Strain Name:
C57BL/6J-MtgxR2471Btlr/Mmmh
Stock Number:
040403-MU
Citation ID:
RRID:MMRRC_040403-MU
Other Names:
R2471 (G1), C57BL/6J-MtgxR2471Btlr
Major Collection:

Gene Information

Map4k5
Name: mitogen-activated protein kinase kinase kinase kinase 5
Synonyms: MAPKKKK5, GCKR, KHS, 4432415E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 399510
HGNC: HGNC:6867
Homologene: 38199
Mad1l1
Name: MAD1 mitotic arrest deficient 1-like 1
Synonyms: Mad1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 17120
HGNC: HGNC:6762
Homologene: 74500
Slc31a2
Name: solute carrier family 31, member 2
Synonyms: Ctr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 20530
Homologene: 37536
Serpina9
Name: serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 9
Synonyms: Centerin, 2310014L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 71907
Homologene: 23633
Zfp101
Name: zinc finger protein 101
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22643
Homologene: 137226
Ptprg
Name: protein tyrosine phosphatase, receptor type, G
Synonyms: 5430405N12Rik, RPTPgamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 19270
HGNC: HGNC:9671
Homologene: 2129
Tsc22d1
Name: TSC22 domain family, member 1
Synonyms: TSC-22, Egr5, Tgfb1i4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21807
Homologene: 7573
Ipo5
Name: importin 5
Synonyms: RanBP5, 5730478E03Rik, 1110011C18Rik, Kpnb3, IMB3, importin beta 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 70572
VEGA: 14
HGNC: HGNC:6402
Homologene: 1710
Flrt1
Name: fibronectin leucine rich transmembrane protein 1
Synonyms: D630040I23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 396184
VEGA: 19
HGNC: HGNC:3760
Homologene: 40864
Pcdh20
Name: protocadherin 20
Synonyms: C630015B17Rik, PCDH13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219257
Homologene: 11277
Dnajc1
Name: DnaJ heat shock protein family (Hsp40) member C1
Synonyms: MTJ1, Dnajl1, ERdj1, 4733401K02Rik, D230036H06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13418
Homologene: 7293
Marchf7
Name: membrane associated ring-CH-type finger 7
Synonyms: Axot, Gtrgeo17, March7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 57438
Homologene: 10753
Bicra
Name: BRD4 interacting chromatin remodeling complex associated protein
Synonyms: Gltscr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243842
HGNC: HGNC:4332
Homologene: 9250
Mctp2
Name: multiple C2 domains, transmembrane 2
Synonyms: LOC244049
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244049
Homologene: 69254
Sigirr
Name: single immunoglobulin and toll-interleukin 1 receptor (TIR) domain
Synonyms: Sigirr, Tir8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 24058
Homologene: 36399
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 94109
Homologene: 69536
Slc16a12
Name: solute carrier family 16 (monocarboxylic acid transporters), member 12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 240638
Homologene: 130007
F3
Name: coagulation factor III
Synonyms: tissue factor, TF, Cf-3, CD142, Cf3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 14066
HGNC: HGNC:3541
Homologene: 1511
Olfr209
Name: olfactory receptor 209
Synonyms: GA_x54KRFPKG5P-55590495-55589578, MOR182-6, MOR182-6, GA_x54KRFPKG5P-55580232-55579315, Olfr208
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 404311
Homologene: 133040
Taok2
Name: TAO kinase 2
Synonyms: MAP3K17, TAO2, TAO1, PSK1, 1110033K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381921
Homologene: 74531
Plce1
Name: phospholipase C, epsilon 1
Synonyms: 4933403A21Rik, PLCepsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 74055
Homologene: 9478
Smc1b
Name: structural maintenance of chromosomes 1B
Synonyms: SMC1beta, Smc1l2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 140557
VEGA: 15
Homologene: 13786
Cacnb2
Name: calcium channel, voltage-dependent, beta 2 subunit
Synonyms: Cchb2, Cavbeta2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12296
HGNC: HGNC:1402
Homologene: 75191
Emcn
Name: endomucin
Synonyms: 0610012K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 59308
Homologene: 9438
Ofcc1
Name: orofacial cleft 1 candidate 1
Synonyms: ojoplano, Opo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218165
VEGA: 13
Homologene: 125376
Cstl1
Name: cystatin-like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228756
Homologene: 16283
Ssrp1
Name: structure specific recognition protein 1
Synonyms: T160, Hmgi-rs3, Hmg1-rs1, Hmgox
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20833
Homologene: 110735
Olfr1447
Name: olfactory receptor 1447
Synonyms: GA_x6K02T2RE5P-3231251-3230331, MOR202-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258698
Homologene: 74233
Far2
Name: fatty acyl CoA reductase 2
Synonyms: Mlstd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330450
Homologene: 69247
Try10
Name: trypsin 10
Synonyms: trypsinogen 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 436522
HGNC: HGNC:9475
Homologene: 134623
Slc2a12
Name: solute carrier family 2 (facilitated glucose transporter), member 12
Synonyms: Glut12, GLUT-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 353169
Homologene: 59263
Nr1h4
Name: nuclear receptor subfamily 1, group H, member 4
Synonyms: RIP14, FXR, HRR1, Rxrip14, Fxr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 20186
HGNC: HGNC:7967
Homologene: 3760
Olfr1369-ps1
Name: olfactory receptor 1369, pseudogene 1
Synonyms: GA_x6K02T2QHY8-12126170-12125935, MOR256-31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 404504
HGNC: HGNC:8281
Gimap7
Name: GTPase, IMAP family member 7
Synonyms: IAN7, Ian3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 231932
Homologene: 134307
Kcnf1
Name: potassium voltage-gated channel, subfamily F, member 1
Synonyms: LOC382571
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 382571
HGNC: HGNC:6246
Homologene: 124236
Taar9
Name: trace amine-associated receptor 9
Synonyms: Tar3, Ta3, Trar3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 503558
Homologene: 65286
4930563M21Rik
Name: RIKEN cDNA 4930563M21 gene
Synonyms: Rfpl3s
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 75258
VEGA: 9
Homologene: 138467
AC153864.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 2 at 14,984,314 bp
  • T to C, chromosome 2 at 18,219,816 bp
  • A to G, chromosome 2 at 60,236,900 bp
  • A to G, chromosome 2 at 85,042,298 bp
  • T to A, chromosome 2 at 148,751,121 bp
  • G to T, chromosome 3 at 121,725,040 bp
  • C to T, chromosome 3 at 137,404,011 bp
  • A to T, chromosome 4 at 62,296,075 bp
  • T to C, chromosome 5 at 140,261,552 bp
  • T to C, chromosome 6 at 41,356,746 bp
  • G to A, chromosome 6 at 48,724,052 bp
  • A to G, chromosome 6 at 148,138,694 bp
  • A to G, chromosome 7 at 15,972,332 bp
  • T to A, chromosome 7 at 72,161,161 bp
  • T to C, chromosome 7 at 126,875,100 bp
  • A to G, chromosome 7 at 141,092,597 bp
  • T to A, chromosome 8 at 16,211,762 bp
  • A to T, chromosome 9 at 55,980,863 bp
  • A to T, chromosome 10 at 22,664,807 bp
  • A to T, chromosome 10 at 24,109,391 bp
  • A to T, chromosome 10 at 77,690,520 bp
  • A to T, chromosome 10 at 89,473,894 bp
  • T to C, chromosome 12 at 17,175,530 bp
  • T to C, chromosome 12 at 69,856,846 bp
  • A to C, chromosome 12 at 103,997,206 bp
  • A to T, chromosome 13 at 21,116,429 bp
  • G to A, chromosome 13 at 40,097,025 bp
  • T to C, chromosome 14 at 12,210,327 bp
  • A to G, chromosome 14 at 76,418,204 bp
  • T to G, chromosome 14 at 88,467,236 bp
  • A to C, chromosome 14 at 120,922,162 bp
  • T to C, chromosome 15 at 85,092,017 bp
  • A to T, chromosome 16 at 59,361,581 bp
  • T to C, chromosome 17 at 33,380,976 bp
  • A to T, chromosome 19 at 7,096,491 bp
  • G to A, chromosome 19 at 12,901,315 bp
  • C to T, chromosome 19 at 34,677,277 bp
  • C to T, chromosome 19 at 38,779,926 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2471 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040403-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.