Strain Name:
C57BL/6J-MtgxR2427Btlr/Mmmh
Stock Number:
040389-MU
Citation ID:
RRID:MMRRC_040389-MU
Other Names:
R2427 (G1), C57BL/6J-MtgxR2427Btlr
Major Collection:

Strain Information

Tyrp1
Name: tyrosinase-related protein 1
Synonyms: TRP1, TRP-1, Oca3, isa, Tyrp
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22178
Homologene: 464
Chrnb4
Name: cholinergic receptor, nicotinic, beta polypeptide 4
Synonyms: Acrb4, Acrb-4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 108015
VEGA: 9
HGNC: HGNC:1964
Homologene: 20196
Upf1
Name: UPF1 RNA helicase and ATPase
Synonyms: Rent1, B430202H16Rik, PNORF-1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19704
HGNC: HGNC:9962
Homologene: 2185
Zfand6
Name: zinc finger, AN1-type domain 6
Synonyms: 3110005P07Rik, Za20d3, Awp1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 65098
Homologene: 10372
Plxnd1
Name: plexin D1
Synonyms: 6230425C21Rik, b2b1863Clo, b2b553Clo
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67784
HGNC: HGNC:9107
Homologene: 22866
Axin2
Name: axin 2
Synonyms: Conductin, Axil
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12006
HGNC: HGNC:904
Homologene: 3420
Frg1
Name: FSHD region gene 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 14300
HGNC: HGNC:3954
Homologene: 3295
Mef2a
Name: myocyte enhancer factor 2A
Synonyms: A430079H05Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17258
HGNC: HGNC:6993
Homologene: 4080
Igsf9
Name: immunoglobulin superfamily, member 9
Synonyms: Dasm1, NRT1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 93842
Homologene: 10815
Fmnl2
Name: formin-like 2
Synonyms: 5430425K04Rik, man
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71409
Homologene: 70871
Cep295
Name: centrosomal protein 295
Synonyms: LOC382128, 5830418K08Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 319675
Homologene: 27936
Cldn4
Name: claudin 4
Synonyms: Cpetr1, Cpetr
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12740
HGNC: HGNC:2046
Homologene: 1000
Fat2
Name: FAT atypical cadherin 2
Synonyms: EMI2, mKIAA0811, LOC245827, Fath2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Zfp648
Name: zinc finger protein 648
Synonyms: LOC207678, Gm10178
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 100503355
Homologene: 18996
Slx4
Name: SLX4 structure-specific endonuclease subunit homolog (S. cerevisiae)
Synonyms: D16Bwg1016e, Btbd12
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 52864
Homologene: 23770
Ccdc180
Name: coiled-coil domain containing 180
Synonyms: E230008N13Rik, LOC381522
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381522
Homologene: 117988
Atp2a1
Name: ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
Synonyms: SERCA1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11937
HGNC: HGNC:811
Homologene: 7635
Ctns
Name: cystinosis, nephropathic
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 83429
HGNC: HGNC:2518
Homologene: 3625
I830077J02Rik
Name: RIKEN cDNA I830077J02 gene
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 433638
Homologene: 45482
Capn13
Name: calpain 13
Synonyms: LOC381122
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 381122
VEGA: 17
Homologene: 27026
Slco6b1
Name: solute carrier organic anion transporter family, member 6b1
Synonyms: 1700022M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67854
Homologene: 12199
CT025661.1
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Lrrc4b
Name: leucine rich repeat containing 4B
Synonyms: Lrig4, NGL-3, Ngl3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 272381
Homologene: 45681
Eme1
Name: essential meiotic structure-specific endonuclease 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268465
Homologene: 16123
Tgm1
Name: transglutaminase 1, K polypeptide
Synonyms: TG K, TGase 1, K polypeptide, 2310004J08Rik, protein-glutamine-gamma-glutamyltransferase, TGase1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 21816
VEGA: 14
Homologene: 306
Gnat2
Name: G protein subunit alpha transducin 2
Synonyms: Tcalpha, Gt-2, Gnat-2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14686
HGNC: HGNC:4394
Homologene: 21092
Ankmy1
Name: ankyrin repeat and MYND domain containing 1
Synonyms: 4930483I10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241158
Homologene: 9561
Klra10
Name: killer cell lectin-like receptor subfamily A, member 10
Synonyms: Ly49J, Ly49i2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16628
Homologene: 110821
Lrrc71
Name: leucine rich repeat containing 71
Synonyms: 4933430H15Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74485
Homologene: 83646
Cers3
Name: ceramide synthase 3
Synonyms: T3L, 4930550L11Rik, Lass3, CerS3, LOC233330, related to TRH3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 545975
Homologene: 18719
Crbn
Name: cereblon
Synonyms: 2900045O07Rik, 2610203G15Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 58799
Homologene: 9461
Nt5el
Name: 5' nucleotidase, ecto-like
Synonyms: 4933425L06Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 66763
Homologene: 12028
Ly9
Name: lymphocyte antigen 9
Synonyms: SLAMF3, CD229, Lgp100, T100
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17085
HGNC: HGNC:6730
Homologene: 1759
Rab27b
Name: RAB27B, member RAS oncogene family
Synonyms: B130064M09Rik, 2310021G14Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 80718
HGNC: HGNC:9767
Homologene: 20879
Rasa4
Name: RAS p21 protein activator 4
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 54153
Homologene: 5080
Tpm2
Name: tropomyosin 2, beta
Synonyms: Trop-2, Tpm-2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22004
Homologene: 134309
Tafa4
Name: TAFA chemokine like family member 4
Synonyms: Tafa4, C130034I18Rik, Fam19a4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320701
Homologene: 18561
Ciao1
Name: cytosolic iron-sulfur protein assembly 1
Synonyms: Wdr39
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 26371
Homologene: 55850
AC162906.2
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 92,870,807 bp
  • A to G, chromosome 1 at 96,987,802 bp
  • G to A, chromosome 1 at 154,205,073 bp
  • A to T, chromosome 1 at 171,607,232 bp
  • A to G, chromosome 1 at 172,490,739 bp
  • A to G, chromosome 2 at 53,116,979 bp
  • T to C, chromosome 2 at 127,246,691 bp
  • T to C, chromosome 3 at 87,746,002 bp
  • G to T, chromosome 3 at 105,928,004 bp
  • T to C, chromosome 3 at 108,095,674 bp
  • T to C, chromosome 4 at 43,523,306 bp
  • A to G, chromosome 4 at 45,929,545 bp
  • A to G, chromosome 4 at 80,850,871 bp
  • A to T, chromosome 5 at 134,946,477 bp
  • A to G, chromosome 5 at 136,102,027 bp
  • C to T, chromosome 6 at 97,014,367 bp
  • T to C, chromosome 6 at 106,783,472 bp
  • C to T, chromosome 6 at 115,967,748 bp
  • T to A, chromosome 6 at 130,279,335 bp
  • T to A, chromosome 7 at 44,462,552 bp
  • T to C, chromosome 7 at 66,795,793 bp
  • A to G, chromosome 7 at 67,266,060 bp
  • T to A, chromosome 7 at 84,634,290 bp
  • T to A, chromosome 7 at 126,446,583 bp
  • T to C, chromosome 8 at 41,414,866 bp
  • T to C, chromosome 8 at 70,333,988 bp
  • A to G, chromosome 9 at 15,334,238 bp
  • T to C, chromosome 9 at 55,034,817 bp
  • T to C, chromosome 9 at 109,652,860 bp
  • T to A, chromosome 11 at 55,310,812 bp
  • A to G, chromosome 11 at 73,196,686 bp
  • G to A, chromosome 11 at 94,650,975 bp
  • T to A, chromosome 11 at 108,923,974 bp
  • C to T, chromosome 12 at 114,724,072 bp
  • T to C, chromosome 13 at 105,109,761 bp
  • C to T, chromosome 14 at 55,712,100 bp
  • G to A, chromosome 16 at 3,988,987 bp
  • A to T, chromosome 17 at 73,326,317 bp
  • T to C, chromosome 18 at 69,996,134 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2427 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040389-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.