Strain Name:
C57BL/6J-MtgxR3013Btlr/Mmmh
Stock Number:
040534-MU
Citation ID:
RRID:MMRRC_040534-MU
Other Names:
R3013 (G1), C57BL/6J-MtgxR3013Btlr
Major Collection:

Gene Information

Alg10b
Name: asparagine-linked glycosylation 10B (alpha-1,2-glucosyltransferase)
Synonyms: LOC380959, Deaf1, nse5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 380959
VEGA: 15
Homologene: 6030
Gtf2i
Name: general transcription factor II I
Synonyms: 6030441I21Rik, TFII-I, BAP-135
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 14886
HGNC: HGNC:4659
Homologene: 7748
Plcb4
Name: phospholipase C, beta 4
Synonyms: A930039J07Rik, C230058B11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 18798
HGNC: HGNC:9059
Homologene: 8471
Ash2l
Name: ASH2 like histone lysine methyltransferase complex subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 23808
HGNC: HGNC:744
Homologene: 3436
Rbfox2
Name: RNA binding protein, fox-1 homolog (C. elegans) 2
Synonyms: Rbm9, Fbm2, Fxh, 2810460A15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 93686
VEGA: 15
HGNC: HGNC:9906
Homologene: 49375
Arid2
Name: AT rich interactive domain 2 (ARID, RFX-like)
Synonyms: zipzap/p200, 4432409D24Rik, 1700124K17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 77044
Homologene: 14601
Rsf1
Name: remodeling and spacing factor 1
Synonyms: XAP8, p325, 4832420A03Rik, C030033M12Rik, Hbxap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233532
Homologene: 41142
Hip1
Name: huntingtin interacting protein 1
Synonyms: A930014B11Rik, HIP-1, 2610109B09Rik, E130315I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 215114
HGNC: HGNC:4913
Homologene: 68463
Scp2
Name: sterol carrier protein 2, liver
Synonyms: nonspecific lipid transfer protein, ns-LTP, SCP-2, SCPx, NSL-TP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 20280
Homologene: 37717
Lrpprc
Name: leucine-rich PPR-motif containing
Synonyms: Lrp130, 3110001K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 72416
Homologene: 32695
Smad4
Name: SMAD family member 4
Synonyms: D18Wsu70e, DPC4, Madh4, Dpc4, Smad 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 17128
HGNC: HGNC:6770
Homologene: 31310
Hspbp1
Name: HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1
Synonyms: 1500019G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 66245
Homologene: 40827
Afg3l1
Name: AFG3-like AAA ATPase 1
Synonyms: 3110061K15Rik, 1700047G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 114896
HGNC: HGNC:314
Homologene: 134198
Eml5
Name: echinoderm microtubule associated protein like 5
Synonyms: C130068M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 319670
VEGA: 12
Homologene: 26807
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Ryr3
Name: ryanodine receptor 3
Synonyms: calcium release channel isoform 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20192
Homologene: 68151
Bpifb5
Name: BPI fold containing family B, member 5
Synonyms: BC018465
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228802
Homologene: 64814
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 380698
Homologene: 70869
Serpina3j
Name: serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3J
Synonyms: Gm4931, alpha-1 antiproteinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238395
HGNC: HGNC:16
Homologene: 120222
Ccdc39
Name: coiled-coil domain containing 39
Synonyms: 4921507O14Rik, D3Ertd789e, b2b1735Clo, b2b1304Clo, b2b2025.1Clo, prh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 51938
Homologene: 12149
Zfp235
Name: zinc finger protein 235
Synonyms: 0610030O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 56525
Homologene: 122146
Pcnx2
Name: pecanex homolog 2
Synonyms: E330039K12Rik, Pcnxl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 270109
HGNC: HGNC:8736
Homologene: 8987
Dnah7b
Name: dynein, axonemal, heavy chain 7B
Synonyms: Dnahc7b, LOC227058
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 227058
Homologene: 41287
Ces1e
Name: carboxylesterase 1E
Synonyms: egasyn, Es-22, Eg, Es22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 13897
HGNC: HGNC:1863
Homologene: 115660
Ccdc57
Name: coiled-coil domain containing 57
Synonyms: 4933434G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 71276
Homologene: 52351
AC168120.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
Xirp1
Name: xin actin-binding repeat containing 1
Synonyms: mXin alpha, Cmya1, Xin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 22437
VEGA: 9
Homologene: 7998
Gm13119
Name: predicted gene 13119
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 433779
Homologene: 83161
Prr5l
Name: proline rich 5 like
Synonyms: 2600010E01Rik, 4833411O04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 72446
Homologene: 11753
Mkx
Name: mohawk homeobox
Synonyms: 9430023B20Rik, Irxl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 210719
Homologene: 72239
Cyp4f14
Name: cytochrome P450, family 4, subfamily f, polypeptide 14
Synonyms: leukotriene B4 omega hydroxylase, 1300014O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 64385
VEGA: 17
Homologene: 81872
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 46,188,687 bp
  • T to C, chromosome 2 at 76,734,681 bp
  • A to G, chromosome 2 at 101,734,705 bp
  • A to T, chromosome 2 at 112,640,281 bp
  • A to G, chromosome 2 at 135,947,169 bp
  • T to C, chromosome 2 at 154,228,855 bp
  • C to T, chromosome 3 at 33,814,668 bp
  • A to G, chromosome 3 at 75,450,847 bp
  • T to A, chromosome 4 at 108,071,357 bp
  • T to A, chromosome 4 at 144,362,455 bp
  • T to C, chromosome 5 at 134,295,504 bp
  • T to A, chromosome 5 at 135,435,039 bp
  • G to T, chromosome 7 at 4,663,484 bp
  • A to G, chromosome 7 at 24,140,732 bp
  • ATGGCG to ATGGCGACGGTGGCG, chromosome 7 at 97,579,904 bp
  • T to C, chromosome 8 at 25,839,764 bp
  • G to A, chromosome 8 at 93,203,287 bp
  • C to T, chromosome 8 at 123,484,677 bp
  • C to A, chromosome 8 at 125,887,770 bp
  • T to C, chromosome 9 at 120,019,785 bp
  • A to T, chromosome 11 at 59,060,918 bp
  • A to G, chromosome 11 at 120,861,199 bp
  • T to C, chromosome 12 at 98,880,808 bp
  • A to G, chromosome 12 at 104,319,707 bp
  • T to C, chromosome 15 at 77,132,920 bp
  • T to A, chromosome 15 at 90,227,556 bp
  • T to C, chromosome 15 at 96,361,936 bp
  • T to C, chromosome 17 at 32,909,165 bp
  • T to C, chromosome 17 at 84,767,069 bp
  • A to T, chromosome 18 at 6,936,929 bp
  • T to C, chromosome 18 at 73,648,904 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3013 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040534-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.