Strain Name:
C57BL/6J-MtgxR3177Btlr/Mmmh
Stock Number:
040615-MU
Citation ID:
RRID:MMRRC_040615-MU
Other Names:
R3177 (G1), C57BL/6J-MtgxR3177Btlr
Major Collection:

Gene Information

Vps13c
Name: vacuolar protein sorting 13C
Synonyms: C230055H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320528
VEGA: 9
Homologene: 41188
Gpatch2l
Name: G patch domain containing 2 like
Synonyms: 1700020O03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 70373
VEGA: 12
Homologene: 9942
Rell1
Name: RELT-like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100532
Homologene: 27084
Pwp1
Name: PWP1 homolog, endonuclein
Synonyms: 2310058A11Rik, 2610205J09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 103136
Homologene: 5129
Hpgd
Name: hydroxyprostaglandin dehydrogenase 15 (NAD)
Synonyms: 15-PGDH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 15446
HGNC: HGNC:5154
Homologene: 68095
Btnl9
Name: butyrophilin-like 9
Synonyms: D330012D11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237754
Homologene: 68540
Cyp4f18
Name: cytochrome P450, family 4, subfamily f, polypeptide 18
Synonyms: 1810054N16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72054
HGNC: HGNC:2645
Homologene: 128623
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: Shn-2, Schnurri-2, MIBP1, Gm20114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Itgad
Name: integrin, alpha D
Synonyms: Cd11d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381924
HGNC: HGNC:6146
Homologene: 56919
Flg2
Name: filaggrin family member 2
Synonyms: EG229574
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229574
Homologene: 134146
Padi6
Name: peptidyl arginine deiminase, type VI
Synonyms: Padi5, Pad6, ePAD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242726
Homologene: 17695
Parp9
Name: poly (ADP-ribose) polymerase family, member 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 80285
Homologene: 12720
Ano9
Name: anoctamin 9
Synonyms: Tp53i5, Tmem16j, Trp53i5, 5430425C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71345
Homologene: 67083
Ccdc178
Name: coiled coil domain containing 178
Synonyms: 4921528I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 70950
VEGA: 18
Homologene: 12373
Gm5581
Name: predicted gene 5581
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Adarb2
Name: adenosine deaminase, RNA-specific, B2
Synonyms: RED2, Adar3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 94191
HGNC: HGNC:227
Homologene: 10276
Rxrg
Name: retinoid X receptor gamma
Synonyms: Nr2b3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20183
Homologene: 21373
Zswim9
Name: zinc finger SWIM-type containing 9
Synonyms: 6330408A02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 321008
Homologene: 52386
Fbxw2
Name: F-box and WD-40 domain protein 2
Synonyms: MD6, Fwd2, FBW2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 30050
Homologene: 8131
Hacd4
Name: 3-hydroxyacyl-CoA dehydratase 4
Synonyms: 4933428I03Rik, Ptplad2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 66775
Homologene: 12033
Hao2
Name: hydroxyacid oxidase 2
Synonyms: Hao3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 56185
HGNC: HGNC:4810
Homologene: 97412
Gm5592
Name: predicted gene 5592
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434172
Homologene: 45597
Olfr1231
Name: olfactory receptor 1231
Synonyms: GA_x6K02T2Q125-50748233-50747292, MOR235-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258446
Homologene: 27168
Mtmr11
Name: myotubularin related protein 11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 194126
Homologene: 82382
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 167,635,700 bp
  • T to C, chromosome 2 at 34,822,750 bp
  • C to T, chromosome 2 at 89,303,218 bp
  • A to T, chromosome 3 at 93,214,888 bp
  • T to C, chromosome 3 at 96,167,891 bp
  • T to C, chromosome 3 at 98,880,328 bp
  • T to C, chromosome 4 at 88,437,510 bp
  • A to G, chromosome 4 at 140,735,389 bp
  • A to G, chromosome 5 at 63,926,987 bp
  • C to G, chromosome 6 at 131,166,965 bp
  • T to C, chromosome 7 at 13,277,270 bp
  • A to G, chromosome 7 at 41,288,380 bp
  • C to A, chromosome 7 at 128,190,981 bp
  • T to A, chromosome 7 at 141,104,124 bp
  • C to A, chromosome 8 at 56,298,413 bp
  • T to C, chromosome 8 at 71,993,200 bp
  • T to C, chromosome 9 at 67,948,108 bp
  • C to A, chromosome 10 at 14,128,969 bp
  • C to T, chromosome 10 at 85,882,079 bp
  • T to C, chromosome 11 at 49,169,676 bp
  • A to G, chromosome 12 at 86,244,315 bp
  • A to G, chromosome 13 at 8,752,627 bp
  • T to C, chromosome 16 at 35,948,208 bp
  • G to T, chromosome 18 at 22,067,652 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3177 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040615-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.