Strain Name:
C57BL/6J-MtgxR3036Btlr/Mmmh
Stock Number:
040552-MU
Citation ID:
RRID:MMRRC_040552-MU
Other Names:
R3036 (G1), C57BL/6J-MtgxR3036Btlr
Major Collection:

Strain Information

Mdn1
Name: midasin AAA ATPase 1
Synonyms: LOC213784, 4833432B22Rik, D4Abb1e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100019
Homologene: 39689
Mmp17
Name: matrix metallopeptidase 17
Synonyms: MT4-MMP, membrane type-4 matrix metalloproteinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 23948
HGNC: HGNC:7163
Homologene: 22669
Sumf1
Name: sulfatase modifying factor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 58911
Homologene: 16268
Uty
Name: ubiquitously transcribed tetratricopeptide repeat containing, Y-linked
Synonyms: Hydb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: Y
NCBI: 22290
Myadm
Name: myeloid-associated differentiation marker
Synonyms: D7Wsu62e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 50918
HGNC: HGNC:7544
Homologene: 9668
Nol11
Name: nucleolar protein 11
Synonyms: 1500002M01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68979
Homologene: 32264
Trpa1
Name: transient receptor potential cation channel, subfamily A, member 1
Synonyms: ANKTM1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 277328
HGNC: HGNC:497
Homologene: 7189
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Trpm5
Name: transient receptor potential cation channel, subfamily M, member 5
Synonyms: Mtr1, Ltrpc5, 9430099A16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56843
Homologene: 22818
Ces2b
Name: carboxyesterase 2B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234669
HGNC: HGNC:1864
Homologene: 135851
Mbl1
Name: mannose-binding lectin (protein A) 1
Synonyms: MBL-A, MBP-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 17194
VEGA: 14
HGNC: HGNC:6921
Homologene: 55449
Caskin2
Name: CASK-interacting protein 2
Synonyms: 1600028L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 140721
Homologene: 32485
Pbld1
Name: phenazine biosynthesis-like protein domain containing 1
Synonyms: 0610038K03Rik, Pbld
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 68371
VEGA: 10
Homologene: 41470
Gpr108
Name: G protein-coupled receptor 108
Synonyms: 1810015L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 78308
VEGA: 17
Homologene: 32495
Teddm1a
Name: transmembrane epididymal protein 1A
Synonyms: e9-1, Epdd1, Teddm1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240819
Homologene: 82370
Atg10
Name: autophagy related 10
Synonyms: 5330424L23Rik, 5430428K15Rik, APG10, Apg10p, Apg10l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66795
VEGA: 13
Homologene: 12036
Ly6f
Name: lymphocyte antigen 6 complex, locus F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 17071
HGNC: HGNC:6728
Homologene: 113718
Tgm2
Name: transglutaminase 2, C polypeptide
Synonyms: tissue transglutaminase, protein-glutamine gamma-glutamyltransferase, G[a]h, TG C, tTGas, tTG, TG2, TGase2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21817
Homologene: 3391
Kcnk18
Name: potassium channel, subfamily K, member 18
Synonyms: LOC332396, Tresk-2, Tresk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 332396
VEGA: 19
Homologene: 133808
Rab36
Name: RAB36, member RAS oncogene family
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 76877
HGNC: HGNC:9775
Homologene: 3610
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 14,885,746 bp
  • A to G, chromosome 1 at 153,891,910 bp
  • C to A, chromosome 2 at 76,900,616 bp
  • C to T, chromosome 2 at 158,124,247 bp
  • T to C, chromosome 4 at 32,750,013 bp
  • T to C, chromosome 5 at 129,595,390 bp
  • G to A, chromosome 6 at 108,153,191 bp
  • A to G, chromosome 7 at 3,297,543 bp
  • G to A, chromosome 7 at 143,085,463 bp
  • A to G, chromosome 8 at 104,834,626 bp
  • T to C, chromosome 10 at 63,071,446 bp
  • G to A, chromosome 10 at 75,044,496 bp
  • A to G, chromosome 11 at 107,173,244 bp
  • A to G, chromosome 11 at 115,806,356 bp
  • A to G, chromosome 13 at 91,040,898 bp
  • C to A, chromosome 14 at 41,158,833 bp
  • T to C, chromosome 15 at 75,268,787 bp
  • A to G, chromosome 17 at 57,245,323 bp
  • T to A, chromosome 19 at 59,235,062 bp
  • G to A, chromosome Y at 1,099,671 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3036 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040552-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.