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Strain Name:
C57BL/6J-MtgxR3154Btlr/Mmmh
Stock Number:
040605-MU
Citation ID:
RRID:MMRRC_040605-MU
Other Names:
R3154 (G1), C57BL/6J-MtgxR3154Btlr
Major Collection:

Strain Information

Chrna3
Name: cholinergic receptor, nicotinic, alpha polypeptide 3
Synonyms: (a)3, alpha 3, neuronal nicotinic acetylcholine receptor, alpha 3 subunit, Acra-3, Acra3, A730007P14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110834
VEGA: 9
HGNC: HGNC:1957
Homologene: 591
Penk
Name: preproenkephalin
Synonyms: ENK, Penk, PPA, Penk1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18619
HGNC: HGNC:8831
Homologene: 4528
Gsc2
Name: goosecoid homebox 2
Synonyms: 4930568H22Rik, Gscl
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 195333
HGNC: HGNC:4613
Homologene: 3884
Htr2a
Name: 5-hydroxytryptamine (serotonin) receptor 2A
Synonyms: 5-HT2A receptor, Htr-2, Htr2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 15558
VEGA: 14
HGNC: HGNC:5293
Homologene: 68073
Mthfr
Name: methylenetetrahydrofolate reductase
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17769
HGNC: HGNC:7436
Homologene: 4349
Dgat1
Name: diacylglycerol O-acyltransferase 1
Synonyms: D15Ertd23e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 13350
HGNC: HGNC:2843
Homologene: 7688
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 36,521,222 bp
  • A to T, chromosome 1 at 71,593,083 bp
  • T to A, chromosome 1 at 75,401,542 bp
  • C to A, chromosome 1 at 88,205,051 bp
  • T to C, chromosome 1 at 134,741,861 bp
  • T to C, chromosome 2 at 28,579,536 bp
  • A to T, chromosome 2 at 65,107,050 bp
  • C to T, chromosome 2 at 65,330,335 bp
  • A to G, chromosome 2 at 73,437,490 bp
  • A to C, chromosome 2 at 152,440,824 bp
  • G to A, chromosome 3 at 130,549,168 bp
  • T to C, chromosome 4 at 4,134,152 bp
  • A to G, chromosome 4 at 63,970,449 bp
  • T to A, chromosome 4 at 139,965,713 bp
  • T to G, chromosome 4 at 148,051,604 bp
  • T to A, chromosome 5 at 16,246,653 bp
  • A to G, chromosome 5 at 148,303,273 bp
  • C to T, chromosome 6 at 49,245,512 bp
  • T to C, chromosome 6 at 50,251,363 bp
  • A to G, chromosome 6 at 113,593,269 bp
  • A to T, chromosome 6 at 131,310,012 bp
  • T to C, chromosome 7 at 9,094,690 bp
  • C to A, chromosome 7 at 18,426,423 bp
  • T to A, chromosome 7 at 141,792,736 bp
  • G to T, chromosome 8 at 34,856,079 bp
  • T to C, chromosome 8 at 95,744,278 bp
  • T to A, chromosome 8 at 125,135,304 bp
  • T to C, chromosome 9 at 21,281,894 bp
  • G to A, chromosome 9 at 21,589,901 bp
  • A to G, chromosome 9 at 21,980,774 bp
  • T to C, chromosome 9 at 54,885,946 bp
  • C to T, chromosome 9 at 55,016,050 bp
  • G to A, chromosome 9 at 62,282,094 bp
  • A to G, chromosome 9 at 109,999,792 bp
  • T to C, chromosome 10 at 14,523,297 bp
  • T to C, chromosome 10 at 52,050,981 bp
  • A to T, chromosome 10 at 79,761,631 bp
  • T to C, chromosome 11 at 5,023,135 bp
  • T to A, chromosome 11 at 60,479,360 bp
  • A to T, chromosome 11 at 120,807,939 bp
  • T to C, chromosome 13 at 24,943,620 bp
  • G to T, chromosome 13 at 24,956,163 bp
  • A to C, chromosome 13 at 25,102,357 bp
  • T to C, chromosome 13 at 30,947,253 bp
  • A to G, chromosome 13 at 67,138,655 bp
  • A to T, chromosome 13 at 100,049,814 bp
  • A to T, chromosome 14 at 55,863,587 bp
  • T to C, chromosome 14 at 74,705,822 bp
  • T to C, chromosome 14 at 119,868,977 bp
  • G to A, chromosome 15 at 76,502,521 bp
  • T to C, chromosome 15 at 82,617,566 bp
  • T to G, chromosome 15 at 97,384,542 bp
  • T to A, chromosome 15 at 98,118,209 bp
  • G to A, chromosome 16 at 17,914,500 bp
  • A to G, chromosome 16 at 96,176,280 bp
  • T to A, chromosome 17 at 24,328,746 bp
  • T to A, chromosome 17 at 33,960,511 bp
  • T to A, chromosome 17 at 36,871,688 bp
  • T to A, chromosome 17 at 40,208,243 bp
  • T to C, chromosome 17 at 45,454,082 bp
  • G to A, chromosome X at 58,031,478 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3154 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040605-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.