Strain Name:
C57BL/6J-MtgxR2891Btlr/Mmmh
Stock Number:
040479-MU
Citation ID:
RRID:MMRRC_040479-MU
Other Names:
R2891 (G1), C57BL/6J-MtgxR2891Btlr
Major Collection:

Gene Information

Shprh
Name: SNF2 histone linker PHD RING helicase
Synonyms: 2610103K11Rik, D230017O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 268281
Homologene: 6489
Pik3r1
Name: phosphoinositide-3-kinase regulatory subunit 1
Synonyms: p85alpha, p50alpha, p55alpha, PI3K
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18708
HGNC: HGNC:8979
Homologene: 7889
Adgrl3
Name: adhesion G protein-coupled receptor L3
Synonyms: lectomedin 3, LEC3, D130075K09Rik, 5430402I23Rik, Lphn3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 319387
Homologene: 22878
Vpreb3
Name: pre-B lymphocyte gene 3
Synonyms: Vpreb-3, 8HS-20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22364
Homologene: 7598
Clcn6
Name: chloride channel, voltage-sensitive 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 26372
HGNC: HGNC:2024
Homologene: 985
Ireb2
Name: iron responsive element binding protein 2
Synonyms: Irp2, D9Ertd85e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 64602
VEGA: 9
HGNC: HGNC:6115
Homologene: 11280
Odf2
Name: outer dense fiber of sperm tails 2
Synonyms: MMTEST29, cenexin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18286
HGNC: HGNC:8114
Homologene: 1907
Rapsn
Name: receptor-associated protein of the synapse
Synonyms: rapsyn, Raps, 43kDa acetylcholine receptor-associated protein, Nraps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19400
HGNC: HGNC:9863
Homologene: 3708
Drg2
Name: developmentally regulated GTP binding protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 13495
HGNC: HGNC:3030
Homologene: 1061
Kcnj3
Name: potassium inwardly-rectifying channel, subfamily J, member 3
Synonyms: Kir3.1, GIRK1, Kcnf3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16519
HGNC: HGNC:6264
Homologene: 1687
Nadk
Name: NAD kinase
Synonyms: 4432404C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 192185
Homologene: 49724
Pik3ap1
Name: phosphoinositide-3-kinase adaptor protein 1
Synonyms: BCAP, 1810044J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 83490
VEGA: 19
Homologene: 12848
Klf7
Name: Kruppel-like factor 7 (ubiquitous)
Synonyms: 9830124P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 93691
HGNC: HGNC:6350
Homologene: 2751
Ncoa6
Name: nuclear receptor coactivator 6
Synonyms: PRIP, ASC2, RAP250, AIB3, NRC, ASC-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56406
Homologene: 137337
Per2
Name: period circadian clock 2
Synonyms: mPer2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18627
HGNC: HGNC:8846
Homologene: 7885
Farp1
Name: FERM, RhoGEF (Arhgef) and pleckstrin domain protein 1 (chondrocyte-derived)
Synonyms: Cdep
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 223254
HGNC: HGNC:3591
Homologene: 38098
Grik1
Name: glutamate receptor, ionotropic, kainate 1
Synonyms: Glur-5, Glur5, D16Ium24, A830007B11Rik, D16Ium24e, Glurbeta1, GluK5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 14805
HGNC: HGNC:4579
Homologene: 68992
Pcnx2
Name: pecanex homolog 2
Synonyms: E330039K12Rik, Pcnxl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270109
HGNC: HGNC:8736
Homologene: 8987
Kdr
Name: kinase insert domain protein receptor
Synonyms: VEGF receptor-2, VEGFR-2, vascular endothelial growth factor receptor- 2, VEGFR2, Flk-1, Flk1, orv
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16542
HGNC: HGNC:6307
Homologene: 55639
Klhl9
Name: kelch-like 9
Synonyms: C530050O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242521
Homologene: 23125
P3h2
Name: prolyl 3-hydroxylase 2
Synonyms: Leprel1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 210530
Homologene: 10062
Nt5dc3
Name: 5'-nucleotidase domain containing 3
Synonyms: Gnn, C630002B14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 103466
VEGA: 10
Homologene: 9571
Hhat
Name: hedgehog acyltransferase
Synonyms: Skn, 2810432O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226861
Homologene: 41232
Plod3
Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Synonyms: lysyl hydroxylase 3, LH3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 26433
HGNC: HGNC:9083
Homologene: 843
Taar8a
Name: trace amine-associated receptor 8A
Synonyms: LOC215859
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215859
Homologene: 77586
C2cd6
Name: C2 calcium dependent domain containing 6
Synonyms: 1700052H20Rik, 4930408G06Rik, Als2cr11, Als2cr11b, Gm33589, C2cd6b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 102636554
Homologene: 134310
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 58,997,379 bp
  • T to C, chromosome 1 at 64,042,362 bp
  • C to A, chromosome 1 at 91,445,603 bp
  • T to C, chromosome 1 at 192,595,086 bp
  • A to G, chromosome 2 at 29,925,925 bp
  • A to T, chromosome 2 at 55,447,015 bp
  • A to T, chromosome 2 at 91,036,824 bp
  • T to A, chromosome 2 at 155,437,961 bp
  • T to C, chromosome 4 at 88,720,970 bp
  • C to T, chromosome 4 at 148,012,616 bp
  • A to G, chromosome 4 at 155,587,360 bp
  • A to G, chromosome 5 at 75,946,836 bp
  • T to C, chromosome 5 at 81,693,519 bp
  • G to C, chromosome 5 at 136,988,146 bp
  • A to G, chromosome 8 at 125,891,058 bp
  • A to T, chromosome 9 at 54,899,990 bp
  • T to G, chromosome 10 at 11,164,356 bp
  • G to A, chromosome 10 at 24,077,130 bp
  • C to T, chromosome 10 at 75,943,222 bp
  • T to C, chromosome 10 at 86,804,775 bp
  • T to C, chromosome 11 at 60,461,505 bp
  • T to C, chromosome 13 at 101,701,699 bp
  • C to T, chromosome 14 at 121,256,736 bp
  • T to A, chromosome 16 at 25,981,235 bp
  • T to C, chromosome 16 at 87,946,772 bp
  • G to A, chromosome 19 at 41,376,061 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2891 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040479-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.