Strain Name:
C57BL/6J-MtgxR3236Btlr/Mmmh
Stock Number:
040618-MU
Citation ID:
RRID:MMRRC_040618-MU
Other Names:
R3236 (G1), C57BL/6J-MtgxR3236Btlr
Major Collection:

Strain Information

Notch3
Name: notch 3
Synonyms: hpbk, N3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376
Slc1a3
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3
Synonyms: MGluT1, Eaat1, B430115D02Rik, GLAST, Gmt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20512
Homologene: 20882
Ptf1a
Name: pancreas specific transcription factor, 1a
Synonyms: PTF1-p48, bHLHa29
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19213
Homologene: 10304
Ndrg3
Name: N-myc downstream regulated gene 3
Synonyms: Ndr3, 4833415O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 29812
Homologene: 40903
Bcar3
Name: breast cancer anti-estrogen resistance 3
Synonyms: AND-34
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 29815
HGNC: HGNC:973
Homologene: 31181
Lrig3
Name: leucine-rich repeats and immunoglobulin-like domains 3
Synonyms: 9030421L11Rik, 9130004I02Rik, 9430095K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 320398
VEGA: 10
Homologene: 62452
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Col6a1
Name: collagen, type VI, alpha 1
Synonyms: Col6a-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12833
HGNC: HGNC:2211
Homologene: 1391
Armh3
Name: armadillo-like helical domain containing 3
Synonyms: 9130011E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 71617
VEGA: 19
Homologene: 15843
Phf20l1
Name: PHD finger protein 20-like 1
Synonyms: CGI-72, E130113K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239510
VEGA: 15
Homologene: 9341
Sf3b3
Name: splicing factor 3b, subunit 3
Synonyms: 5730409A01Rik, 1810061H24Rik, SAP130, RSE1, D8Ertd633e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 101943
Homologene: 6579
Snrnp200
Name: small nuclear ribonucleoprotein 200 (U5)
Synonyms: U5-200KD, HELIC2, A330064G03Rik, Ascc3l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320632
Homologene: 5859
Supt20
Name: SPT20 SAGA complex component
Synonyms: p38 interacting protein, p38IP, D3Ertd300e, Fam48a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 56790
Homologene: 134155
Setdb1
Name: SET domain, bifurcated 1
Synonyms: ESET, KMT1E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 84505
Homologene: 32157
Zgrf1
Name: zinc finger, GRF-type containing 1
Synonyms: 4930422G04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71643
Homologene: 34708
Rasgrp1
Name: RAS guanyl releasing protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19419
HGNC: HGNC:9878
Homologene: 4195
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: 1810042B05Rik, Coh1, C330002D13Rik, 2310042E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Scaf4
Name: SR-related CTD-associated factor 4
Synonyms: Sra4, Sfrs15, Srsf15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224432
VEGA: 16
Homologene: 16227
Slf2
Name: SMC5-SMC6 complex localization factor 2
Synonyms: 6030443O07Rik, Fam178a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226151
VEGA: 19
Homologene: 23077
Hk1
Name: hexokinase 1
Synonyms: Hk-1, mHk1-s, Hk1-s
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15275
HGNC: HGNC:4922
Homologene: 100530
Pon2
Name: paraoxonase 2
Synonyms: 6330405I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330260
HGNC: HGNC:9205
Homologene: 385
Usp39
Name: ubiquitin specific peptidase 39
Synonyms: D6Wsu157e, CGI-21, SAD1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 28035
Homologene: 13183
Morc2a
Name: microrchidia 2A
Synonyms: 8430403M08Rik, Zcwcc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74522
Homologene: 8966
Memo1
Name: mediator of cell motility 1
Synonyms: D930048L02Rik, 0610016J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 76890
VEGA: 17
Homologene: 6272
2700049A03Rik
Name: RIKEN cDNA 2700049A03 gene
Synonyms: talpid3, Ta3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 76967
Homologene: 8839
Ccdc88a
Name: coiled coil domain containing 88A
Synonyms: C330012F17Rik, D130005J21Rik, C130096N06Rik, GIV, HkRP1, Girdin, A430106J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 108686
Homologene: 41180
Kdm6b
Name: KDM1 lysine (K)-specific demethylase 6B
Synonyms: Jmjd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216850
Homologene: 18945
Szt2
Name: SZT2 subunit of KICSTOR complex
Synonyms: seaizure threshold 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230676
Homologene: 49413
Plxna2
Name: plexin A2
Synonyms: OCT, Plxn2, 2810428A13Rik, PlexA2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18845
HGNC: HGNC:9100
Homologene: 56427
Cd34
Name: CD34 antigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12490
HGNC: HGNC:1662
Homologene: 1343
Pdgfra
Name: platelet derived growth factor receptor, alpha polypeptide
Synonyms: CD140a, Pdgfr-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18595
HGNC: HGNC:8803
Homologene: 31361
Tmem167
Name: transmembrane protein 167
Synonyms: 5730424F14Rik, 0610041E09Rik, Gm10085
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66074
Homologene: 41626
Aff2
Name: AF4/FMR2 family, member 2
Synonyms: Ox19, Oxh, Fmr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 14266
HGNC: HGNC:3776
Homologene: 1532
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Fyb
Name: FYN binding protein
Synonyms: FYB-120/130, ADAP, B630013F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 23880
HGNC: HGNC:4036
Homologene: 22664
Col5a3
Name: collagen, type V, alpha 3
Synonyms: Pro-alpha3(V)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 53867
Homologene: 9253
Dnah17
Name: dynein, axonemal, heavy chain 17
Synonyms: LOC382552, 2810003K23Rik, Dnahcl1, Dnahc17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69926
HGNC: HGNC:2946
Homologene: 72102
Dnah9
Name: dynein, axonemal, heavy chain 9
Synonyms: D11Ertd686e, Dnahc9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237806
HGNC: HGNC:2953
Homologene: 20357
Treml4
Name: triggering receptor expressed on myeloid cells-like 4
Synonyms: 5031403H21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224840
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Map6
Name: microtubule-associated protein 6
Synonyms: F-STOP, STOP, 2810411E12Rik, Mtap6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17760
HGNC: HGNC:6868
Homologene: 7850
Agbl3
Name: ATP/GTP binding protein-like 3
Synonyms: 2900053G10Rik, 6530406M24Rik, 4930431N21Rik, Ccp3, Ccp3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 76223
Homologene: 35330
Or14j10
Name: olfactory receptor family 14 subfamily J member 10
Synonyms: MOR218-2, GA_x6K02T2PSCP-2084102-2083137, Olfr116
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258625
Lipn
Name: lipase, family member N
Synonyms: 2210418G03Rik, Lipl4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 70166
Homologene: 66969
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435626
Homologene: 52359
Krt76
Name: keratin 76
Synonyms: 2310001L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 77055
Homologene: 22931
Eml6
Name: echinoderm microtubule associated protein like 6
Synonyms: 2900083P10Rik, C230094A16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237711
Homologene: 104282
Spata22
Name: spermatogenesis associated 22
Synonyms: LOC380709
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380709
Homologene: 13076
Stat6
Name: signal transducer and activator of transcription 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 20852
Homologene: 2373
Thsd7b
Name: thrombospondin, type I, domain containing 7B
Synonyms: 1700074E13Rik, D130067I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210417
Homologene: 18180
Thsd4
Name: thrombospondin, type I, domain containing 4
Synonyms: B230114P05Rik, ADAMTSL6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 207596
Homologene: 49796
Vmn2r112
Name: vomeronasal 2, receptor 112
Synonyms: EG628185
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 628185
Homologene: 86604
AC120787.1
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Stard6
Name: StAR-related lipid transfer (START) domain containing 6
Synonyms: 4833424I06Rik, 4933429L05Rik, 1700011K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 170461
Homologene: 12578
Vmn2r24
Name: vomeronasal 2, receptor 24
Synonyms: EG243628
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243628
Homologene: 135915
Ecm1
Name: extracellular matrix protein 1
Synonyms: p85
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 13601
HGNC: HGNC:3153
Homologene: 3260
Cyp3a25
Name: cytochrome P450, family 3, subfamily a, polypeptide 25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 56388
Homologene: 135775
5730455P16Rik
Name: RIKEN cDNA 5730455P16 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70591
Homologene: 11177
Fbh1
Name: F-box DNA helicase 1
Synonyms: Fbx18, Fbxo18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 50755
Homologene: 9272
Or4p7
Name: olfactory receptor family 4 subfamily P member 7
Synonyms: GA_x6K02T2Q125-49870417-49871388, MOR225-6P, Olfr1178
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258203
Homologene: 73979
H2-Q6
Name: histocompatibility 2, Q region locus 6
Synonyms: Qa6, Qa-6, H-2Q6, 0610037M15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 110557
Homologene: 128352
Rbfox1
Name: RNA binding protein, fox-1 homolog (C. elegans) 1
Synonyms: A2bp, HRNBP1, FOX1, A2bp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 268859
VEGA: 16
Homologene: 69339
Prl7b1
Name: prolactin family 7, subfamily b, member 1
Synonyms: PLP-N, 1600014J19Rik, Prlpn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 75596
HGNC: HGNC:9445
Homologene: 17809
Speer4e
Name: spermatogenesis associated glutamate (E)-rich protein 4e
Synonyms: SPEER-4E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 624245
Homologene: 69402
N4bp3
Name: NEDD4 binding protein 3
Synonyms: C330016O10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 212706
Homologene: 17124
Serhl
Name: serine hydrolase-like
Synonyms: 1110019M09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 68607
Homologene: 65196
Rrh
Name: retinal pigment epithelium derived rhodopsin homolog
Synonyms: Peropsin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20132
Homologene: 55977
Timm8a2
Name: translocase of inner mitochondrial membrane 8A2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 223262
Homologene: 86868
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 74,147,663 bp
  • C to T, chromosome 1 at 130,218,118 bp
  • C to T, chromosome 1 at 194,749,317 bp
  • A to G, chromosome 1 at 194,959,245 bp
  • T to C, chromosome 2 at 11,769,826 bp
  • T to C, chromosome 2 at 19,445,907 bp
  • T to C, chromosome 2 at 76,899,409 bp
  • T to C, chromosome 2 at 88,391,406 bp
  • A to G, chromosome 2 at 117,291,812 bp
  • T to A, chromosome 2 at 127,221,882 bp
  • T to A, chromosome 2 at 156,944,037 bp
  • T to C, chromosome 3 at 54,709,080 bp
  • A to G, chromosome 3 at 95,338,754 bp
  • G to T, chromosome 3 at 95,734,846 bp
  • C to A, chromosome 3 at 122,524,996 bp
  • A to G, chromosome 3 at 127,613,375 bp
  • T to C, chromosome 3 at 129,811,711 bp
  • A to C, chromosome 4 at 118,383,034 bp
  • T to A, chromosome 5 at 14,934,925 bp
  • T to C, chromosome 5 at 75,167,936 bp
  • T to C, chromosome 5 at 146,003,128 bp
  • A to T, chromosome 6 at 5,266,986 bp
  • A to G, chromosome 6 at 34,823,087 bp
  • G to A, chromosome 6 at 72,338,389 bp
  • C to T, chromosome 6 at 123,779,025 bp
  • T to A, chromosome 7 at 99,336,824 bp
  • A to G, chromosome 8 at 110,812,020 bp
  • G to T, chromosome 9 at 20,807,653 bp
  • T to A, chromosome 9 at 53,479,748 bp
  • C to A, chromosome 9 at 60,394,387 bp
  • T to C, chromosome 10 at 62,296,019 bp
  • G to A, chromosome 10 at 76,711,320 bp
  • G to A, chromosome 10 at 125,997,187 bp
  • G to A, chromosome 10 at 127,652,222 bp
  • T to C, chromosome 11 at 3,683,612 bp
  • A to G, chromosome 11 at 29,447,995 bp
  • A to G, chromosome 11 at 29,831,097 bp
  • A to T, chromosome 11 at 51,645,934 bp
  • T to C, chromosome 11 at 59,131,646 bp
  • G to A, chromosome 11 at 65,954,989 bp
  • A to G, chromosome 11 at 69,406,366 bp
  • T to A, chromosome 11 at 73,345,887 bp
  • G to A, chromosome 11 at 80,368,170 bp
  • T to C, chromosome 11 at 118,094,854 bp
  • G to T, chromosome 12 at 71,164,546 bp
  • A to T, chromosome 12 at 71,164,547 bp
  • T to A, chromosome 13 at 27,602,900 bp
  • C to T, chromosome 13 at 90,104,380 bp
  • A to G, chromosome 14 at 122,035,015 bp
  • A to C, chromosome 15 at 6,630,116 bp
  • T to C, chromosome 15 at 8,639,123 bp
  • G to A, chromosome 15 at 35,910,304 bp
  • T to C, chromosome 15 at 66,609,514 bp
  • C to A, chromosome 15 at 83,104,403 bp
  • A to G, chromosome 15 at 101,644,303 bp
  • A to G, chromosome 15 at 101,888,989 bp
  • G to A, chromosome 16 at 7,408,028 bp
  • T to A, chromosome 16 at 90,260,217 bp
  • T to A, chromosome 17 at 22,603,115 bp
  • T to C, chromosome 17 at 32,158,461 bp
  • C to A, chromosome 17 at 35,425,700 bp
  • T to C, chromosome 17 at 37,624,236 bp
  • T to C, chromosome 17 at 48,274,369 bp
  • A to T, chromosome 17 at 74,202,352 bp
  • T to A, chromosome 18 at 70,500,486 bp
  • A to G, chromosome 19 at 34,068,738 bp
  • A to G, chromosome 19 at 44,942,334 bp
  • A to T, chromosome 19 at 45,975,283 bp
  • T to A, chromosome X at 69,863,937 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3236 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040618-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.