Strain Name:
C57BL/6J-MtgxR3035Btlr/Mmmh
Stock Number:
040551-MU
Citation ID:
RRID:MMRRC_040551-MU
Other Names:
R3035 (G1), C57BL/6J-MtgxR3035Btlr
Major Collection:

Gene Information

Gsc2
Name: goosecoid homebox 2
Synonyms: 4930568H22Rik, Gscl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 195333
HGNC: HGNC:4613
Homologene: 3884
Slc12a5
Name: solute carrier family 12, member 5
Synonyms: KCC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 57138
Homologene: 10665
Topors
Name: topoisomerase I binding, arginine/serine-rich
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 106021
Homologene: 4237
Hcn4
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 330953
VEGA: 9
Homologene: 3997
Dock9
Name: dedicator of cytokinesis 9
Synonyms: Zizimin1, B230309H04Rik, D14Wsu89e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 105445
Homologene: 41026
Kif11
Name: kinesin family member 11
Synonyms: Knsl1, Kifl1, Eg5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 16551
VEGA: 19
HGNC: HGNC:6388
Homologene: 3322
Ahctf1
Name: AT hook containing transcription factor 1
Synonyms: 6230412P20Rik, Elys
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 226747
Homologene: 9142
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, 2810449H11Rik, tbl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Cd55
Name: CD55 molecule, decay accelerating factor for complement
Synonyms: Daf1, Cromer blood group, complement-glycosylphosphatidylinositol, GPI-DAF, Daf-GPI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 13136
HGNC: HGNC:2665
Homologene: 479
Rap1gap2
Name: RAP1 GTPase activating protein 2
Synonyms: Garnl4, LOC380710
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 380711
Homologene: 56695
Chia1
Name: chitinase, acidic 1
Synonyms: YNL, Chia, AMCase, 2200003E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 81600
Homologene: 75168
Mgam
Name: maltase-glucoamylase
Synonyms: 6030407P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232714
HGNC: HGNC:7043
Homologene: 130099
Dennd5a
Name: DENN/MADD domain containing 5A
Synonyms: ORF37, Rab6ip1, 1500012B19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 19347
Homologene: 14584
C1galt1
Name: core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1
Synonyms: T-synthase, 2210410E06Rik, core 1 beta3-Gal-T
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 94192
Homologene: 10599
Mbl1
Name: mannose-binding lectin (protein A) 1
Synonyms: MBL-A, MBP-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 17194
VEGA: 14
HGNC: HGNC:6921
Homologene: 55449
Serpinb9b
Name: serine (or cysteine) peptidase inhibitor, clade B, member 9b
Synonyms: 1600019A21Rik, ovalbumin, Spi10, R86, SPI-CI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 20706
HGNC: HGNC:8955
Homologene: 137240
4932414N04Rik
Name: RIKEN cDNA 4932414N04 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 75721
Homologene: 138468
Apba2
Name: amyloid beta (A4) precursor protein-binding, family A, member 2
Synonyms: X11-like, Mint 2, X11L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 11784
HGNC: HGNC:579
Homologene: 4021
9130230L23Rik
Name: RIKEN cDNA 9130230L23 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231253
Pbxip1
Name: pre B cell leukemia transcription factor interacting protein 1
Synonyms: 4732463H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229534
Homologene: 10740
Rab36
Name: RAB36, member RAS oncogene family
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 76877
HGNC: HGNC:9775
Homologene: 3610
Vta1
Name: vesicle (multivesicular body) trafficking 1
Synonyms: 1110059P08Rik, 1110001D18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 66201
Homologene: 6473
Kdf1
Name: keratinocyte differentiation factor 1
Synonyms: 1810019J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 69073
Homologene: 17563
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 130,458,079 bp
  • T to A, chromosome 1 at 179,753,870 bp
  • T to A, chromosome 2 at 68,745,418 bp
  • C to A, chromosome 2 at 164,980,258 bp
  • T to C, chromosome 3 at 89,445,685 bp
  • A to C, chromosome 3 at 106,130,611 bp
  • C to T, chromosome 4 at 40,269,673 bp
  • A to G, chromosome 4 at 133,528,062 bp
  • T to A, chromosome 5 at 65,989,921 bp
  • A to G, chromosome 6 at 7,866,762 bp
  • A to G, chromosome 6 at 40,663,530 bp
  • T to C, chromosome 7 at 64,739,792 bp
  • A to G, chromosome 7 at 109,921,352 bp
  • T to C, chromosome 9 at 58,823,680 bp
  • C to A, chromosome 9 at 66,483,935 bp
  • G to A, chromosome 10 at 14,661,155 bp
  • G to A, chromosome 10 at 75,044,496 bp
  • C to A, chromosome 11 at 74,407,322 bp
  • T to A, chromosome 13 at 33,029,546 bp
  • C to A, chromosome 14 at 41,158,833 bp
  • A to G, chromosome 14 at 121,606,837 bp
  • A to G, chromosome 16 at 17,914,928 bp
  • T to A, chromosome 19 at 37,407,053 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3035 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040551-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.