Strain Name:
C57BL/6J-MtgxR2846Btlr/Mmmh
Stock Number:
040439-MU
Citation ID:
RRID:MMRRC_040439-MU
Other Names:
R2846 (G1), C57BL/6J-MtgxR2846Btlr
Major Collection:

Gene Information

Rfx3
Name: regulatory factor X, 3 (influences HLA class II expression)
Synonyms: MRFX3, C230093O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 19726
HGNC: HGNC:9984
Homologene: 7917
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: Dlgh1, B130052P05Rik, SAP97
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Plekha1
Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
Synonyms: TAPP1, C920009D07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101476
Homologene: 11001
Mark2
Name: MAP/microtubule affinity regulating kinase 2
Synonyms: Par-1, Emk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 13728
HGNC: HGNC:3332
Homologene: 69013
Sec24d
Name: Sec24 related gene family, member D (S. cerevisiae)
Synonyms: LOC383951, 2310020L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 69608
Homologene: 40986
Ddx4
Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Synonyms: Mvh, VASA, mvh / m'vasa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 13206
VEGA: 13
Homologene: 49227
Prr12
Name: proline rich 12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233210
Homologene: 18957
Dtna
Name: dystrobrevin alpha
Synonyms: Dtn, 2210407P21Rik, a-DB-1, alpha-dystrobrevin, A0, 87K protein, adbn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13527
VEGA: 18
HGNC: HGNC:3057
Homologene: 20362
Orc3
Name: origin recognition complex, subunit 3
Synonyms: Orc3l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 50793
HGNC: HGNC:8489
Homologene: 8225
Irs4
Name: insulin receptor substrate 4
Synonyms: IRS-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 16370
HGNC: HGNC:6128
Homologene: 137235
Kremen1
Name: kringle containing transmembrane protein 1
Synonyms: Krm1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 84035
Homologene: 12935
Pdgfrb
Name: platelet derived growth factor receptor, beta polypeptide
Synonyms: Pdgfr, CD140b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 18596
HGNC: HGNC:8804
Homologene: 1960
Smarcb1
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
Synonyms: Ini1, Baf47, Snf5, integrase interactor 1, SNF5/INI1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 20587
Homologene: 2310
Zbtb8os
Name: zinc finger and BTB domain containing 8 opposite strand
Synonyms: Arch, 2010001H09Rik, 2310028N13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67106
Homologene: 12083
Slc15a4
Name: solute carrier family 15, member 4
Synonyms: PHT1, C130069N12Rik, PTR4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100561
Homologene: 26432
Tmem204
Name: transmembrane protein 204
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 407831
Homologene: 36419
Zmiz1
Name: zinc finger, MIZ-type containing 1
Synonyms: Zimp10, Rai17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 328365
Homologene: 10667
Tas2r124
Name: taste receptor, type 2, member 124
Synonyms: T2R24, mGR24, Tas2r24, mt2r50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387351
Homologene: 87293
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: PKHDL1, fibrocystin L, D86 mRNA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 192190
Homologene: 16332
Vmn2r6
Name: vomeronasal 2, receptor 6
Synonyms: EG620718, EG667069
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 667069
Homologene: 129754
Fhad1
Name: forkhead-associated (FHA) phosphopeptide binding domain 1
Synonyms: 2900090M10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329977
Homologene: 77947
Ccdc88b
Name: coiled-coil domain containing 88B
Synonyms: 2610041P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 78317
VEGA: 19
Homologene: 49992
Unc79
Name: unc-79 homolog
Synonyms: Mlca3, 9030205A07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217843
Homologene: 41397
Kif21a
Name: kinesin family member 21A
Synonyms: N-5 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16564
VEGA: 15
Homologene: 56761
Mfsd13a
Name: major facilitator superfamily domain containing 13a
Synonyms: 4930538D17Rik, 4930449A08Rik, Tmem180
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 75146
Homologene: 12592
Shank2
Name: SH3 and multiple ankyrin repeat domains 2
Synonyms: ProSAP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 210274
Homologene: 105965
Pign
Name: phosphatidylinositol glycan anchor biosynthesis, class N
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 27392
HGNC: HGNC:8967
Homologene: 6330
St18
Name: suppression of tumorigenicity 18
Synonyms: Nzf3, Myt3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240690
Homologene: 8792
Olfr119
Name: olfactory receptor 119
Synonyms: GA_x6K02T2PSCP-2159633-2160598, MOR263-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258095
Homologene: 17195
Pla2r1
Name: phospholipase A2 receptor 1
Synonyms: Pla2g1br, PLA2-I receptor, M-type receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18779
HGNC: HGNC:9042
Homologene: 32016
Gal3st2c
Name: galactose-3-O-sulfotransferase 2C
Synonyms: Gm6086
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 619597
Homologene: 41471
Ppfia3
Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3
Synonyms: 2410127E16Rik, Liprin-alpha3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 76787
HGNC: HGNC:9247
Homologene: 37833
Hsd3b1
Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
Synonyms: D3Ertd383e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 15492
Homologene: 69149
Qpct
Name: glutaminyl-peptide cyclotransferase (glutaminyl cyclase)
Synonyms: 5730422A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 70536
VEGA: 17
HGNC: HGNC:9753
Homologene: 8238
Mindy4
Name: MINDY lysine 48 deubiquitinase 4
Synonyms: LOC384387, C330043M08Rik, Fam188b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330323
Homologene: 49991
Ssh3
Name: slingshot protein phosphatase 3
Synonyms: SSH-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 245857
VEGA: 19
Homologene: 32372
Tgfbr3l
Name: transforming growth factor, beta receptor III-like
Synonyms: Gm14378, LOC100039590
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 100044509
Homologene: 130375
Olfr384
Name: olfactory receptor 384
Synonyms: GA_x6K02T2P1NL-3773152-3774090, MOR135-5, Olfr386, GA_x6K02T2P1NL-3739520-3740032
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 193053
Homologene: 105206
Vmn1r212
Name: vomeronasal 1 receptor 212
Synonyms: V1rh18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 171275
Homologene: 110880
Bahd1
Name: bromo adjacent homology domain containing 1
Synonyms: LOC228536
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228536
Homologene: 8976
Cdnf
Name: cerebral dopamine neurotrophic factor
Synonyms: Armetl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227526
Homologene: 27965
Gimap4
Name: GTPase, IMAP family member 4
Synonyms: E430007K16Rik, Ian1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 107526
Homologene: 75084
Atg4a
Name: autophagy related 4A, cysteine peptidase
Synonyms: Autl2, Apg4a, autophagin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 666468
Homologene: 70873
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 6,845,587 bp
  • A to T, chromosome 1 at 93,996,400 bp
  • C to A, chromosome 1 at 105,657,796 bp
  • T to A, chromosome 2 at 3,513,128 bp
  • A to C, chromosome 2 at 60,522,943 bp
  • G to A, chromosome 2 at 118,922,523 bp
  • A to G, chromosome 3 at 64,556,790 bp
  • T to C, chromosome 3 at 98,852,778 bp
  • A to G, chromosome 3 at 123,350,746 bp
  • A to T, chromosome 4 at 34,576,341 bp
  • A to T, chromosome 4 at 129,341,516 bp
  • G to T, chromosome 4 at 141,904,968 bp
  • A to G, chromosome 5 at 127,604,536 bp
  • A to G, chromosome 6 at 48,691,352 bp
  • T to C, chromosome 6 at 55,278,100 bp
  • A to G, chromosome 6 at 132,755,267 bp
  • G to C, chromosome 7 at 45,046,012 bp
  • C to A, chromosome 7 at 45,356,428 bp
  • G to T, chromosome 7 at 130,908,365 bp
  • A to G, chromosome 7 at 144,070,055 bp
  • A to G, chromosome 8 at 4,249,280 bp
  • C to A, chromosome 10 at 75,897,541 bp
  • GG to GGGCG, chromosome 11 at 5,201,793 bp
  • A to T, chromosome 11 at 73,603,383 bp
  • T to C, chromosome 12 at 103,042,100 bp
  • A to G, chromosome 13 at 22,884,092 bp
  • T to A, chromosome 13 at 112,604,612 bp
  • A to G, chromosome 14 at 25,645,675 bp
  • T to C, chromosome 15 at 44,539,637 bp
  • T to C, chromosome 15 at 90,934,464 bp
  • T to C, chromosome 16 at 31,863,197 bp
  • G to A, chromosome 17 at 25,080,333 bp
  • T to C, chromosome 17 at 37,700,823 bp
  • A to G, chromosome 17 at 79,070,742 bp
  • T to A, chromosome 18 at 23,651,503 bp
  • C to T, chromosome 18 at 61,064,016 bp
  • T to C, chromosome 19 at 4,265,296 bp
  • T to G, chromosome 19 at 6,854,465 bp
  • T to C, chromosome 19 at 7,286,862 bp
  • T to C, chromosome 19 at 27,806,786 bp
  • C to T, chromosome 19 at 46,371,992 bp
  • G to A, chromosome X at 140,992,840 bp
  • C to A, chromosome X at 141,724,340 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2846 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040439-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.