Strain Name:
C57BL/6J-MtgxR2966Btlr/Mmmh
Stock Number:
040522-MU
Citation ID:
RRID:MMRRC_040522-MU
Other Names:
R2966 (G1), C57BL/6J-MtgxR2966Btlr
Major Collection:

Gene Information

Col2a1
Name: collagen, type II, alpha 1
Synonyms: Col2a-1, Col2a, Del1, Col2, M100856, Rgsc856, Lpk, M100413, Rgsc413
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 12824
HGNC: HGNC:2200
Homologene: 55607
Rprd2
Name: regulation of nuclear pre-mRNA domain containing 2
Synonyms: 6720469I21Rik, 2810036A19Rik, 4930535B03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 75137
Homologene: 19997
Add1
Name: adducin 1 (alpha)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11518
HGNC: HGNC:243
Homologene: 22758
Usp48
Name: ubiquitin specific peptidase 48
Synonyms: D330022K21Rik, 2810449C13Rik, Usp31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 170707
Homologene: 12988
Bnc2
Name: basonuclin 2
Synonyms: 5031434M05Rik, 8430420F16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242509
Homologene: 18243
2610021A01Rik
Name: RIKEN cDNA 2610021A01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 668572
Zcchc8
Name: zinc finger, CCHC domain containing 8
Synonyms: 5730565F05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 70650
Homologene: 32349
Nav2
Name: neuron navigator 2
Synonyms: POMFIL2, Unc53H2, HELAD1, RAINB2, 5330421F07Rik, Rainb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 78286
Homologene: 52330
Rab21
Name: RAB21, member RAS oncogene family
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216344
VEGA: 10
Homologene: 8991
Fgd6
Name: FYVE, RhoGEF and PH domain containing 6
Synonyms: ZFYVE24, Etohd4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 13998
Homologene: 14209
Ap1s1
Name: adaptor protein complex AP-1, sigma 1
Synonyms: AP19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11769
HGNC: HGNC:559
Homologene: 20342
Rasal1
Name: RAS protein activator like 1 (GAP1 like)
Synonyms: MRASAL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19415
HGNC: HGNC:9873
Homologene: 3423
Chrna2
Name: cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal)
Synonyms: Acra-2, Acra2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 110902
HGNC: HGNC:1956
Homologene: 20193
Nsd2
Name: nuclear receptor binding SET domain protein 2
Synonyms: 5830445G22Rik, Whsc1l, 9430010A17Rik, D030027O06Rik, C130020C13Rik, D930023B08Rik, Whsc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 107823
Homologene: 26175
Chsy1
Name: chondroitin sulfate synthase 1
Synonyms: skt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269941
Homologene: 8950
Fstl3
Name: follistatin-like 3
Synonyms: Flrg, E030038F23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 83554
HGNC: HGNC:3973
Homologene: 4280
Sympk
Name: symplekin
Synonyms: 4632415H16Rik, 1500016F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68188
Homologene: 37969
Prss35
Name: protease, serine 35
Synonyms: 6030424L22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244954
Homologene: 17734
Pclo
Name: piccolo (presynaptic cytomatrix protein)
Synonyms: Acz, Pico
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 26875
Homologene: 69111
Gabra5
Name: gamma-aminobutyric acid (GABA) A receptor, subunit alpha 5
Synonyms: A230018I05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 110886
HGNC: HGNC:4079
Homologene: 20219
Rbm15b
Name: RNA binding motif protein 15B
Synonyms: 1810017N16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 109095
Homologene: 8325
Mgam
Name: maltase-glucoamylase
Synonyms: 6030407P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232714
HGNC: HGNC:7043
Homologene: 130099
Sis
Name: sucrase isomaltase (alpha-glucosidase)
Synonyms: Si-s, sucrase-isomaltase, 2010204N08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 69983
Homologene: 37424
Slc4a5
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 5
Synonyms: C330016K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232156
Homologene: 31125
Ltf
Name: lactotransferrin
Synonyms: Lf, lactoferrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17002
HGNC: HGNC:6720
Homologene: 1754
Myh1
Name: myosin, heavy polypeptide 1, skeletal muscle, adult
Synonyms: MyHC-IId/x, Myhs-f2, Myhs-f, Myhsf2, A530084A17Rik, MYHC-IIX, myosin heavy chain 2X, IId, IId/x
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17879
HGNC: HGNC:7567
Homologene: 133718
Lrriq1
Name: leucine-rich repeats and IQ motif containing 1
Synonyms: LOC380658, 4930503E15Rik, Gm1557
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 74978
Homologene: 46007
Cfap300
Name: cilia and flagella associated protein 300
Synonyms: 9230110C19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 234912
VEGA: 9
Homologene: 13163
Adamts1
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 1
Synonyms: ADAM-TS1, METH1, METH-1, ADAMTS-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 11504
HGNC: HGNC:217
Homologene: 21381
Atp11a
Name: ATPase, class VI, type 11A
Synonyms: Ih, 4930558F19Rik, 9130422H11Rik, LOC100045280
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 50770
Homologene: 75050
Fbxw21
Name: F-box and WD-40 domain protein 21
Synonyms: E330009P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320082
Homologene: 110776
Noa1
Name: nitric oxide associated 1
Synonyms: mAtNOS1, 2610024G14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 56412
Homologene: 10518
Vmn1r39
Name: vomeronasal 1 receptor 39
Synonyms: Gm5993
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 546903
Homologene: 110800
Has2
Name: hyaluronan synthase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 15117
HGNC: HGNC:4819
Homologene: 3892
Atp8a1
Name: ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
Synonyms: Atp3a2, B230107D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11980
Homologene: 48402
Asprv1
Name: aspartic peptidase, retroviral-like 1
Synonyms: TPA-induced aspartic proteinase-like, Taps, SASPase, SASP, 2300003P22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67855
Homologene: 23582
Grip2
Name: glutamate receptor interacting protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243547
Homologene: 16327
Pnpla2
Name: patatin-like phospholipase domain containing 2
Synonyms: 1110001C14Rik, 0610039C21Rik, Atgl, desnutrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 66853
Homologene: 10687
Cep250
Name: centrosomal protein 250
Synonyms: Inmp, B230210E21Rik, Cep2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16328
HGNC: HGNC:1859
Homologene: 38286
Gpr45
Name: G protein-coupled receptor 45
Synonyms: PSP24alpha, 9230112G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 93690
HGNC: HGNC:4503
Homologene: 5228
Recql
Name: RecQ protein-like
Synonyms: RecQ1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 19691
HGNC: HGNC:9948
Homologene: 56279
Gm6871
Name: predicted gene 6871
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 102642386
Dyrk2
Name: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2
Synonyms: 1810038L18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 69181
VEGA: 10
HGNC: HGNC:3093
Homologene: 48437
Ddx43
Name: DEAD box helicase 43
Synonyms: ENSMUSG00000070291, OTTMUSG00000019690, DEAD (Asp-Glu-Ala-Asp) box polypeptide 43
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 100048658
Homologene: 56814
Gatd1
Name: glutamine amidotransferase like class 1 domain containing 1
Synonyms: D230016J19Rik, Pddc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 213350
Homologene: 35234
Bahd1
Name: bromo adjacent homology domain containing 1
Synonyms: LOC228536
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228536
Homologene: 8976
Gm10542
Name: predicted gene 10542
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Rpl31-ps17
Name: ribosomal protein L31, pseudogene 17
Synonyms: Gm16382
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 673582
Pth
Name: parathyroid hormone
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19226
HGNC: HGNC:9606
Homologene: 266
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 43,032,508 bp
  • C to T, chromosome 2 at 118,916,406 bp
  • A to G, chromosome 2 at 155,994,878 bp
  • T to A, chromosome 3 at 72,889,010 bp
  • A to T, chromosome 3 at 95,766,433 bp
  • G to A, chromosome 4 at 84,293,517 bp
  • G to A, chromosome 4 at 137,613,762 bp
  • T to C, chromosome 5 at 14,681,150 bp
  • G to C, chromosome 5 at 33,846,122 bp
  • C to A, chromosome 5 at 34,630,714 bp
  • T to C, chromosome 5 at 67,647,706 bp
  • T to C, chromosome 5 at 77,306,344 bp
  • T to A, chromosome 5 at 120,671,620 bp
  • A to G, chromosome 5 at 123,720,867 bp
  • T to C, chromosome 5 at 137,037,503 bp
  • T to C, chromosome 6 at 40,768,220 bp
  • A to C, chromosome 6 at 66,804,731 bp
  • C to T, chromosome 6 at 83,296,669 bp
  • T to A, chromosome 6 at 86,628,366 bp
  • T to C, chromosome 6 at 91,777,517 bp
  • A to G, chromosome 6 at 142,363,587 bp
  • T to C, chromosome 7 at 19,030,544 bp
  • T to G, chromosome 7 at 41,573,440 bp
  • C to T, chromosome 7 at 41,626,405 bp
  • C to T, chromosome 7 at 49,557,032 bp
  • T to C, chromosome 7 at 57,408,641 bp
  • G to A, chromosome 7 at 66,172,164 bp
  • T to C, chromosome 7 at 113,385,929 bp
  • T to A, chromosome 7 at 141,409,167 bp
  • C to T, chromosome 7 at 141,458,478 bp
  • A to T, chromosome 8 at 12,847,853 bp
  • C to T, chromosome 9 at 8,027,174 bp
  • C to A, chromosome 9 at 78,406,379 bp
  • A to G, chromosome 9 at 86,755,582 bp
  • A to G, chromosome 9 at 106,885,592 bp
  • A to G, chromosome 9 at 109,145,510 bp
  • G to T, chromosome 9 at 111,028,472 bp
  • T to C, chromosome 10 at 79,781,223 bp
  • T to A, chromosome 10 at 94,044,194 bp
  • A to G, chromosome 10 at 103,214,900 bp
  • T to C, chromosome 10 at 115,294,909 bp
  • T to A, chromosome 10 at 118,860,337 bp
  • A to G, chromosome 11 at 67,214,584 bp
  • C to T, chromosome 12 at 54,701,612 bp
  • T to C, chromosome 14 at 66,149,368 bp
  • A to G, chromosome 15 at 56,682,137 bp
  • A to G, chromosome 15 at 97,976,095 bp
  • A to G, chromosome 16 at 85,796,774 bp
  • A to T, chromosome 18 at 44,204,695 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2966 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040522-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.