Strain Name:
C57BL/6J-MtgxR3149Btlr/Mmmh
Stock Number:
040601-MU
Citation ID:
RRID:MMRRC_040601-MU
Other Names:
R3149 (G1), C57BL/6J-MtgxR3149Btlr
Major Collection:

Gene Information

Atox1
Name: antioxidant 1 copper chaperone
Synonyms: ATX1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11927
HGNC: HGNC:798
Homologene: 2984
Pecam1
Name: platelet/endothelial cell adhesion molecule 1
Synonyms: Cd31, PECAM-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18613
HGNC: HGNC:8823
Homologene: 47925
Ggta1
Name: glycoprotein galactosyltransferase alpha 1, 3
Synonyms: glycoprotein alpha galactosyl transferase 1, Ggta-1, GALT, alpha3GalT, Ggta, Gal, alpha Gal
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14594
HGNC: HGNC:4253
Homologene: 7730
Srgap2
Name: SLIT-ROBO Rho GTPase activating protein 2
Synonyms: Fnbp2, FBP2, 9930124L22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14270
Homologene: 52683
Vps13c
Name: vacuolar protein sorting 13C
Synonyms: C230055H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320528
VEGA: 9
Homologene: 41188
Rock2
Name: Rho-associated coiled-coil containing protein kinase 2
Synonyms: B230113H15Rik, Rho-kinase, ROKalpha, Rock-II, Rock2m
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 19878
VEGA: 12
Homologene: 21010
Gpatch2l
Name: G patch domain containing 2 like
Synonyms: 1700020O03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 70373
VEGA: 12
Homologene: 9942
Brca1
Name: breast cancer 1, early onset
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12189
HGNC: HGNC:1100
Homologene: 5276
Mettl25
Name: methyltransferase like 25
Synonyms: BC067068
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216292
Homologene: 32774
Tasor2
Name: transcription activation suppressor family member 2
Synonyms: BC016423, Fam208b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105203
VEGA: 13
Homologene: 26435
Xpo5
Name: exportin 5
Synonyms: 2700038C24Rik, Exp5, 2410004H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72322
VEGA: 17
Homologene: 69316
Vps13d
Name: vacuolar protein sorting 13D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230895
Homologene: 15583
Kirrel
Name: kirre like nephrin family adhesion molecule 1
Synonyms: Neph1, Kirrel1, 6720469N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 170643
Homologene: 10089
Cyp4f18
Name: cytochrome P450, family 4, subfamily f, polypeptide 18
Synonyms: 1810054N16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72054
HGNC: HGNC:2645
Homologene: 128623
Hoxa13
Name: homeobox A13
Synonyms: Hox-1.10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 15398
HGNC: HGNC:5102
Homologene: 73882
Mapk11
Name: mitogen-activated protein kinase 11
Synonyms: p38beta, Prkm11, P38b, Sapk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 19094
VEGA: 15
HGNC: HGNC:6873
Homologene: 55684
Rmnd5a
Name: required for meiotic nuclear division 5 homolog A
Synonyms: 1110007A06Rik, Gid2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68477
Homologene: 5668
Gm11492
Name: predicted gene 11492
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Csf2ra
Name: colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
Synonyms: CD116, GM-CSF-Ra, GM-CSFRalpha, Csfgmra
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 12982
VEGA: 19
HGNC: HGNC:2435
Homologene: 48406
Dus1l
Name: dihydrouridine synthase 1-like (S. cerevisiae)
Synonyms: 1110032N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68730
Homologene: 6569
Acsbg3
Name: acyl-CoA synthetase bubblegum family member 3
Synonyms: 1700061G19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 78625
Homologene: 28378
Plxnc1
Name: plexin C1
Synonyms: 2510048K12Rik, vespr, CD232
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 54712
HGNC: HGNC:9106
Homologene: 4211
Mrc2
Name: mannose receptor, C type 2
Synonyms: uPARAP, Endo180, novel lectin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17534
Homologene: 4408
Vmn2r68
Name: vomeronasal 2, receptor 68
Synonyms: EG620697, Vmn2r68-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 620697
Homologene: 115466
Cel
Name: carboxyl ester lipase
Synonyms: BAL, 1810036E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12613
HGNC: HGNC:1848
Homologene: 37529
Vmn1r86
Name: vomeronasal 1 receptor 86
Synonyms: Gm10301
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100312473
Homologene: 74345
Dzip1
Name: DAZ interacting protein 1
Synonyms: 2810422M04Rik, 2510025K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66573
VEGA: 14
Homologene: 45708
Rassf9
Name: Ras association (RalGDS/AF-6) domain family (N-terminal) member 9
Synonyms: Pamci
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237504
Homologene: 3976
Dgki
Name: diacylglycerol kinase, iota
Synonyms: C130010K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320127
HGNC: HGNC:2855
Homologene: 37956
Olfr479
Name: olfactory receptor 479
Synonyms: GA_x6K02T2PBJ9-10384085-10385068, MOR267-15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 257891
Homologene: 79346
AC153874.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Zswim9
Name: zinc finger SWIM-type containing 9
Synonyms: 6330408A02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 321008
Homologene: 52386
Gm5592
Name: predicted gene 5592
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434172
Homologene: 45597
Mtmr11
Name: myotubularin related protein 11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 194126
Homologene: 82382
Gm5150
Name: predicted gene 5150
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 381484
Homologene: 129516
Prkx
Name: protein kinase, X-linked
Synonyms: Pkare
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 19108
HGNC: HGNC:9441
Homologene: 55868
Ift46
Name: intraflagellar transport 46
Synonyms: IFT46, 1500035H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 76568
Homologene: 10596
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 131,292,589 bp
  • T to C, chromosome 2 at 28,556,131 bp
  • A to T, chromosome 2 at 35,402,623 bp
  • A to G, chromosome 3 at 16,006,315 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • T to C, chromosome 3 at 96,167,891 bp
  • G to C, chromosome 4 at 145,126,577 bp
  • A to T, chromosome 6 at 36,847,499 bp
  • G to T, chromosome 6 at 52,260,304 bp
  • T to A, chromosome 6 at 71,429,101 bp
  • T to A, chromosome 7 at 13,102,431 bp
  • T to C, chromosome 7 at 13,277,270 bp
  • A to G, chromosome 7 at 41,288,380 bp
  • A to C, chromosome 7 at 85,237,667 bp
  • C to T, chromosome 7 at 108,055,782 bp
  • T to C, chromosome 8 at 71,993,200 bp
  • A to G, chromosome 9 at 44,783,748 bp
  • T to C, chromosome 9 at 67,948,108 bp
  • T to C, chromosome 10 at 77,788,005 bp
  • C to G, chromosome 10 at 94,799,259 bp
  • A to T, chromosome 10 at 102,544,826 bp
  • T to C, chromosome 10 at 105,826,353 bp
  • A to G, chromosome 11 at 55,450,553 bp
  • T to C, chromosome 11 at 87,567,244 bp
  • A to G, chromosome 11 at 101,532,012 bp
  • G to A, chromosome 11 at 105,348,431 bp
  • A to G, chromosome 11 at 106,684,281 bp
  • T to C, chromosome 11 at 120,793,104 bp
  • T to C, chromosome 12 at 16,965,091 bp
  • A to G, chromosome 12 at 86,244,315 bp
  • G to A, chromosome 13 at 3,574,359 bp
  • T to C, chromosome 14 at 118,911,368 bp
  • T to C, chromosome 15 at 89,145,450 bp
  • A to G, chromosome 17 at 46,242,247 bp
  • G to A, chromosome 17 at 56,876,348 bp
  • C to A, chromosome 19 at 61,227,320 bp
  • A to T, chromosome X at 77,771,275 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3149 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040601-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.