Strain Name:
C57BL/6J-MtgxR3114Btlr/Mmmh
Stock Number:
040587-MU
Citation ID:
RRID:MMRRC_040587-MU
Other Names:
R3114 (G1), C57BL/6J-MtgxR3114Btlr
Major Collection:

Strain Information

Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: PRCE, Cerp, PRCE, SSE, ESP1, separase
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Chrna3
Name: cholinergic receptor, nicotinic, alpha polypeptide 3
Synonyms: Acra3, Acra-3, (a)3, alpha 3, neuronal nicotinic acetylcholine receptor, alpha 3 subunit, A730007P14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110834
VEGA: 9
HGNC: HGNC:1957
Homologene: 591
Mier3
Name: MIER family member 3
Synonyms: D130064H19Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218613
Homologene: 18196
Rnmt
Name: RNA (guanine-7-) methyltransferase
Synonyms: 2610002P10Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67897
Homologene: 2816
Pds5a
Name: PDS5 cohesin associated factor A
Synonyms: 9030416H16Rik, E230024D05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71521
Homologene: 22877
Tsc22d1
Name: TSC22 domain family, member 1
Synonyms: Egr5, Tgfb1i4, TSC-22
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 21807
Homologene: 7573
Cnot1
Name: CCR4-NOT transcription complex, subunit 1
Synonyms: 6030411K04Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234594
HGNC: HGNC:7877
Homologene: 9453
Sspn
Name: sarcospan
Synonyms: Krag
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16651
Homologene: 3727
Mei1
Name: meiotic double-stranded break formation protein 1
Synonyms: mei1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 74369
Homologene: 46535
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Hst6.7b, Dnahc8, P1-Loop
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Emc1
Name: ER membrane protein complex subunit 1
Synonyms: C230096C10Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230866
Homologene: 9002
Fzd5
Name: frizzled class receptor 5
Synonyms: Fz5, 5330434N09Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14367
HGNC: HGNC:4043
Homologene: 2590
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 77505
Homologene: 131117
Pde4d
Name: phosphodiesterase 4D, cAMP specific
Synonyms: Dpde3, 9630011N22Rik, dunce
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 238871
HGNC: HGNC:8783
Nos3
Name: nitric oxide synthase 3, endothelial cell
Synonyms: 2310065A03Rik, ecNOS, Nos-3, eNOS
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18127
HGNC: HGNC:7876
Homologene: 504
Mlxipl
Name: MLX interacting protein-like
Synonyms: bHLHd14, WS-bHLH, Wbscr14, ChREBP
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 58805
Homologene: 32507
Cdt1
Name: chromatin licensing and DNA replication factor 1
Synonyms: Ris2, 2610318F11Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67177
Homologene: 32650
Wdfy4
Name: WD repeat and FYVE domain containing 4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 545030
Homologene: 83473
H2-M10.6
Name: histocompatibility 2, M region locus 10.6
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 399549
Homologene: 117973
Sik1
Name: salt inducible kinase 1
Synonyms: Msk, Snf1lk
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17691
VEGA: 17
Homologene: 7847
Slfn10-ps
Name: schlafen 10, pseudogene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237887
Rint1
Name: RAD50 interactor 1
Synonyms: 1500019C06Rik, 2810450M21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72772
Homologene: 11070
Cfap65
Name: cilia and flagella associated protein 65
Synonyms: Ccdc108, B230363K08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241116
Homologene: 28093
Meak7
Name: MTOR associated protein, eak-7 homolog
Synonyms: 4632415K11Rik, Tldc1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74347
Homologene: 32494
Krt24
Name: keratin 24
Synonyms: 2310075C18Rik, 2310058N18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 75706
Homologene: 10379
Ptar1
Name: protein prenyltransferase alpha subunit repeat containing 1
Synonyms: 4930428J16Rik, 2010013L17Rik, 1700084D09Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 72351
VEGA: 19
Homologene: 34254
Or4a2
Name: olfactory receptor family 4 subfamily A member 2
Synonyms: Olfr1239, GA_x6K02T2Q125-50861284-50860367, MOR231-3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258972
Homologene: 74244
Serpinb8
Name: serine (or cysteine) peptidase inhibitor, clade B, member 8
Synonyms: Spi8, NK10, ovalbumin, CAP-2, CAP2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20725
HGNC: HGNC:8952
Homologene: 74445
Klhl33
Name: kelch-like 33
Synonyms: EG546611
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 546611
VEGA: 14
Homologene: 52385
Mob3a
Name: MOB kinase activator 3A
Synonyms: Mobkl2a, 5330417K06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 208228
VEGA: 10
Homologene: 41587
Marchf1
Name: membrane associated ring-CH-type finger 1
Synonyms: 2900024D24Rik, March1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 72925
Homologene: 113749
Esp36
Name: exocrine gland secreted peptide 36
Synonyms: Gm20408
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 100126765
Klhl3
Name: kelch-like 3
Synonyms: EG627648, 7530408C15Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 100503085
HGNC: HGNC:6354
Homologene: 79542
Gm1587
Name: predicted gene 1587
Synonyms: LOC380920
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 380920
VEGA: 14
H3c3
Name: H3 clustered histone 3
Synonyms: H3-143, Hist1h3c
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 319148
Homologene: 134489
Spmip8
Name: sperm microtubule inner protein 8
Synonyms: 1700055M20Rik, Tepp
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 73407
Homologene: 28311
Ighv5-2
Name: immunoglobulin heavy variable 5-2
Synonyms: Gm16969
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 777685
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 64,735,580 bp
  • T to A, chromosome 1 at 74,927,132 bp
  • A to G, chromosome 1 at 107,607,293 bp
  • T to A, chromosome 2 at 89,418,413 bp
  • T to C, chromosome 4 at 139,360,447 bp
  • A to G, chromosome 5 at 23,819,420 bp
  • C to T, chromosome 5 at 24,372,631 bp
  • A to G, chromosome 5 at 65,618,985 bp
  • A to G, chromosome 5 at 135,133,662 bp
  • A to T, chromosome 6 at 145,934,369 bp
  • T to C, chromosome 7 at 105,696,565 bp
  • A to C, chromosome 8 at 66,387,381 bp
  • T to C, chromosome 8 at 95,313,181 bp
  • T to C, chromosome 8 at 95,744,278 bp
  • G to A, chromosome 8 at 119,768,317 bp
  • C to T, chromosome 8 at 122,570,482 bp
  • C to T, chromosome 9 at 55,016,050 bp
  • A to T, chromosome 10 at 80,691,302 bp
  • T to A, chromosome 11 at 83,029,129 bp
  • T to A, chromosome 11 at 99,282,436 bp
  • A to G, chromosome 12 at 113,574,224 bp
  • C to A, chromosome 13 at 23,745,307 bp
  • A to G, chromosome 13 at 58,051,027 bp
  • T to A, chromosome 13 at 109,948,258 bp
  • T to A, chromosome 13 at 111,706,648 bp
  • A to G, chromosome 14 at 33,089,903 bp
  • A to T, chromosome 14 at 50,891,515 bp
  • T to C, chromosome 14 at 76,417,337 bp
  • T to G, chromosome 14 at 77,798,832 bp
  • C to T, chromosome 15 at 82,124,959 bp
  • T to A, chromosome 15 at 102,323,204 bp
  • A to G, chromosome 17 at 30,833,568 bp
  • T to C, chromosome 17 at 31,848,132 bp
  • T to C, chromosome 17 at 36,814,099 bp
  • T to C, chromosome 17 at 38,419,535 bp
  • A to G, chromosome 18 at 68,314,008 bp
  • T to A, chromosome 19 at 23,718,095 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3114 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040587-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.