Strain Name:
C57BL/6J-MtgxR3109Btlr/Mmmh
Stock Number:
040583-MU
Citation ID:
RRID:MMRRC_040583-MU
Other Names:
R3109 (G1), C57BL/6J-MtgxR3109Btlr
Major Collection:

Gene Information

Adamts20
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223838
Homologene: 11808
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231051
Homologene: 46480
Drd4
Name: dopamine receptor D4
Synonyms: Drd-4, D4R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13491
HGNC: HGNC:3025
Homologene: 20215
Ntrk3
Name: neurotrophic tyrosine kinase, receptor, type 3
Synonyms: TrkC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18213
HGNC: HGNC:8033
Homologene: 49183
Camk1g
Name: calcium/calmodulin-dependent protein kinase I gamma
Synonyms: CLICK-III, CaMKIgamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 215303
Homologene: 48706
Klhdc4
Name: kelch domain containing 4
Synonyms: G430025P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234825
Homologene: 69234
Arid2
Name: AT rich interactive domain 2 (ARID, RFX-like)
Synonyms: 4432409D24Rik, zipzap/p200, 1700124K17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 77044
Homologene: 14601
Cnot1
Name: CCR4-NOT transcription complex, subunit 1
Synonyms: 6030411K04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234594
HGNC: HGNC:7877
Homologene: 9453
Dclk2
Name: doublecortin-like kinase 2
Synonyms: 6330415M09Rik, Click-II, Dcamkl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 70762
Homologene: 69431
Unc45a
Name: unc-45 myosin chaperone A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101869
Homologene: 32423
Dennd1b
Name: DENN/MADD domain containing 1B
Synonyms: F730008N07Rik, 4632404N19Rik, 4930467M19Rik, 6820401H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329260
Homologene: 11739
Ubr3
Name: ubiquitin protein ligase E3 component n-recognin 3
Synonyms: 4833421P10Rik, A130030D10Rik, 1110059H15Rik, Zfp650
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68795
Homologene: 52092
Rbl2
Name: RB transcriptional corepressor like 2
Synonyms: Rb2, p130, retinoblastoma-like 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 19651
HGNC: HGNC:9894
Homologene: 4098
Fat1
Name: FAT atypical cadherin 1
Synonyms: mFat1, 2310038E12Rik, Fath
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 14107
HGNC: HGNC:3595
Homologene: 66302
Zfp708
Name: zinc finger protein 708
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 432769
Homologene: 134096
Amfr
Name: autocrine motility factor receptor
Synonyms: gp78
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 23802
HGNC: HGNC:463
Homologene: 888
Cubn
Name: cubilin (intrinsic factor-cobalamin receptor)
Synonyms: D2Wsu88e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 65969
HGNC: HGNC:2548
Homologene: 37434
Lama2
Name: laminin, alpha 2
Synonyms: merosin, mer
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16773
HGNC: HGNC:6482
Homologene: 37306
Map3k5
Name: mitogen-activated protein kinase kinase kinase 5
Synonyms: ASK1, Mekk5, 7420452D20Rik, ASK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 26408
HGNC: HGNC:6857
Homologene: 38114
Per2
Name: period circadian clock 2
Synonyms: mPer2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18627
HGNC: HGNC:8846
Homologene: 7885
Muc5b
Name: mucin 5, subtype B, tracheobronchial
Synonyms: MUC9, MUC5, 2300002I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74180
HGNC: HGNC:7516
Homologene: 136756
Ptprn2
Name: protein tyrosine phosphatase, receptor type, N polypeptide 2
Synonyms: PTP-NP, IA-2 beta, phogrin, IA-2beta, 4930425H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 19276
HGNC: HGNC:9677
Homologene: 2134
Or52s1b
Name: olfactory receptor family 52 subfamily S member 1B
Synonyms: GA_x6K02T2PBJ9-5889409-5888465, MOR24-1P, Olfr591
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258139
Homologene: 79429
Fyn
Name: Fyn proto-oncogene
Synonyms: Src Kinase p59
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 14360
HGNC: HGNC:4037
Homologene: 48068
Dmrt2
Name: doublesex and mab-3 related transcription factor 2
Synonyms: Terra
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226049
VEGA: 19
HGNC: HGNC:2935
Homologene: 17111
Igfn1
Name: immunoglobulin-like and fibronectin type III domain containing 1
Synonyms: 9830123M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226438
Homologene: 130054
Sgsm2
Name: small G protein signaling modulator 2
Synonyms: D630003G22Rik, Rutbc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 97761
Homologene: 14780
Cnnm1
Name: cyclin M1
Synonyms: Acdp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 83674
VEGA: 19
HGNC: HGNC:102
Homologene: 10673
Alkbh3
Name: alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase
Synonyms: 1700108H04Rik, 1810020C19Rik, Abh3, mABH3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 69113
Homologene: 16393
6820408C15Rik
Name: RIKEN cDNA 6820408C15 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228778
Homologene: 51916
Trim30d
Name: tripartite motif-containing 30D
Synonyms: TRIM30-3, Trim79
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 209387
Homologene: 114426
Vmn1r175
Name: vomeronasal 1 receptor 175
Synonyms: Gm6299
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 622222
Homologene: 128341
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 91,445,575 bp
  • T to C, chromosome 1 at 135,997,848 bp
  • G to A, chromosome 1 at 139,041,916 bp
  • T to C, chromosome 1 at 193,354,993 bp
  • T to C, chromosome 2 at 13,362,347 bp
  • A to T, chromosome 2 at 69,988,840 bp
  • T to C, chromosome 2 at 94,004,763 bp
  • A to G, chromosome 2 at 152,442,456 bp
  • G to A, chromosome 3 at 86,920,035 bp
  • A to G, chromosome 5 at 25,275,735 bp
  • A to G, chromosome 7 at 23,808,968 bp
  • C to T, chromosome 7 at 78,460,515 bp
  • A to G, chromosome 7 at 80,331,546 bp
  • T to A, chromosome 7 at 103,173,086 bp
  • A to T, chromosome 7 at 104,473,293 bp
  • T to A, chromosome 7 at 141,292,282 bp
  • C to T, chromosome 7 at 141,858,759 bp
  • G to A, chromosome 8 at 45,045,173 bp
  • T to A, chromosome 8 at 91,102,235 bp
  • T to C, chromosome 8 at 94,000,306 bp
  • A to G, chromosome 8 at 95,735,749 bp
  • A to C, chromosome 8 at 121,821,334 bp
  • T to A, chromosome 10 at 20,131,952 bp
  • C to T, chromosome 10 at 27,001,235 bp
  • G to C, chromosome 10 at 39,551,455 bp
  • A to G, chromosome 11 at 74,869,186 bp
  • A to G, chromosome 12 at 116,876,180 bp
  • C to T, chromosome 13 at 67,098,607 bp
  • T to C, chromosome 15 at 94,345,904 bp
  • T to C, chromosome 15 at 96,356,746 bp
  • C to A, chromosome 19 at 25,677,691 bp
  • T to C, chromosome 19 at 43,441,561 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3109 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040583-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.