A to G, chromosome 7 at 26,186,707 bp
  • C to A, chromosome 7 at 79,698,693 bp
  • A to T, chromosome 7 at 104,579,934 bp
  • T to C, chromosome 7 at 106,603,447 bp
  • G to T, chromosome 7 at 112,320,734 bp
  • C to T, chromosome 7 at 128,142,333 bp
  • T to A, chromosome 8 at 10,396,839 bp
  • C to T, chromosome 8 at 21,998,374 bp
  • A to T, chromosome 8 at 45,289,112 bp
  • C to T, chromosome 8 at 48,276,658 bp
  • T to C, chromosome 8 at 94,777,408 bp
  • A to C, chromosome 8 at 109,670,721 bp
  • C to A, chromosome 9 at 22,129,952 bp
  • T to C, chromosome 9 at 46,276,192 bp
  • T to A, chromosome 10 at 20,110,857 bp
  • T to C, chromosome 10 at 79,859,677 bp
  • T to C, chromosome 10 at 93,284,827 bp
  • A to G, chromosome 11 at 20,064,866 bp
  • A to G, chromosome 11 at 46,338,217 bp
  • G to A, chromosome 11 at 58,115,310 bp
  • C to A, chromosome 11 at 60,748,016 bp
  • A to T, chromosome 11 at 66,095,415 bp
  • G to A, chromosome 11 at 83,035,611 bp
  • A to C, chromosome 11 at 97,059,178 bp
  • T to C, chromosome 12 at 72,304,278 bp
  • T to A, chromosome 12 at 72,556,263 bp
  • A to G, chromosome 12 at 103,417,737 bp
  • T to A, chromosome 13 at 22,524,834 bp
  • T to G, chromosome 13 at 22,556,250 bp
  • C to T, chromosome 13 at 43,549,537 bp
  • T to A, chromosome 14 at 7,968,089 bp
  • A to G, chromosome 14 at 20,311,513 bp
  • G to A, chromosome 14 at 31,641,136 bp
  • G to T, chromosome 15 at 4,574,763 bp
  • A to G, chromosome 15 at 79,365,943 bp
  • A to G, chromosome 15 at 89,161,069 bp
  • C to A, chromosome 15 at 91,797,526 bp
  • A to C, chromosome 15 at 98,907,537 bp
  • T to C, chromosome 15 at 101,946,634 bp
  • A to C, chromosome 17 at 7,299,339 bp
  • A to T, chromosome 17 at 34,977,188 bp
  • T to C, chromosome 18 at 37,989,944 bp
  • C to T, chromosome 19 at 3,807,266 bp
  • T to C, chromosome 19 at 44,131,472 bp
  • A to G, chromosome X at 48,370,541 bp
  • A to T, chromosome X at 154,575,227 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2418 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040380-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.