Strain Name:
C57BL/6J-MtgxR3016Btlr/Mmmh
Stock Number:
040537-MU
Citation ID:
RRID:MMRRC_040537-MU
Other Names:
R3016 (G1), C57BL/6J-MtgxR3016Btlr
Major Collection:

Gene Information

Meis1
Name: Meis homeobox 1
Synonyms: C530044H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17268
HGNC: HGNC:7000
Homologene: 86803
Fancd2
Name: Fanconi anemia, complementation group D2
Synonyms: 2410150O07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 211651
HGNC: HGNC:3585
Homologene: 13212
Traip
Name: TRAF-interacting protein
Synonyms: Trip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 22036
Homologene: 31343
Tspan14
Name: tetraspanin 14
Synonyms: D14Ertd226e, Tm4sf14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 52588
VEGA: 14
Homologene: 23717
Alkbh8
Name: alkB homolog 8, tRNA methyltransferase
Synonyms: 8030431D03Rik, 4930562C03Rik, 9430088N01Rik, Abh8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67667
Homologene: 44488
Foxo3
Name: forkhead box O3
Synonyms: FKHRL1, Fkhr2, 2010203A17Rik, 1110048B16Rik, Foxo3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 56484
HGNC: HGNC:3821
Homologene: 31039
Pole2
Name: polymerase (DNA directed), epsilon 2 (p59 subunit)
Synonyms: DNA polymerase epsilon small subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18974
VEGA: 12
HGNC: HGNC:9178
Homologene: 2015
Rapgef1
Name: Rap guanine nucleotide exchange factor (GEF) 1
Synonyms: C3G, 4932418O06Rik, Grf2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 107746
HGNC: HGNC:4568
Homologene: 50501
Xpo5
Name: exportin 5
Synonyms: 2410004H11Rik, 2700038C24Rik, Exp5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72322
VEGA: 17
Homologene: 69316
Gna14
Name: guanine nucleotide binding protein, alpha 14
Synonyms: G alpha 14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 14675
VEGA: 19
HGNC: HGNC:4382
Homologene: 68386
Sbsn
Name: suprabasin
Synonyms: 1110005D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 282619
Homologene: 17900
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: D86 mRNA, PKHDL1, fibrocystin L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 192190
Homologene: 16332
Olfr169
Name: olfactory receptor 169
Synonyms: GA_x54KRFPKG5P-16014972-16014031, MOR273-3P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258158
Homologene: 128058
Fam38b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Hecw2
Name: HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Synonyms: D030049F17Rik, Nedl2, A730039N16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329152
Homologene: 66192
Tmed6
Name: transmembrane p24 trafficking protein 6
Synonyms: 1810018I24Rik, 1810015P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66269
Homologene: 11938
4732465J04Rik
Name: RIKEN cDNA 4732465J04 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 414105
Fam38a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Adam22
Name: a disintegrin and metallopeptidase domain 22
Synonyms: MDC2, 2900022I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11496
HGNC: HGNC:201
Homologene: 37898
Ptprb
Name: protein tyrosine phosphatase, receptor type, B
Synonyms: 3230402H02Rik, VE-PTP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19263
VEGA: 10
HGNC: HGNC:9665
Homologene: 2125
Cep250
Name: centrosomal protein 250
Synonyms: Inmp, B230210E21Rik, Cep2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16328
HGNC: HGNC:1859
Homologene: 38286
Padi3
Name: peptidyl arginine deiminase, type III
Synonyms: PAD type III, Pad3, Pdi3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18601
Homologene: 7882
Kcnd3
Name: potassium voltage-gated channel, Shal-related family, member 3
Synonyms: potassium channel Kv4.3M, potassium channel Kv4.3L, Kv4.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 56543
HGNC: HGNC:6239
Homologene: 21036
Asgr2
Name: asialoglycoprotein receptor 2
Synonyms: Asgr-2, Asgr, ASGPR2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11890
HGNC: HGNC:743
Homologene: 912
Lag3
Name: lymphocyte-activation gene 3
Synonyms: CD223, Ly66, LAG-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16768
HGNC: HGNC:6476
Homologene: 1719
Gm10088
Name: predicted gene 10088
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 100504250
2810408A11Rik
Name: RIKEN cDNA 2810408A11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70419
Homologene: 49883
Olfr968
Name: olfactory receptor 968
Synonyms: GA_x6K02T2PVTD-33470347-33469403, MOR171-15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258605
VEGA: 9
Homologene: 121532
Idi2
Name: isopentenyl-diphosphate delta isomerase 2
Synonyms: 4833405L16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 320581
Homologene: 136408
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 53,830,680 bp
  • G to A, chromosome 2 at 29,707,393 bp
  • A to G, chromosome 2 at 155,991,288 bp
  • C to T, chromosome 3 at 105,658,766 bp
  • G to T, chromosome 4 at 140,786,587 bp
  • A to T, chromosome 5 at 8,134,896 bp
  • T to C, chromosome 6 at 113,536,726 bp
  • A to G, chromosome 6 at 124,908,466 bp
  • T to C, chromosome 7 at 30,754,997 bp
  • A to G, chromosome 8 at 107,065,437 bp
  • A to G, chromosome 8 at 122,506,027 bp
  • G to T, chromosome 9 at 3,369,658 bp
  • A to T, chromosome 9 at 39,772,683 bp
  • T to C, chromosome 9 at 107,963,423 bp
  • G to T, chromosome 10 at 42,197,356 bp
  • A to G, chromosome 10 at 95,793,832 bp
  • T to C, chromosome 10 at 116,357,295 bp
  • A to T, chromosome 11 at 19,011,238 bp
  • T to C, chromosome 11 at 69,899,222 bp
  • A to T, chromosome 11 at 70,105,409 bp
  • A to G, chromosome 12 at 69,222,062 bp
  • T to C, chromosome 13 at 8,959,430 bp
  • T to C, chromosome 14 at 40,918,621 bp
  • G to A, chromosome 15 at 44,545,370 bp
  • T to G, chromosome 16 at 19,028,550 bp
  • A to G, chromosome 16 at 19,566,391 bp
  • A to G, chromosome 17 at 46,220,831 bp
  • G to A, chromosome 18 at 63,042,832 bp
  • A to T, chromosome 19 at 16,603,382 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3016 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040537-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.