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Strain Name:
C57BL/6J-MtgxR2421Btlr/Mmmh
Stock Number:
040383-MU
Citation ID:
RRID:MMRRC_040383-MU
Other Names:
R2421 (G1), C57BL/6J-MtgxR2421Btlr
Major Collection:

Strain Information

Vangl2
Name: VANGL planar cell polarity 2
Synonyms: Lpp1, C530001F03Rik, skam17Jus, Ltap, strabismus, loop-tail, ska17, Lootl
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 93840
Homologene: 62161
Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, sAC, 4930431D04Rik, Sacy
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 271639
Homologene: 10188
Arhgap39
Name: Rho GTPase activating protein 39
Synonyms: D15Wsu169e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223666
Homologene: 27825
Pef1
Name: penta-EF hand domain containing 1
Synonyms: 2600002E23Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67898
Homologene: 56569
Arhgef12
Name: Rho guanine nucleotide exchange factor 12
Synonyms: LARG, 2310014B11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69632
VEGA: 9
Homologene: 9088
Dnajc21
Name: DnaJ heat shock protein family (Hsp40) member C21
Synonyms: 9930116P15Rik, 4930461P20Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78244
Homologene: 6752
Spred1
Name: sprouty protein with EVH-1 domain 1, related sequence
Synonyms: Spred-1, 5730461F13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 114715
Homologene: 24919
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 37,613,475 bp
  • G to A, chromosome 1 at 55,052,977 bp
  • G to A, chromosome 1 at 82,670,275 bp
  • T to C, chromosome 1 at 134,327,400 bp
  • G to A, chromosome 1 at 139,488,487 bp
  • T to A, chromosome 1 at 165,558,597 bp
  • T to C, chromosome 1 at 172,007,959 bp
  • C to A, chromosome 1 at 179,533,666 bp
  • G to A, chromosome 2 at 31,185,529 bp
  • T to C, chromosome 2 at 40,882,133 bp
  • A to T, chromosome 2 at 66,726,302 bp
  • T to C, chromosome 2 at 89,074,992 bp
  • T to C, chromosome 2 at 117,165,010 bp
  • T to C, chromosome 2 at 119,655,737 bp
  • T to A, chromosome 2 at 162,278,040 bp
  • A to G, chromosome 3 at 126,780,415 bp
  • C to T, chromosome 4 at 3,748,787 bp
  • T to C, chromosome 4 at 12,095,127 bp
  • T to A, chromosome 4 at 32,723,621 bp
  • C to A, chromosome 4 at 130,127,317 bp
  • T to C, chromosome 5 at 5,455,912 bp
  • G to A, chromosome 5 at 35,512,399 bp
  • A to G, chromosome 5 at 86,132,616 bp
  • A to G, chromosome 5 at 89,683,175 bp
  • C to T, chromosome 5 at 100,812,174 bp
  • A to G, chromosome 5 at 106,637,244 bp
  • A to C, chromosome 5 at 108,228,588 bp
  • A to G, chromosome 5 at 109,086,532 bp
  • A to T, chromosome 5 at 121,802,079 bp
  • A to T, chromosome 5 at 130,269,260 bp
  • T to A, chromosome 6 at 116,493,713 bp
  • A to G, chromosome 6 at 119,215,266 bp
  • G to A, chromosome 7 at 107,436,781 bp
  • A to T, chromosome 8 at 68,461,508 bp
  • T to A, chromosome 8 at 79,281,432 bp
  • T to A, chromosome 8 at 109,709,533 bp
  • A to G, chromosome 9 at 39,950,528 bp
  • A to T, chromosome 9 at 43,001,006 bp
  • T to C, chromosome 9 at 73,931,547 bp
  • T to A, chromosome 9 at 119,902,404 bp
  • G to A, chromosome 10 at 3,958,048 bp
  • T to C, chromosome 10 at 23,992,517 bp
  • T to C, chromosome 10 at 69,982,204 bp
  • A to C, chromosome 10 at 78,173,469 bp
  • T to C, chromosome 10 at 81,091,818 bp
  • T to A, chromosome 10 at 83,579,521 bp
  • A to T, chromosome 10 at 91,020,723 bp
  • T to A, chromosome 11 at 72,755,896 bp
  • A to G, chromosome 11 at 84,009,224 bp
  • A to T, chromosome 11 at 100,120,051 bp
  • T to A, chromosome 11 at 102,287,755 bp
  • G to A, chromosome 11 at 120,462,264 bp
  • A to G, chromosome 12 at 51,999,855 bp
  • C to A, chromosome 12 at 83,116,283 bp
  • A to G, chromosome 12 at 101,042,653 bp
  • T to A, chromosome 12 at 113,369,487 bp
  • T to G, chromosome 13 at 11,591,237 bp
  • T to C, chromosome 13 at 49,900,663 bp
  • A to G, chromosome 14 at 20,719,457 bp
  • A to G, chromosome 14 at 34,071,689 bp
  • G to A, chromosome 14 at 47,258,584 bp
  • T to C, chromosome 14 at 57,629,503 bp
  • T to C, chromosome 14 at 77,091,663 bp
  • T to A, chromosome 15 at 10,319,257 bp
  • A to G, chromosome 15 at 10,461,935 bp
  • G to A, chromosome 15 at 10,537,566 bp
  • A to G, chromosome 15 at 66,613,281 bp
  • T to A, chromosome 15 at 76,725,146 bp
  • T to C, chromosome 15 at 101,947,264 bp
  • T to C, chromosome 16 at 12,760,094 bp
  • T to A, chromosome 16 at 20,660,106 bp
  • T to C, chromosome 16 at 30,349,825 bp
  • T to C, chromosome 16 at 58,824,346 bp
  • T to C, chromosome 16 at 58,889,965 bp
  • A to G, chromosome 16 at 91,643,040 bp
  • T to A, chromosome 17 at 20,227,835 bp
  • G to A, chromosome 17 at 35,078,146 bp
  • A to G, chromosome 17 at 36,774,999 bp
  • A to G, chromosome 17 at 55,778,872 bp
  • A to T, chromosome 17 at 67,750,553 bp
  • T to A, chromosome 17 at 74,660,614 bp
  • T to C, chromosome 18 at 65,306,616 bp
  • A to G, chromosome 19 at 11,454,697 bp
  • T to G, chromosome 19 at 16,759,671 bp
  • T to A, chromosome 19 at 58,744,085 bp
  • A to G, chromosome X at 152,047,975 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2421 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040383-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.