Strain Name:
C57BL/6J-MtgxR3033Btlr/Mmmh
Stock Number:
040549-MU
Citation ID:
RRID:MMRRC_040549-MU
Other Names:
R3033 (G1), C57BL/6J-MtgxR3033Btlr
Major Collection:

Gene Information

Nr2f2
Name: nuclear receptor subfamily 2, group F, member 2
Synonyms: COUP-TFII, Aporp1, 9430015G03Rik, EAR3, ARP-1, COUP-TF2, Tcfcoup2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11819
HGNC: HGNC:7976
Homologene: 7628
Dst
Name: dystonin
Synonyms: 2310001O04Rik, athetoid, nmf339, Macf2, ah, nmf203, A830042E19Rik, BPAG1, Bpag, BPAG1-n, bullous pemphigoid antigen 1, bullous pemphigoid antigen 1, Bpag1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: nRapGEP, 5830453M24Rik, Pdzgef1, RA-GEF-1, CNRasGEF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 76089
Homologene: 35477
Eif4g3
Name: eukaryotic translation initiation factor 4 gamma, 3
Synonyms: G1-419-52, repro8, 1500002J22Rik, eIF4GII, 4930523M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230861
HGNC: HGNC:3298
Homologene: 2789
Mcm3
Name: minichromosome maintenance complex component 3
Synonyms: P1, p1.m, Mcmd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17215
HGNC: HGNC:6945
Homologene: 1791
Kmt2a
Name: lysine (K)-specific methyltransferase 2A
Synonyms: Cxxc7, Mll, ALL-1, trithorax Drosophila, HTRX1, All1, Mll1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214162
HGNC: HGNC:7132
Homologene: 4338
Heatr5a
Name: HEAT repeat containing 5A
Synonyms: D930036F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 320487
VEGA: 12
Homologene: 19635
Dennd4c
Name: DENN/MADD domain containing 4C
Synonyms: 1700065A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329877
Homologene: 23057
Myo9b
Name: myosin IXb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 17925
HGNC: HGNC:7609
Homologene: 3058
Tomm70a
Name: translocase of outer mitochondrial membrane 70A
Synonyms: 2610044B22Rik, Tomm70a, D16Wsu109e, D16Ium22, Tom70, D16Ium22e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 28185
VEGA: 16
Homologene: 40112
Lpcat1
Name: lysophosphatidylcholine acyltransferase 1
Synonyms: LPCAT, Aytl2, rd11, 2900035H07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 210992
Homologene: 9871
Lypd8
Name: LY6/PLAUR domain containing 8
Synonyms: 2210415F13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70163
Homologene: 52815
Aqp4
Name: aquaporin 4
Synonyms: aquaporin-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 11829
VEGA: 18
HGNC: HGNC:637
Homologene: 37507
Ercc5
Name: excision repair cross-complementing rodent repair deficiency, complementation group 5
Synonyms: Xpg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22592
HGNC: HGNC:3437
Homologene: 133551
Rap1gap2
Name: RAP1 GTPase activating protein 2
Synonyms: Garnl4, LOC380710
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380711
Homologene: 56695
Dnah6
Name: dynein, axonemal, heavy chain 6
Synonyms: Dnahc6, A730004I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330355
HGNC: HGNC:2951
Homologene: 15221
Astn2
Name: astrotactin 2
Synonyms: 1d8, Astnl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56079
Homologene: 77850
Naip1
Name: NLR family, apoptosis inhibitory protein 1
Synonyms: Naip, D13Lsd1, Birc1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17940
VEGA: 13
HGNC: HGNC:7634
Homologene: 113589
Tpte
Name: transmembrane phosphatase with tensin homology
Synonyms: Vsp, Pten2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234129
Homologene: 50411
Tlr1
Name: toll-like receptor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21897
Homologene: 20694
Smg9
Name: SMG9 nonsense mediated mRNA decay factor
Synonyms: 1500002O20Rik, smg-9 homolog, nonsense mediated mRNA decay factor (C. elegans), N28092
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71997
Homologene: 10426
Ift88
Name: intraflagellar transport 88
Synonyms: fxo, Ttc10, polaris, Tg737, IFT88, TgN737Rpw, Oak Ridge polycystic kidneys, Tg737Rpw, orpk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21821
Homologene: 4761
Selenbp1
Name: selenium binding protein 1
Synonyms: Lpsb, Lp56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20341
Homologene: 2930
Adam3
Name: a disintegrin and metallopeptidase domain 3 (cyritestin)
Synonyms: tMDC, ADAM3, Taz83, Taz83, Cyrn1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11497
HGNC: HGNC:209
Homologene: 69052
Zfp58
Name: zinc finger protein 58
Synonyms: A530094I17Rik, Mfg1, Mfg-1, Zfp817
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238693
Bahd1
Name: bromo adjacent homology domain containing 1
Synonyms: LOC228536
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228536
Homologene: 8976
Neurl2
Name: neuralized E3 ubiquitin protein ligase 2
Synonyms: Ozz, Ozz-E3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 415115
Homologene: 15435
Gm5414
Name: predicted gene 5414
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 406223
VEGA: 15
Rab36
Name: RAB36, member RAS oncogene family
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 76877
HGNC: HGNC:9775
Homologene: 3610
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 20,808,768 bp
  • T to C, chromosome 1 at 34,152,285 bp
  • A to G, chromosome 1 at 44,180,574 bp
  • C to T, chromosome 2 at 118,916,406 bp
  • T to C, chromosome 2 at 164,833,055 bp
  • A to G, chromosome 3 at 79,074,306 bp
  • T to C, chromosome 3 at 94,938,040 bp
  • T to C, chromosome 4 at 65,644,706 bp
  • AGGAGCTCCTGGAGC to AGGAGC, chromosome 4 at 86,825,320 bp
  • A to T, chromosome 4 at 138,103,410 bp
  • T to C, chromosome 5 at 64,925,569 bp
  • T to C, chromosome 6 at 73,173,350 bp
  • A to G, chromosome 7 at 24,416,524 bp
  • A to G, chromosome 7 at 70,358,062 bp
  • T to A, chromosome 8 at 22,320,872 bp
  • T to C, chromosome 8 at 24,694,211 bp
  • G to A, chromosome 8 at 71,334,337 bp
  • A to G, chromosome 9 at 44,821,863 bp
  • G to A, chromosome 10 at 75,044,496 bp
  • T to C, chromosome 11 at 58,384,627 bp
  • C to A, chromosome 11 at 74,407,322 bp
  • A to T, chromosome 12 at 51,951,038 bp
  • T to C, chromosome 13 at 67,491,622 bp
  • C to T, chromosome 13 at 73,503,105 bp
  • T to C, chromosome 13 at 100,432,458 bp
  • G to A, chromosome 14 at 57,478,044 bp
  • T to C, chromosome 15 at 101,624,609 bp
  • G to A, chromosome 16 at 57,122,025 bp
  • T to A, chromosome 18 at 15,393,560 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3033 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040549-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.