Strain Name:
C57BL/6J-MtgxR3195Btlr/Mmmh
Stock Number:
040616-MU
Citation ID:
RRID:MMRRC_040616-MU
Other Names:
R3195 (G1), C57BL/6J-MtgxR3195Btlr
Major Collection:

Gene Information

Calb2
Name: calbindin 2
Synonyms: CR, calretinin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 12308
HGNC: HGNC:1435
Homologene: 1318
Dmtf1
Name: cyclin D binding myb-like transcription factor 1
Synonyms: Dmp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 23857
Homologene: 8017
Focad
Name: focadhesin
Synonyms: BC057079
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230393
Homologene: 9842
Frem1
Name: Fras1 related extracellular matrix protein 1
Synonyms: eyem02Jus, heb, eyes2, crf11, QBRICK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 329872
Homologene: 27049
Spg11
Name: SPG11, spatacsin vesicle trafficking associated
Synonyms: spastic paraplegia 11, 6030465E24Rik, C530005A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 214585
Homologene: 41614
Gapdh
Name: glyceraldehyde-3-phosphate dehydrogenase
Synonyms: Gapd, Gapd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 14433
HGNC: HGNC:4141
Homologene: 107053
Lpin1
Name: lipin 1
Synonyms: Lipin1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 14245
VEGA: 12
Homologene: 9266
Gab2
Name: growth factor receptor bound protein 2-associated protein 2
Synonyms: D130058I17Rik, p97
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 14389
Homologene: 69067
Csgalnact1
Name: chondroitin sulfate N-acetylgalactosaminyltransferase 1
Synonyms: 4732435N03Rik, CSGalNAcT-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234356
Homologene: 23099
Hspg2
Name: perlecan (heparan sulfate proteoglycan 2)
Synonyms: Pcn, Plc, perlecan, per
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 15530
HGNC: HGNC:5273
Homologene: 68473
Vamp4
Name: vesicle-associated membrane protein 4
Synonyms: D1Ertd147e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 53330
Homologene: 37847
Pes1
Name: pescadillo ribosomal biogenesis factor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 64934
HGNC: HGNC:8848
Homologene: 5984
Kirrel
Name: kirre like nephrin family adhesion molecule 1
Synonyms: Neph1, Kirrel1, 6720469N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 170643
Homologene: 10089
Usp42
Name: ubiquitin specific peptidase 42
Synonyms: 2410140K03Rik, D5Ertd591e, 3110031A07Rik, A630018G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 76800
Homologene: 35425
L1td1
Name: LINE-1 type transposase domain containing 1
Synonyms: ECAT11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 381591
Homologene: 135709
Pde4b
Name: phosphodiesterase 4B, cAMP specific
Synonyms: Dpde4, dunce
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 18578
HGNC: HGNC:8781
Homologene: 1953
Akap6
Name: A kinase (PRKA) anchor protein 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238161
VEGA: 12
HGNC: HGNC:376
Homologene: 3157
Reln
Name: reelin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Sh3rf1
Name: SH3 domain containing ring finger 1
Synonyms: Posh, 2200003J05Rik, Sh3md2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 59009
Homologene: 10988
Trpm1
Name: transient receptor potential cation channel, subfamily M, member 1
Synonyms: Mlsn1, LTRPC1, melastatin, 4732499L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 17364
HGNC: HGNC:7146
Homologene: 19940
Olfr395
Name: olfactory receptor 395
Synonyms: MOR135-10, GA_x6K02T2P1NL-4062605-4061667
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 259007
Fbxw26
Name: F-box and WD-40 domain protein 26
Synonyms: Gm5163
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 382109
Homologene: 110776
Slc13a4
Name: solute carrier family 13 (sodium/sulfate symporters), member 4
Synonyms: SUT1, 9630060C05Rik, SUT-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 243755
Homologene: 69125
Myo1h
Name: myosin 1H
Synonyms: 4631401O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231646
Homologene: 82639
Smarca2
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Synonyms: brm, Snf2l2, 2610209L14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 67155
Homologene: 2308
Paqr8
Name: progestin and adipoQ receptor family member VIII
Synonyms: 1700019B16Rik, 3110001D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 74229
Homologene: 32702
Iqub
Name: IQ motif and ubiquitin domain containing
Synonyms: 4932408B21Rik, Trs4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 214704
Homologene: 44790
Cdh15
Name: cadherin 15
Synonyms: Mcad, Cdh14, M cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 12555
HGNC: HGNC:1754
Homologene: 3622
9130401M01Rik
Name: RIKEN cDNA 9130401M01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 75758
Homologene: 12622
Pde8b
Name: phosphodiesterase 8B
Synonyms: C030047E14Rik, B230331L10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 218461
HGNC: HGNC:8794
Homologene: 2758
Nfkbiz
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta
Synonyms: Mail
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 80859
Homologene: 12734
Skint9
Name: selection and upkeep of intraepithelial T cells 9
Synonyms: A030013N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 329918
Homologene: 136292
Lilra5
Name: leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5
Synonyms: Gm4878
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 232801
Homologene: 83297
Acnat1
Name: acyl-coenzyme A amino acid N-acyltransferase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230161
Homologene: 28287
Zfp957
Name: zinc finger protein 957
Synonyms: AU017455
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 105590
Olfr95
Name: olfactory receptor 95
Synonyms: MOR263-6, GA_x6K02T2PSCP-1651760-1650822
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 258506
HGNC: HGNC:8165
Homologene: 71990
Apmap
Name: adipocyte plasma membrane associated protein
Synonyms: 2310001A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 71881
Homologene: 41380
CT573034.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
Mmp7
Name: matrix metallopeptidase 7
Synonyms: MAT, matrilysin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 17393
HGNC: HGNC:7174
Homologene: 37619
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 20,935,033 bp
  • A to G, chromosome 1 at 162,574,427 bp
  • A to G, chromosome 2 at 122,083,398 bp
  • T to A, chromosome 2 at 150,584,201 bp
  • A to T, chromosome 2 at 150,584,202 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • G to C, chromosome 4 at 49,447,457 bp
  • A to G, chromosome 4 at 83,014,114 bp
  • A to G, chromosome 4 at 88,407,351 bp
  • A to G, chromosome 4 at 98,737,518 bp
  • G to A, chromosome 4 at 102,599,643 bp
  • T to G, chromosome 4 at 112,390,951 bp
  • C to T, chromosome 4 at 137,511,380 bp
  • A to G, chromosome 5 at 9,132,454 bp
  • A to T, chromosome 5 at 22,040,420 bp
  • G to A, chromosome 5 at 114,328,740 bp
  • A to G, chromosome 5 at 143,717,199 bp
  • G to C, chromosome 6 at 24,462,037 bp
  • C to T, chromosome 6 at 35,268,926 bp
  • T to C, chromosome 6 at 125,162,620 bp
  • G to A, chromosome 7 at 4,238,757 bp
  • T to A, chromosome 7 at 64,199,313 bp
  • A to G, chromosome 7 at 97,272,029 bp
  • C to T, chromosome 8 at 61,226,287 bp
  • TGG to TG, chromosome 8 at 68,461,085 bp
  • C to T, chromosome 8 at 110,157,003 bp
  • G to A, chromosome 8 at 122,856,635 bp
  • C to A, chromosome 9 at 7,692,218 bp
  • AGGG to AGGGGGGG, chromosome 9 at 109,732,488 bp
  • T to C, chromosome 11 at 3,975,736 bp
  • T to C, chromosome 11 at 73,906,658 bp
  • A to G, chromosome 12 at 16,565,583 bp
  • T to A, chromosome 12 at 53,072,457 bp
  • A to T, chromosome 13 at 62,370,054 bp
  • A to G, chromosome 13 at 95,222,460 bp
  • A to G, chromosome 14 at 79,212,892 bp
  • A to T, chromosome 15 at 58,028,736 bp
  • A to G, chromosome 16 at 55,819,628 bp
  • A to G, chromosome 17 at 37,211,536 bp
  • A to G, chromosome 19 at 26,683,822 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3195 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040616-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.