Strain Name:
C57BL/6J-MtgxR2433Btlr/Mmmh
Stock Number:
040394-MU
Citation ID:
RRID:MMRRC_040394-MU
Other Names:
R2433 (G1), C57BL/6J-MtgxR2433Btlr
Major Collection:

Strain Information

Spns1
Name: spinster homolog 1
Synonyms: 2210013K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73658
Homologene: 41530
Hmgcr
Name: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Synonyms: HMG-CoAR, 3-hydroxy-3-methylglutaryl-CoA reductase, Red
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 15357
HGNC: HGNC:5006
Homologene: 30994
Trip12
Name: thyroid hormone receptor interactor 12
Synonyms: Gtl6, 1110036I07Rik, 6720416K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14897
Homologene: 44226
Slc12a3
Name: solute carrier family 12, member 3
Synonyms: NCC, TSC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20497
Homologene: 287
Ddx39a
Name: DEAD box helicase 39a
Synonyms: 2610307C23Rik, BAT1, Ddx39
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 68278
Homologene: 68487
Slc7a6os
Name: solute carrier family 7, member 6 opposite strand
Synonyms: 2010007L18Rik, 2400002F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66432
Homologene: 41853
Robo1
Name: roundabout guidance receptor 1
Synonyms: DUTT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 19876
Homologene: 2206
Fam120a
Name: family with sequence similarity 120, member A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218236
VEGA: 13
Homologene: 8752
Htt
Name: huntingtin
Synonyms: huntingtin, HD, htt, IT15, C430023I11Rik, Hdh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 15194
HGNC: HGNC:4851
Homologene: 1593
Eipr1
Name: EARP complex and GARP complex interacting protein 1
Synonyms: D12Ertd604e, Tssc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 380752
VEGA: 12
Homologene: 2481
Smurf2
Name: SMAD specific E3 ubiquitin protein ligase 2
Synonyms: 2810411E22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66313
Homologene: 41490
Ttll5
Name: tubulin tyrosine ligase-like family, member 5
Synonyms: 4930556H18Rik, 1700048H13Rik, 2310009M18Rik, D630041K24Rik, STAMP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 320244
Homologene: 9013
Atp6v0a4
Name: ATPase, H+ transporting, lysosomal V0 subunit A4
Synonyms: V-ATPase alpha 4, Atp6n1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 140494
HGNC: HGNC:866
Homologene: 39904
Ugcg
Name: UDP-glucose ceramide glucosyltransferase
Synonyms: GlcT-1, Epcs21, Ugcgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22234
Homologene: 37763
Dhrs11
Name: dehydrogenase/reductase (SDR family) member 11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 192970
Homologene: 41560
Ulk4
Name: unc-51-like kinase 4
Synonyms: 4932415A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 209012
Homologene: 41205
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Lrp4
Name: low density lipoprotein receptor-related protein 4
Synonyms: 6430526J12Rik, Megf7, mdig
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228357
HGNC: HGNC:6696
Homologene: 17964
Sema4f
Name: sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain
Synonyms: Sema W
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20355
Homologene: 3147
Myo5b
Name: myosin VB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 17919
HGNC: HGNC:7603
Homologene: 49481
St8sia3
Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3
Synonyms: ST8SiaIII, Siat8c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 20451
Homologene: 8285
Phlpp2
Name: PH domain and leucine rich repeat protein phosphatase 2
Synonyms: C130044A18Rik, Phlppl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244650
Homologene: 71015
Nrxn3
Name: neurexin III
Synonyms: neurexin III beta, neurexin III alpha, neurexin III beta, neurexin III alpha, 9330112C09Rik, D12Bwg0831e, 4933401A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18191
HGNC: HGNC:8010
Homologene: 83225
Bpifb4
Name: BPI fold containing family B, member 4
Synonyms: LOC381399, Gm1006
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 381399
Homologene: 66971
Zfp944
Name: zinc finger protein 944
Synonyms: 6330416L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 319615
VEGA: 17
Homologene: 133237
Grid2
Name: glutamate receptor, ionotropic, delta 2
Synonyms: GluRdelta2, tpr, B230104L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14804
HGNC: HGNC:4576
Homologene: 74399
Sdcbp
Name: syndecan binding protein
Synonyms: syntenin, syndecan interacting protein, Sycl, MDA-9, syntenin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 53378
Homologene: 4110
Pde3b
Name: phosphodiesterase 3B, cGMP-inhibited
Synonyms: 9830102A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18576
HGNC: HGNC:8779
Homologene: 709
Tacc3
Name: transforming, acidic coiled-coil containing protein 3
Synonyms: Arnt interacting protein, Aint
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21335
Homologene: 81618
Capn10
Name: calpain 10
Synonyms: Capn8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 23830
HGNC: HGNC:1477
Homologene: 36323
Pttg1
Name: pituitary tumor-transforming gene 1
Synonyms: PTTG, securin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 30939
Homologene: 3110
Trdv2-2
Name: T cell receptor delta variable 2-2
Synonyms: Gm17015
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 320913
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 84,743,823 bp
  • T to A, chromosome 1 at 92,942,525 bp
  • G to A, chromosome 2 at 91,506,015 bp
  • A to G, chromosome 2 at 153,959,677 bp
  • T to C, chromosome 4 at 6,386,525 bp
  • A to G, chromosome 4 at 59,207,876 bp
  • A to G, chromosome 5 at 33,671,739 bp
  • A to T, chromosome 5 at 34,907,541 bp
  • A to G, chromosome 6 at 38,082,029 bp
  • T to C, chromosome 6 at 64,076,773 bp
  • A to T, chromosome 6 at 82,939,509 bp
  • T to C, chromosome 7 at 114,526,837 bp
  • G to A, chromosome 7 at 126,371,165 bp
  • A to G, chromosome 8 at 83,721,723 bp
  • T to C, chromosome 8 at 94,346,316 bp
  • T to C, chromosome 8 at 106,204,371 bp
  • T to G, chromosome 8 at 109,940,002 bp
  • T to C, chromosome 9 at 121,157,033 bp
  • A to T, chromosome 11 at 43,420,351 bp
  • A to T, chromosome 11 at 59,039,086 bp
  • C to A, chromosome 11 at 84,828,919 bp
  • T to A, chromosome 11 at 106,868,664 bp
  • G to A, chromosome 12 at 28,863,043 bp
  • A to T, chromosome 12 at 86,014,757 bp
  • A to G, chromosome 12 at 89,976,392 bp
  • A to C, chromosome 13 at 48,933,968 bp
  • A to G, chromosome 13 at 96,665,885 bp
  • A to G, chromosome 14 at 53,961,052 bp
  • T to C, chromosome 16 at 72,970,239 bp
  • A to T, chromosome 17 at 22,339,212 bp
  • A to T, chromosome 18 at 64,269,716 bp
  • A to G, chromosome 18 at 74,759,087 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2433 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040394-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.