Strain Name:
C57BL/6J-MtgxR3086Btlr/Mmmh
Stock Number:
040575-MU
Citation ID:
RRID:MMRRC_040575-MU
Other Names:
R3086 (G1), C57BL/6J-MtgxR3086Btlr
Major Collection:

Gene Information

Kifc2
Name: kinesin family member C2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16581
VEGA: 15
Homologene: 7800
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11426
Homologene: 136191
Tet1
Name: tet methylcytosine dioxygenase 1
Synonyms: 2510010B09Rik, D10Ertd17e, Cxxc6, BB001228
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 52463
Homologene: 12735
Nf1
Name: neurofibromin 1
Synonyms: neurofibromin, Nf-1, Dsk9, Mhdadsk9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Orc4
Name: origin recognition complex, subunit 4
Synonyms: Orc4P, mMmORC4, Orc4, Orc4l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 26428
HGNC: HGNC:8490
Homologene: 8059
Kif20b
Name: kinesin family member 20B
Synonyms: N-6 kinesin, C330014J10Rik, Kif20b, Mphosph1, 33cex, magoo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 240641
VEGA: 19
HGNC: HGNC:7212
Homologene: 9418
4932438A13Rik
Name: RIKEN cDNA 4932438A13 gene
Synonyms: Tweek, FSA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229227
Homologene: 52105
Dock4
Name: dedicator of cytokinesis 4
Synonyms: EST N28122, 6330411N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238130
VEGA: 12
Homologene: 56680
Tep1
Name: telomerase associated protein 1
Synonyms: Tp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21745
VEGA: 14
Homologene: 5157
N4bp2
Name: NEDD4 binding protein 2
Synonyms: LOC333789, LOC386488, B3bp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 333789
Homologene: 32396
Frzb
Name: frizzled-related protein
Synonyms: fritz, frzb-1, Sfrp3, Frp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20378
HGNC: HGNC:3959
Homologene: 1126
Alms1
Name: ALMS1, centrosome and basal body associated
Synonyms: Alstrom syndrome 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 236266
HGNC: HGNC:428
Homologene: 49406
Helq
Name: helicase, POLQ-like
Synonyms: D430018E21Rik, Hel308
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 191578
Homologene: 14667
Prune2
Name: prune homolog 2
Synonyms: 6330414G02Rik, A330102H22Rik, A230083H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 353211
VEGA: 19
Homologene: 18939
Adgra3
Name: adhesion G protein-coupled receptor A3
Synonyms: 3830613O22Rik, Tem5-like, Gpr125
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 70693
Homologene: 19235
Tmem68
Name: transmembrane protein 68
Synonyms: 2010300G19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 72098
Homologene: 12440
Zfyve26
Name: zinc finger, FYVE domain containing 26
Synonyms: 9330197E15Rik, LOC380767, A630028O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 211978
VEGA: 12
Homologene: 9102
Tmem131l
Name: transmembrane 131 like
Synonyms: D930015E06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229473
Homologene: 9057
Fgfr4
Name: fibroblast growth factor receptor 4
Synonyms: Fgfr-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 14186
HGNC: HGNC:3691
Homologene: 20461
Dock10
Name: dedicator of cytokinesis 10
Synonyms: Jr5, Jr4, ZIZ3, 9330153B10Rik, A630054M16Rik, Zizimin3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210293
Homologene: 45952
Megf8
Name: multiple EGF-like-domains 8
Synonyms: Egfl4, b2b288Clo, b2b1702Clo, m687Ddg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269878
HGNC: HGNC:3233
Homologene: 15988
Olfr1280
Name: olfactory receptor 1280
Synonyms: GA_x6K02T2Q125-72366920-72367837, MOR248-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258910
Homologene: 121540
Stk-ps2
Name: serine/threonine kinase 2
Synonyms: Gm4776
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 212225
Rsph4a
Name: radial spoke head 4 homolog A (Chlamydomonas)
Synonyms: Rshl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 212892
Homologene: 71779
Plekhh1
Name: pleckstrin homology domain containing, family H (with MyTH4 domain) member 1
Synonyms: D630024D12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 211945
VEGA: 12
Homologene: 121939
Ces2b
Name: carboxyesterase 2B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234669
HGNC: HGNC:1864
Homologene: 135851
Sema5b
Name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B
Synonyms: SemG, SemG, Semag
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 20357
Homologene: 8427
Dlg5
Name: discs large MAGUK scaffold protein 5
Synonyms: 4933429D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71228
HGNC: HGNC:2904
Homologene: 3486
Dhrs2
Name: dehydrogenase/reductase member 2
Synonyms: SDR family, 5430405K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71412
Homologene: 69438
Tiam2
Name: T cell lymphoma invasion and metastasis 2
Synonyms: STEF, 3000002F19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 24001
VEGA: 17
Homologene: 40796
Frk
Name: fyn-related kinase
Synonyms: BSK/IYK, GTK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 14302
VEGA: 10
HGNC: HGNC:3955
Homologene: 48065
Vmn2r101
Name: vomeronasal 2, receptor 101
Synonyms: EG627576
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 627576
Homologene: 115024
Zfp780b
Name: zinc finger protein 780B
Synonyms: B230208L21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 338354
Homologene: 85969
Gsg1l
Name: GSG1-like
Synonyms: G630023A01Rik, C230098I05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269994
Homologene: 78152
Cabyr
Name: calcium-binding tyrosine-(Y)-phosphorylation regulated (fibrousheathin 2)
Synonyms: FSP-2, CBP86, 4933421A18Rik, 1700016C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 71132
Homologene: 49299
D330041H03Rik
Name: RIKEN cDNA D330041H03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 654822
Olfr794
Name: olfactory receptor 794
Synonyms: GA_x6K02T2PULF-11248702-11249664, MOR114-11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258375
Homologene: 45066
Lekr1
Name: leucine, glutamate and lysine rich 1
Synonyms: EG546798, Gm6534
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 624866
Homologene: 54497
Acot8
Name: acyl-CoA thioesterase 8
Synonyms: PTE-2, Pte1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 170789
Homologene: 3991
Olfr1313
Name: olfactory receptor 1313
Synonyms: GA_x6K02T2Q125-73119859-73118924, MOR245-23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258257
Homologene: 106834
Rbp2
Name: retinol binding protein 2, cellular
Synonyms: Crbp-2, Rbp-2, CrbpII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19660
HGNC: HGNC:9920
Homologene: 3070
2310039H08Rik
Name: RIKEN cDNA 2310039H08 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 67101
VEGA: 17
Homologene: 87029
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 46,029,076 bp
  • T to C, chromosome 1 at 80,532,357 bp
  • G to A, chromosome 2 at 48,937,489 bp
  • T to G, chromosome 2 at 80,418,514 bp
  • A to G, chromosome 2 at 111,316,116 bp
  • C to A, chromosome 2 at 112,071,975 bp
  • G to A, chromosome 2 at 164,804,684 bp
  • A to G, chromosome 3 at 37,011,703 bp
  • A to G, chromosome 3 at 65,727,160 bp
  • G to T, chromosome 3 at 83,931,739 bp
  • T to C, chromosome 4 at 3,569,594 bp
  • T to A, chromosome 4 at 123,435,108 bp
  • A to T, chromosome 5 at 50,013,391 bp
  • A to G, chromosome 5 at 65,791,053 bp
  • A to G, chromosome 5 at 100,773,992 bp
  • A to G, chromosome 6 at 85,678,140 bp
  • T to A, chromosome 7 at 25,349,019 bp
  • A to G, chromosome 7 at 27,963,630 bp
  • C to T, chromosome 7 at 125,891,680 bp
  • A to T, chromosome 8 at 104,832,769 bp
  • T to G, chromosome 9 at 98,507,778 bp
  • G to C, chromosome 10 at 33,909,202 bp
  • G to A, chromosome 10 at 34,607,954 bp
  • T to C, chromosome 10 at 62,879,621 bp
  • G to A, chromosome 10 at 129,571,407 bp
  • T to C, chromosome 11 at 79,546,986 bp
  • T to C, chromosome 12 at 40,731,863 bp
  • T to C, chromosome 12 at 79,070,779 bp
  • G to T, chromosome 12 at 79,265,683 bp
  • G to T, chromosome 13 at 55,167,392 bp
  • T to C, chromosome 14 at 24,166,190 bp
  • A to T, chromosome 14 at 50,827,054 bp
  • G to T, chromosome 14 at 55,239,844 bp
  • T to C, chromosome 15 at 76,667,252 bp
  • T to A, chromosome 16 at 35,622,723 bp
  • A to G, chromosome 17 at 3,421,582 bp
  • T to A, chromosome 17 at 19,588,815 bp
  • C to T, chromosome 17 at 24,412,419 bp
  • T to C, chromosome 17 at 46,772,955 bp
  • T to C, chromosome 18 at 12,750,966 bp
  • A to T, chromosome 19 at 17,121,413 bp
  • T to C, chromosome 19 at 34,929,715 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3086 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040575-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.