Strain Name:
C57BL/6J-MtgxR3148Btlr/Mmmh
Stock Number:
040600-MU
Citation ID:
RRID:MMRRC_040600-MU
Other Names:
R3148 (G1), C57BL/6J-MtgxR3148Btlr
Major Collection:

Gene Information

Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Prrx1
Name: paired related homeobox 1
Synonyms: K-2, Prx1, mHox, Pmx1, mHox, A230024N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18933
HGNC: HGNC:9142
Homologene: 7896
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Pcbp1
Name: poly(rC) binding protein 1
Synonyms: [a]CP-1, WBP17, hnRNP E1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 23983
HGNC: HGNC:8647
Homologene: 68506
Snx4
Name: sorting nexin 4
Synonyms: 1810036H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 69150
VEGA: 16
Homologene: 36143
Marf1
Name: meiosis regulator and mRNA stability 1
Synonyms: 4921513D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 223989
VEGA: 16
Homologene: 40967
Fntb
Name: farnesyltransferase, CAAX box, beta
Synonyms: 2010013E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 110606
HGNC: HGNC:3785
Homologene: 1535
Cc2d2a
Name: coiled-coil and C2 domain containing 2A
Synonyms: 5730509K17Rik, b2b1035Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231214
Homologene: 18159
Alg2
Name: asparagine-linked glycosylation 2 (alpha-1,3-mannosyltransferase)
Synonyms: ALPG2, CDGIi, 1300013N08Rik, 1110018A23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56737
Homologene: 5930
Sqstm1
Name: sequestosome 1
Synonyms: A170, OSF-6, STAP, Osi, p62
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18412
Homologene: 31202
Trmt10b
Name: tRNA methyltransferase 10B
Synonyms: 2610042J10Rik, Rg9mtd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 69934
Homologene: 12340
Ehbp1
Name: EH domain binding protein 1
Synonyms: Flj21950, KIAA0903-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216565
Homologene: 22880
Kcnj11
Name: potassium inwardly rectifying channel, subfamily J, member 11
Synonyms: Kir6.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16514
HGNC: HGNC:6257
Homologene: 441
Col7a1
Name: collagen, type VII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12836
HGNC: HGNC:2214
Homologene: 73
Trpm1
Name: transient receptor potential cation channel, subfamily M, member 1
Synonyms: Mlsn1, 4732499L03Rik, melastatin, LTRPC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17364
HGNC: HGNC:7146
Homologene: 19940
Cntnap4
Name: contactin associated protein-like 4
Synonyms: Caspr4, E130114F09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 170571
Homologene: 24912
Otog
Name: otogelin
Synonyms: Otgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18419
HGNC: HGNC:8516
Homologene: 8421
Baalc
Name: brain and acute leukemia, cytoplasmic
Synonyms: 2810457D07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 118452
VEGA: 15
Homologene: 11732
Olfr1280
Name: olfactory receptor 1280
Synonyms: GA_x6K02T2Q125-72366920-72367837, MOR248-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258910
Homologene: 121540
Pam
Name: peptidylglycine alpha-amidating monooxygenase
Synonyms: PHM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18484
HGNC: HGNC:8596
Homologene: 37369
Spata16
Name: spermatogenesis associated 16
Synonyms: spermatogenesis-related protein, Nyd-sp12, 4930503K02Rik, 4921511F01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 70862
Homologene: 49974
Cps1
Name: carbamoyl-phosphate synthetase 1
Synonyms: CPS, CPSase I, 4732433M03Rik, D1Ucla3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227231
HGNC: HGNC:2323
Homologene: 68208
Adamts18
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 18
Synonyms: E130314N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 208936
Homologene: 65241
Pramel21
Name: PRAME like 21
Synonyms: Gm13083
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 279185
Homologene: 133722
Asb15
Name: ankyrin repeat and SOCS box-containing 15
Synonyms: 4930400E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 78910
Homologene: 43797
Sorcs2
Name: sortilin-related VPS10 domain containing receptor 2
Synonyms: VPS10 domain receptor protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 81840
Homologene: 56899
Klk14
Name: kallikrein related-peptidase 14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 317653
HGNC: HGNC:6362
Homologene: 69348
Tcerg1l
Name: transcription elongation regulator 1-like
Synonyms: 5730476P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 70571
Homologene: 52165
Catsperd
Name: cation channel sperm associated auxiliary subunit delta
Synonyms: 4933402B14Rik, Gm6095, 4921529N20Rik, Tmem146
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106757
VEGA: 17
Homologene: 51896
Serpinb5
Name: serine (or cysteine) peptidase inhibitor, clade B, member 5
Synonyms: Maspin, Spi7, ovalbumin, 1110036M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20724
HGNC: HGNC:8949
Homologene: 20580
4933436I01Rik
Name: RIKEN cDNA 4933436I01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 66780
Homologene: 12034
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,299,707 bp
  • C to T, chromosome 1 at 67,177,100 bp
  • T to A, chromosome 1 at 97,895,678 bp
  • T to A, chromosome 1 at 106,881,825 bp
  • T to C, chromosome 1 at 163,257,848 bp
  • T to C, chromosome 2 at 111,316,288 bp
  • T to C, chromosome 3 at 26,878,712 bp
  • T to C, chromosome 3 at 126,933,075 bp
  • A to G, chromosome 4 at 45,304,402 bp
  • A to T, chromosome 4 at 47,472,259 bp
  • A to T, chromosome 4 at 143,617,477 bp
  • T to C, chromosome 5 at 36,035,788 bp
  • T to A, chromosome 5 at 43,709,155 bp
  • G to T, chromosome 6 at 24,566,259 bp
  • C to T, chromosome 6 at 86,525,489 bp
  • G to A, chromosome 7 at 43,692,077 bp
  • C to T, chromosome 7 at 46,099,120 bp
  • T to C, chromosome 7 at 46,290,169 bp
  • T to C, chromosome 7 at 64,235,012 bp
  • G to T, chromosome 7 at 138,259,867 bp
  • A to G, chromosome 8 at 112,757,439 bp
  • C to A, chromosome 8 at 113,738,858 bp
  • A to G, chromosome 9 at 108,961,405 bp
  • T to C, chromosome 11 at 22,100,465 bp
  • A to G, chromosome 11 at 50,206,111 bp
  • C to A, chromosome 12 at 76,887,888 bp
  • A to T, chromosome 15 at 38,949,173 bp
  • A to G, chromosome 16 at 14,125,979 bp
  • A to C, chromosome 16 at 33,287,724 bp
  • G to T, chromosome 17 at 56,664,039 bp
  • T to C, chromosome X at 67,921,378 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3148 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040600-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.