Strain Name:
C57BL/6J-MtgxR2484Btlr/Mmmh
Stock Number:
040408-MU
Citation ID:
RRID:MMRRC_040408-MU
Other Names:
R2484 (G1), C57BL/6J-MtgxR2484Btlr
Major Collection:

Gene Information

Gene Symbol: Drd4 [MGI:94926] (Mus musculus (mouse))
Name: dopamine receptor D4
Synonyms: D4R, Drd-4
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Shh [MGI:98297] (Mus musculus (mouse))
Name: sonic hedgehog
Synonyms: M100081, Hxl3, Hhg1, Hx
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Macf1 [MGI:108559] (Mus musculus (mouse))
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Nub1 [MGI:1889001] (Mus musculus (mouse))
Name: negative regulator of ubiquitin-like proteins 1
Synonyms: 4931404D21Rik, NY-REN-18, BS4, 6330412F12Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Nptn [MGI:108077] (Mus musculus (mouse))
Name: neuroplastin
Synonyms: Sdfr1
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Wwp1 [MGI:1861728] (Mus musculus (mouse))
Name: WW domain containing E3 ubiquitin protein ligase 1
Synonyms: AIP5, SDRP1, Tiul1, 8030445B08Rik
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Prdm2 [MGI:107628] (Mus musculus (mouse))
Name: PR domain containing 2, with ZNF domain
Synonyms: E330024L24Rik, LOC381568, Riz1, Riz, KMT8, 4833427P12Rik
Chromosome: 4
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 11,596,302 bp
  • C to T, chromosome 1 at 66,521,581 bp
  • A to T, chromosome 1 at 92,944,843 bp
  • G to T, chromosome 1 at 164,143,954 bp
  • G to T, chromosome 1 at 164,143,955 bp
  • A to T, chromosome 1 at 172,285,800 bp
  • A to T, chromosome 1 at 180,989,972 bp
  • A to G, chromosome 1 at 183,396,084 bp
  • T to C, chromosome 2 at 32,304,770 bp
  • T to C, chromosome 2 at 32,777,171 bp
  • G to A, chromosome 2 at 68,711,475 bp
  • A to G, chromosome 2 at 89,752,234 bp
  • C to G, chromosome 2 at 91,056,001 bp
  • T to C, chromosome 2 at 137,084,700 bp
  • T to A, chromosome 2 at 152,311,363 bp
  • T to G, chromosome 3 at 59,297,838 bp
  • T to A, chromosome 3 at 62,472,815 bp
  • T to C, chromosome 3 at 64,503,971 bp
  • T to C, chromosome 3 at 107,628,221 bp
  • T to C, chromosome 3 at 116,782,654 bp
  • T to C, chromosome 3 at 123,552,537 bp
  • T to C, chromosome 3 at 129,822,391 bp
  • C to G, chromosome 4 at 6,688,886 bp
  • A to C, chromosome 4 at 19,628,019 bp
  • A to C, chromosome 4 at 123,473,672 bp
  • T to A, chromosome 4 at 143,135,206 bp
  • A to G, chromosome 5 at 24,708,702 bp
  • A to G, chromosome 5 at 28,466,742 bp
  • C to T, chromosome 5 at 137,112,674 bp
  • G to C, chromosome 5 at 138,809,117 bp
  • C to A, chromosome 5 at 143,202,093 bp
  • C to T, chromosome 5 at 144,556,345 bp
  • A to G, chromosome 6 at 32,804,015 bp
  • T to C, chromosome 6 at 108,369,110 bp
  • A to G, chromosome 6 at 129,868,757 bp
  • A to T, chromosome 6 at 138,657,494 bp
  • T to C, chromosome 7 at 10,169,131 bp
  • T to A, chromosome 7 at 15,988,680 bp
  • T to C, chromosome 7 at 98,888,461 bp
  • A to G, chromosome 7 at 103,979,338 bp
  • C to T, chromosome 7 at 141,294,736 bp
  • A to G, chromosome 8 at 24,533,815 bp
  • A to G, chromosome 8 at 70,069,524 bp
  • A to G, chromosome 8 at 109,732,251 bp
  • A to G, chromosome 8 at 110,513,115 bp
  • T to C, chromosome 8 at 125,891,120 bp
  • A to G, chromosome 9 at 58,643,673 bp
  • A to G, chromosome 9 at 64,986,084 bp
  • T to A, chromosome 9 at 75,445,473 bp
  • T to C, chromosome 9 at 105,780,804 bp
  • G to A, chromosome 9 at 120,424,619 bp
  • T to C, chromosome 10 at 19,601,415 bp
  • T to C, chromosome 10 at 127,875,077 bp
  • T to A, chromosome 11 at 54,642,756 bp
  • T to A, chromosome 11 at 59,007,540 bp
  • T to C, chromosome 11 at 73,689,682 bp
  • A to T, chromosome 11 at 76,239,518 bp
  • A to T, chromosome 11 at 83,861,835 bp
  • C to A, chromosome 12 at 16,547,499 bp
  • C to T, chromosome 12 at 30,103,969 bp
  • T to C, chromosome 12 at 110,933,318 bp
  • A to T, chromosome 13 at 8,569,774 bp
  • G to T, chromosome 14 at 54,961,242 bp
  • A to T, chromosome 14 at 65,075,735 bp
  • A to T, chromosome 14 at 73,581,709 bp
  • A to T, chromosome 15 at 5,235,173 bp
  • T to C, chromosome 15 at 8,323,698 bp
  • A to T, chromosome 15 at 90,980,785 bp
  • C to T, chromosome 16 at 44,220,682 bp
  • A to T, chromosome 17 at 24,511,639 bp
  • T to A, chromosome 17 at 33,588,198 bp
  • C to A, chromosome 17 at 56,614,659 bp
  • A to G, chromosome 17 at 56,882,641 bp
  • A to G, chromosome 17 at 83,222,238 bp
  • G to A, chromosome 18 at 60,916,651 bp
  • A to G, chromosome 18 at 82,668,637 bp
  • C to T, chromosome 19 at 12,901,641 bp
  • C to T, chromosome 19 at 37,903,843 bp
  • A to C, chromosome 19 at 55,380,979 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2484 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040408-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.