Strain Name:
C57BL/6J-MtgxR3029Btlr/Mmmh
Stock Number:
040545-MU
Citation ID:
RRID:MMRRC_040545-MU
Other Names:
R3029 (G1), C57BL/6J-MtgxR3029Btlr
Major Collection:

Gene Information

Cdk6
Name: cyclin-dependent kinase 6
Synonyms: Crk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12571
HGNC: HGNC:1777
Homologene: 963
Sparcl1
Name: SPARC-like 1
Synonyms: mast9, Ecm2, hevin, Sc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13602
Homologene: 3438
Cryab
Name: crystallin, alpha B
Synonyms: Crya2, alpha B-crystallin, Crya-2, HspB5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12955
VEGA: 9
HGNC: HGNC:2389
Homologene: 68209
E2f5
Name: E2F transcription factor 5
Synonyms: E2F-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 13559
HGNC: HGNC:3119
Homologene: 1472
Gad1
Name: glutamate decarboxylase 1
Synonyms: Gad-1, Z49976, GAD44, EP10, GAD67, GAD25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14415
HGNC: HGNC:4092
Homologene: 635
Eif5b
Name: eukaryotic translation initiation factor 5B
Synonyms: A030003E17Rik, IF2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226982
Homologene: 134613
Rbm48
Name: RNA binding motif protein 48
Synonyms: C030048B08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269623
Homologene: 12944
Fat2
Name: FAT atypical cadherin 2
Synonyms: Fath2, EMI2, LOC245827, mKIAA0811
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Pkdrej
Name: polycystin (PKD) family receptor for egg jelly
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18766
VEGA: 15
HGNC: HGNC:9015
Homologene: 4427
Nlrp1a
Name: NLR family, pyrin domain containing 1A
Synonyms: Nalp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 195046
Homologene: 133820
Itgad
Name: integrin, alpha D
Synonyms: Cd11d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381924
HGNC: HGNC:6146
Homologene: 56919
Nsun4
Name: NOL1/NOP2/Sun domain family, member 4
Synonyms: 2810405F18Rik, 2310010O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 72181
Homologene: 12455
Gm281
Name: predicted gene 281
Synonyms: LOC238939
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 238939
Homologene: 141164
Rxfp2
Name: relaxin/insulin-like family peptide receptor 2
Synonyms: Great, LGR8, Gpr106
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 140498
Homologene: 15402
Kcnh1
Name: potassium voltage-gated channel, subfamily H (eag-related), member 1
Synonyms: ether a go-go, Kv10.1, Eag1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16510
HGNC: HGNC:6250
Homologene: 68242
Proz
Name: protein Z, vitamin K-dependent plasma glycoprotein
Synonyms: 1300015B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66901
HGNC: HGNC:9460
Homologene: 2890
Mrc2
Name: mannose receptor, C type 2
Synonyms: uPARAP, Endo180, novel lectin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17534
Homologene: 4408
Cdh15
Name: cadherin 15
Synonyms: Mcad, Cdh14, M cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12555
HGNC: HGNC:1754
Homologene: 3622
Vmn2r14
Name: vomeronasal 2, receptor 14
Synonyms: EG231591
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231591
Homologene: 129606
Atp8a2
Name: ATPase, aminophospholipid transporter-like, class I, type 8A, member 2
Synonyms: wl, agil, Ib
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50769
Homologene: 4443
Gm4825
Name: predicted pseudogene 4825
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223745
Eya4
Name: EYA transcriptional coactivator and phosphatase 4
Synonyms: B130023L16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 14051
VEGA: 10
HGNC: HGNC:3522
Homologene: 3025
A630001G21Rik
Name: RIKEN cDNA A630001G21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 319997
Homologene: 79992
H2-M11
Name: histocompatibility 2, M region locus 11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224754
Homologene: 52241
Rgs20
Name: regulator of G-protein signaling 20
Synonyms: Rgsz1, 2900073E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 58175
Homologene: 2745
Zfp61
Name: zinc finger protein 61
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22719
Homologene: 74923
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 5,070,053 bp
  • T to C, chromosome 1 at 38,018,011 bp
  • A to G, chromosome 1 at 85,718,245 bp
  • C to T, chromosome 1 at 192,506,060 bp
  • G to A, chromosome 2 at 70,594,690 bp
  • A to G, chromosome 3 at 14,603,665 bp
  • A to G, chromosome 4 at 116,052,725 bp
  • G to A, chromosome 5 at 3,390,817 bp
  • A to T, chromosome 5 at 3,596,043 bp
  • T to C, chromosome 5 at 104,093,226 bp
  • T to A, chromosome 5 at 109,215,910 bp
  • A to C, chromosome 5 at 150,043,130 bp
  • A to T, chromosome 7 at 24,293,335 bp
  • T to A, chromosome 7 at 128,178,371 bp
  • A to T, chromosome 8 at 13,061,042 bp
  • G to A, chromosome 8 at 122,862,024 bp
  • T to A, chromosome 9 at 50,756,338 bp
  • T to C, chromosome 10 at 23,123,878 bp
  • T to C, chromosome 11 at 55,284,709 bp
  • T to A, chromosome 11 at 71,123,630 bp
  • G to A, chromosome 11 at 105,348,431 bp
  • G to A, chromosome 14 at 13,831,543 bp
  • CGT to CGTGT, chromosome 14 at 59,691,465 bp
  • A to G, chromosome 15 at 85,511,194 bp
  • C to T, chromosome 15 at 85,817,004 bp
  • C to T, chromosome 17 at 36,548,150 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3029 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040545-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.