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Strain Name:
C57BL/6J-MtgxR2520Btlr/Mmmh
Stock Number:
040424-MU
Citation ID:
RRID:MMRRC_040424-MU
Other Names:
R2520 (G1), C57BL/6J-MtgxR2520Btlr
Major Collection:

Strain Information

Ntrk2
Name: neurotrophic tyrosine kinase, receptor, type 2
Synonyms: trkB, C030027L06Rik, Tkrb
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18212
VEGA: 13
HGNC: HGNC:8032
Homologene: 4504
Nhlh1
Name: nescient helix loop helix 1
Synonyms: Hen1, Nscl, Tal2, Nscl, bHLHa35
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18071
HGNC: HGNC:7817
Homologene: 4089
Gria2
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: GluR-B, GluR2, Glur-2, Glur2, GluA2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14800
HGNC: HGNC:4572
Homologene: 20225
Nudt2
Name: nudix hydrolase 2
Synonyms: APAH1, 2310051L06Rik, nudix (nucleoside diphosphate linked moiety X)-type motif 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66401
HGNC: HGNC:8049
Homologene: 896
Zbtb8a
Name: zinc finger and BTB domain containing 8a
Synonyms: 2410081M15Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 73680
Homologene: 12506
Epop
Name: elongin BC and polycomb repressive complex 2 associated protein
Synonyms: E13, E130012A19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103551
Homologene: 82351
Lyz2
Name: lysozyme 2
Synonyms: Lzp, Lzm, Lys, Lzm-s1, Lyzs, Lysm
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17105
VEGA: 10
HGNC: HGNC:6740
Homologene: 121490
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 17,750,776 bp
  • T to A, chromosome 1 at 40,471,823 bp
  • T to A, chromosome 1 at 150,743,647 bp
  • T to A, chromosome 1 at 151,453,945 bp
  • G to T, chromosome 1 at 172,054,003 bp
  • G to A, chromosome 2 at 26,441,356 bp
  • GCCACCA to GCCA, chromosome 2 at 52,110,324 bp
  • A to T, chromosome 2 at 112,071,668 bp
  • A to G, chromosome 2 at 181,589,502 bp
  • C to T, chromosome 3 at 5,403,358 bp
  • A to T, chromosome 3 at 20,029,030 bp
  • A to C, chromosome 3 at 80,706,962 bp
  • T to A, chromosome 3 at 114,151,442 bp
  • G to A, chromosome 3 at 146,650,506 bp
  • A to G, chromosome 3 at 154,962,105 bp
  • T to A, chromosome 4 at 11,803,866 bp
  • A to G, chromosome 4 at 41,480,354 bp
  • T to A, chromosome 4 at 48,604,679 bp
  • A to T, chromosome 4 at 82,950,290 bp
  • A to T, chromosome 4 at 126,035,352 bp
  • A to G, chromosome 4 at 129,359,896 bp
  • A to G, chromosome 4 at 137,625,251 bp
  • A to T, chromosome 5 at 3,575,773 bp
  • T to C, chromosome 5 at 24,712,575 bp
  • T to C, chromosome 5 at 33,358,510 bp
  • C to T, chromosome 5 at 124,080,028 bp
  • T to C, chromosome 5 at 124,129,401 bp
  • G to A, chromosome 5 at 142,085,771 bp
  • C to A, chromosome 6 at 85,667,963 bp
  • T to A, chromosome 6 at 122,713,459 bp
  • T to A, chromosome 6 at 123,200,983 bp
  • A to C, chromosome 7 at 25,641,699 bp
  • A to T, chromosome 7 at 47,335,272 bp
  • T to C, chromosome 7 at 80,250,216 bp
  • C to T, chromosome 7 at 83,651,994 bp
  • T to C, chromosome 7 at 100,261,593 bp
  • A to G, chromosome 7 at 104,331,239 bp
  • T to A, chromosome 7 at 104,892,873 bp
  • A to G, chromosome 7 at 127,200,283 bp
  • A to G, chromosome 7 at 127,993,505 bp
  • A to G, chromosome 8 at 22,471,434 bp
  • A to G, chromosome 8 at 72,739,718 bp
  • T to A, chromosome 8 at 126,394,573 bp
  • G to A, chromosome 9 at 21,583,597 bp
  • A to T, chromosome 9 at 41,014,947 bp
  • A to G, chromosome 9 at 51,943,663 bp
  • A to T, chromosome 9 at 75,297,649 bp
  • G to T, chromosome 9 at 103,489,282 bp
  • A to G, chromosome 9 at 114,689,006 bp
  • G to A, chromosome 10 at 7,907,481 bp
  • A to G, chromosome 10 at 52,319,545 bp
  • T to C, chromosome 10 at 71,381,217 bp
  • T to A, chromosome 10 at 80,233,055 bp
  • T to A, chromosome 10 at 117,278,653 bp
  • T to C, chromosome 11 at 29,791,993 bp
  • A to G, chromosome 11 at 75,906,547 bp
  • A to G, chromosome 11 at 97,628,728 bp
  • G to A, chromosome 11 at 105,348,431 bp
  • A to T, chromosome 11 at 120,580,026 bp
  • T to C, chromosome 12 at 57,238,583 bp
  • A to T, chromosome 13 at 21,142,576 bp
  • T to A, chromosome 13 at 59,054,276 bp
  • G to T, chromosome 13 at 67,546,662 bp
  • T to A, chromosome 13 at 77,051,265 bp
  • A to T, chromosome 13 at 93,940,699 bp
  • A to G, chromosome 13 at 105,445,373 bp
  • T to A, chromosome 14 at 5,863,642 bp
  • A to T, chromosome 14 at 77,582,399 bp
  • A to T, chromosome 15 at 39,827,019 bp
  • G to T, chromosome 15 at 82,330,094 bp
  • C to T, chromosome 15 at 96,361,991 bp
  • G to A, chromosome 15 at 99,736,381 bp
  • T to G, chromosome 16 at 20,663,076 bp
  • T to A, chromosome 16 at 32,278,403 bp
  • A to T, chromosome 16 at 88,778,526 bp
  • G to A, chromosome 17 at 25,855,352 bp
  • G to A, chromosome 17 at 28,163,865 bp
  • G to A, chromosome 17 at 33,916,982 bp
  • G to A, chromosome 17 at 49,480,757 bp
  • T to C, chromosome 17 at 56,206,868 bp
  • A to G, chromosome 17 at 80,216,370 bp
  • A to T, chromosome 18 at 54,988,506 bp
  • T to A, chromosome 19 at 36,612,233 bp
  • T to A, chromosome 19 at 46,265,367 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2520 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040424-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.