Strain Name:
C57BL/6J-MtgxR2896Btlr/Mmmh
Stock Number:
040484-MU
Citation ID:
RRID:MMRRC_040484-MU
Other Names:
R2896 (G1), C57BL/6J-MtgxR2896Btlr
Major Collection:

Gene Information

Lcmt1
Name: leucine carboxyl methyltransferase 1
Synonyms: LCMT-1, Lcmt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 30949
Homologene: 41123
Gpr1
Name: G protein-coupled receptor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241070
HGNC: HGNC:4463
Homologene: 21094
Lpar6
Name: lysophosphatidic acid receptor 6
Synonyms: P2y5, P2ry5, 2610302I02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67168
Homologene: 55925
Ubn1
Name: ubinuclein 1
Synonyms: 1110029L11Rik, 2610108L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 170644
VEGA: 16
Homologene: 9656
Ttll4
Name: tubulin tyrosine ligase-like family, member 4
Synonyms: 4632407P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67534
Homologene: 8764
Pdcl
Name: phosducin-like
Synonyms: 1200011E13Rik, PhLP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67466
HGNC: HGNC:8770
Homologene: 38043
Lrrk2
Name: leucine-rich repeat kinase 2
Synonyms: 9330188B09Rik, 4921513O20Rik, cI-46, LOC381026, D630001M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 66725
Homologene: 18982
Ubr4
Name: ubiquitin protein ligase E3 component n-recognin 4
Synonyms: LOC381562, A930005E13Rik, 1810009A16Rik, p600, D930005K06Rik, Zubr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 69116
Homologene: 10804
Crebbp
Name: CREB binding protein
Synonyms: CBP, KAT3A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12914
HGNC: HGNC:2348
Homologene: 68393
Ror1
Name: receptor tyrosine kinase-like orphan receptor 1
Synonyms: 2810404D04Rik, Ntrkr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 26563
Homologene: 3675
Cachd1
Name: cache domain containing 1
Synonyms: 1190007F10Rik, Vwcd1, B430218L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 320508
Homologene: 10854
Cubn
Name: cubilin (intrinsic factor-cobalamin receptor)
Synonyms: D2Wsu88e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 65969
HGNC: HGNC:2548
Homologene: 37434
Rdh10
Name: retinol dehydrogenase 10 (all-trans)
Synonyms: D1Ertd762e, m366Asp, 3110069K09Rik, 4921506A21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98711
Homologene: 14061
Phkg1
Name: phosphorylase kinase gamma 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18682
HGNC: HGNC:8930
Homologene: 68508
Prss1
Name: protease, serine 1 (trypsin 1)
Synonyms: Trygn16, Try-1, PRSS1, Try1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 114228
Homologene: 88408
Bpifb4
Name: BPI fold containing family B, member 4
Synonyms: LOC381399, Gm1006
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 381399
Homologene: 66971
Esp6
Name: exocrine gland secreted peptide 6
Synonyms: Esp5, Gm5494
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 433107
VEGA: 17
Kif21b
Name: kinesin family member 21B
Synonyms: 2610511N21Rik, N-5 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16565
Homologene: 56868
Gss
Name: glutathione synthetase
Synonyms: GS-A/GS-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14854
HGNC: HGNC:4624
Homologene: 148
Fam83d
Name: family with sequence similarity 83, member D
Synonyms: 2310007D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71878
Homologene: 23715
Skint8
Name: selection and upkeep of intraepithelial T cells 8
Synonyms: OTTMUSG00000009475
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 639774
Homologene: 106613
Spata18
Name: spermatogenesis associated 18
Synonyms: 1700067I02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 73472
Homologene: 17057
Stard6
Name: StAR-related lipid transfer (START) domain containing 6
Synonyms: 4833424I06Rik, 1700011K09Rik, 4933429L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 170461
Homologene: 12578
Ttll3
Name: tubulin tyrosine ligase-like family, member 3
Synonyms: 4833441J24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 101100
Homologene: 134361
Olfr748
Name: olfactory receptor 748
Synonyms: MOR106-9P, GA_x6K02T2PMLR-6454789-6455712
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258113
Homologene: 134081
Zfp456
Name: zinc finger protein 456
Synonyms: Rslcan-13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 408065
Homologene: 110878
Abhd14b
Name: abhydrolase domain containing 14b
Synonyms: 1810013B01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 76491
Homologene: 9125
Gm2058
Name: predicted gene 2058
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100039133
Gm8374
Name: predicted gene 8374
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Crygs
Name: crystallin, gamma S
Synonyms: Opj
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12970
VEGA: 16
HGNC: HGNC:2417
Homologene: 40695
4930415L06Rik
Name: RIKEN cDNA 4930415L06 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245511
Homologene: 133109
Pcdh19
Name: protocadherin 19
Synonyms: B530002L05Rik, LOC279653
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 279653
Homologene: 18916
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 16,106,105 bp
  • T to A, chromosome 1 at 63,183,162 bp
  • T to A, chromosome 1 at 74,685,358 bp
  • T to C, chromosome 1 at 136,154,217 bp
  • A to T, chromosome 2 at 13,486,866 bp
  • A to T, chromosome 2 at 37,355,690 bp
  • T to G, chromosome 2 at 153,954,437 bp
  • A to G, chromosome 2 at 155,564,829 bp
  • G to A, chromosome 2 at 158,785,978 bp
  • T to C, chromosome 4 at 100,096,280 bp
  • T to A, chromosome 4 at 100,970,903 bp
  • A to G, chromosome 4 at 111,950,136 bp
  • A to G, chromosome 4 at 139,455,644 bp
  • T to C, chromosome 5 at 73,657,802 bp
  • C to A, chromosome 5 at 129,864,630 bp
  • G to A, chromosome 6 at 41,463,705 bp
  • G to T, chromosome 6 at 113,392,722 bp
  • T to G, chromosome 7 at 39,589,013 bp
  • A to G, chromosome 7 at 123,421,586 bp
  • C to T, chromosome 9 at 106,450,114 bp
  • T to C, chromosome 13 at 67,367,297 bp
  • T to A, chromosome 14 at 7,364,200 bp
  • T to C, chromosome 14 at 50,710,516 bp
  • A to G, chromosome 14 at 73,239,276 bp
  • T to A, chromosome 15 at 91,814,651 bp
  • A to G, chromosome 16 at 4,138,816 bp
  • A to G, chromosome 16 at 5,055,219 bp
  • C to T, chromosome 16 at 22,805,551 bp
  • T to A, chromosome 17 at 40,562,943 bp
  • G to A, chromosome 18 at 70,476,388 bp
  • A to G, chromosome X at 89,932,399 bp
  • TGTCTCCTCCACGTC to TGTC, chromosome X at 133,681,309 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2896 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040484-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.