Strain Name:
C57BL/6J-MtgxR3077Btlr/Mmmh
Stock Number:
040567-MU
Citation ID:
RRID:MMRRC_040567-MU
Other Names:
R3077 (G1), C57BL/6J-MtgxR3077Btlr
Major Collection:

Gene Information

Arhgef7
Name: Rho guanine nucleotide exchange factor (GEF7)
Synonyms: Cool, PIX, Pak interacting exchange factor, p85SPR, betaPix-c, betaPix-b, cool-1, betaPix
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 54126
Homologene: 2895
Shprh
Name: SNF2 histone linker PHD RING helicase
Synonyms: 2610103K11Rik, D230017O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 268281
Homologene: 6489
Abca12
Name: ATP-binding cassette, sub-family A (ABC1), member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74591
Homologene: 45441
Matk
Name: megakaryocyte-associated tyrosine kinase
Synonyms: HYL, CHK, Ntk, Csk homologous kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17179
HGNC: HGNC:6906
Homologene: 48104
Smc3
Name: structural maintenance of chromosomes 3
Synonyms: SmcD, Bamacan, Mmip1, Cspg6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 13006
HGNC: HGNC:2468
Homologene: 3974
Cd79a
Name: CD79A antigen (immunoglobulin-associated alpha)
Synonyms: mb-1, Iga, Ig alpha, Ly54, Cd79a, Ig-alpha, Ly-54, Igalpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12518
HGNC: HGNC:1698
Homologene: 31053
Snap91
Name: synaptosomal-associated protein 91
Synonyms: F1-20, 91kDa, AP180
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20616
Homologene: 8429
Unc45a
Name: unc-45 myosin chaperone A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101869
Homologene: 32423
Dnajc8
Name: DnaJ heat shock protein family (Hsp40) member C8
Synonyms: 1110021D09Rik, 2010009J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 68598
Homologene: 40935
Alms1
Name: ALMS1, centrosome and basal body associated
Synonyms: Alstrom syndrome 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 236266
HGNC: HGNC:428
Homologene: 49406
Mnt
Name: max binding protein
Synonyms: Rox, bHLHd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17428
HGNC: HGNC:7188
Homologene: 7842
Pwwp2a
Name: PWWP domain containing 2A
Synonyms: D930040F23Rik, 4631424J17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70802
Homologene: 19687
Phactr4
Name: phosphatase and actin regulator 4
Synonyms: C330013F19Rik, 3110001B12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100169
Homologene: 41537
Adgrd1
Name: adhesion G protein-coupled receptor D1
Synonyms: E230012M21Rik, Gpr133
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243277
Homologene: 34616
Pcdhb11
Name: protocadherin beta 11
Synonyms: Pcdhb5E, PcdhbK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93882
Homologene: 62178
Pdzd8
Name: PDZ domain containing 8
Synonyms: A630041P07Rik, Pdzk8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107368
VEGA: 19
Homologene: 14879
Trim34b
Name: tripartite motif-containing 34B
Synonyms: Gm15134, Trim34-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434218
Homologene: 128238
Vwa8
Name: von Willebrand factor A domain containing 8
Synonyms: 4932416F07Rik, 1300010F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219189
VEGA: 14
Homologene: 44881
Cabcoco1
Name: ciliary associated calcium binding coiled-coil 1
Synonyms: 1700040L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 73287
Homologene: 12495
Zcchc9
Name: zinc finger, CCHC domain containing 9
Synonyms: 1810019C21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 69085
Homologene: 12999
Olfr389
Name: olfactory receptor 389
Synonyms: GA_x6K02T2P1NL-3932085-3931147, MOR135-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259011
Homologene: 85925
Arfgef3
Name: ARFGEF family member 3
Synonyms: B930094H20Rik, D10Bwg1379e, BIG3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215821
VEGA: 10
Homologene: 41366
Kif14
Name: kinesin family member 14
Synonyms: N-3 kinesin, D1Ertd367e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381293
Homologene: 8916
Zfp628
Name: zinc finger protein 628
Synonyms: Zec
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232816
Homologene: 72200
Cps1
Name: carbamoyl-phosphate synthetase 1
Synonyms: CPS, CPSase I, 4732433M03Rik, D1Ucla3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227231
HGNC: HGNC:2323
Homologene: 68208
Clnk
Name: cytokine-dependent hematopoietic cell linker
Synonyms: MIST
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 27278
Homologene: 8450
Zfp647
Name: zinc finger protein 647
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239546
VEGA: 15
Homologene: 27805
Champ1
Name: chromosome alignment maintaining phosphoprotein 1
Synonyms: D8Ertd569e, D8Ertd457e, Zfp828
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 101994
Homologene: 18780
Olfr576
Name: olfactory receptor 576
Synonyms: GA_x6K02T2PBJ9-5676385-5677371, MOR8-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258248
Homologene: 45856
Nhsl2
Name: NHS-like 2
Synonyms: 1110062M06Rik, Gm10456
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 100042480
Homologene: 19759
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 67,143,970 bp
  • T to C, chromosome 1 at 71,267,605 bp
  • T to G, chromosome 1 at 136,519,645 bp
  • A to G, chromosome 4 at 132,397,996 bp
  • A to G, chromosome 4 at 132,544,663 bp
  • C to T, chromosome 5 at 38,715,028 bp
  • A to T, chromosome 5 at 129,129,105 bp
  • C to A, chromosome 6 at 85,588,035 bp
  • A to G, chromosome 7 at 4,921,200 bp
  • A to G, chromosome 7 at 24,901,308 bp
  • A to C, chromosome 7 at 80,338,932 bp
  • G to T, chromosome 7 at 102,966,016 bp
  • A to G, chromosome 7 at 104,331,301 bp
  • T to A, chromosome 8 at 11,815,026 bp
  • T to C, chromosome 8 at 13,878,832 bp
  • T to A, chromosome 9 at 86,838,854 bp
  • T to C, chromosome 10 at 11,170,413 bp
  • T to C, chromosome 10 at 18,603,530 bp
  • T to G, chromosome 10 at 68,525,645 bp
  • A to G, chromosome 10 at 81,259,379 bp
  • A to G, chromosome 11 at 43,705,385 bp
  • G to A, chromosome 11 at 73,776,640 bp
  • G to C, chromosome 11 at 74,843,110 bp
  • A to T, chromosome 13 at 91,805,982 bp
  • A to T, chromosome 14 at 79,098,342 bp
  • A to T, chromosome 15 at 76,918,009 bp
  • C to T, chromosome 18 at 37,422,244 bp
  • A to G, chromosome 19 at 53,627,891 bp
  • A to G, chromosome 19 at 59,305,156 bp
  • C to T, chromosome X at 102,077,595 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3077 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040567-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.