Strain Name:
C57BL/6J-MtgxR3402Btlr/Mmmh
Stock Number:
040621-MU
Citation ID:
RRID:MMRRC_040621-MU
Other Names:
R3402 (G1), C57BL/6J-MtgxR3402Btlr
Major Collection:

Gene Information

Slc6a6
Name: solute carrier family 6 (neurotransmitter transporter, taurine), member 6
Synonyms: Taut
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 21366
Homologene: 2291
Dysf
Name: dysferlin
Synonyms: 2310004N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 26903
HGNC: HGNC:3097
Homologene: 20748
Kat2b
Name: K(lysine) acetyltransferase 2B
Synonyms: A930006P13Rik, Pcaf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18519
HGNC: HGNC:8638
Homologene: 20834
Ppp1r37
Name: protein phosphatase 1, regulatory subunit 37
Synonyms: Lrrc68
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232947
Homologene: 17851
Etl4
Name: enhancer trap locus 4
Synonyms: Etl-4, E330027G05Rik, 6620402G01Rik, 9430077C05Rik, Skt, Sickle tail
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 208618
Homologene: 10477
Dennd4c
Name: DENN/MADD domain containing 4C
Synonyms: 1700065A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329877
Homologene: 23057
Afdn
Name: afadin, adherens junction formation factor
Synonyms: AF6, Afadin, S-afadin, I-afadin, 5033403D15Rik, Mllt4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 17356
HGNC: HGNC:7137
Homologene: 21202
Dhx40
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 40
Synonyms: ARG147, DDX40, 2410016C14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67487
Homologene: 57000
Trp53bp2
Name: transformation related protein 53 binding protein 2
Synonyms: ASPP2, 53BP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 209456
Homologene: 3959
Fntb
Name: farnesyltransferase, CAAX box, beta
Synonyms: 2010013E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 110606
HGNC: HGNC:3785
Homologene: 1535
Zfp180
Name: zinc finger protein 180
Synonyms: D130011P11, HHZ168, 2310040I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 210135
Homologene: 8306
Stk3
Name: serine/threonine kinase 3
Synonyms: mess1, 0610042I06Rik, Mst2, MST, Ste20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 56274
Homologene: 48420
Prkcq
Name: protein kinase C, theta
Synonyms: PKC-0, Pkcq, A130035A12Rik, PKC-theta, PKC theta, PKCtheta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18761
HGNC: HGNC:9410
Homologene: 21263
Cd3e
Name: CD3 antigen, epsilon polypeptide
Synonyms: T3e, CD3, CD3epsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12501
HGNC: HGNC:1674
Homologene: 586
Slit2
Name: slit guidance ligand 2
Synonyms: Slil3, Drad-1, E130320P19Rik, E030015M03Rik, b2b1200.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20563
Homologene: 3516
Atp10a
Name: ATPase, class V, type 10A
Synonyms: pfatp, Atp10c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11982
Homologene: 56461
Myo16
Name: myosin XVI
Synonyms: C230040D10Rik, Nyap3, BM140241
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244281
Homologene: 34710
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Dnah3
Name: dynein, axonemal, heavy chain 3
Synonyms: Dnahc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381917
HGNC: HGNC:2949
Homologene: 19674
Unc80
Name: unc-80, NALCN activator
Synonyms: C230061B10Rik, C030018G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329178
Homologene: 122243
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: VLGR1, Mass1, Mgr1, Gpr98
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 110789
Homologene: 19815
Adamts3
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 3
Synonyms: 6330442E02Rik, 1100001H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330119
HGNC: HGNC:219
Homologene: 8596
Olfr1477
Name: olfactory receptor 1477
Synonyms: GA_x6K02T2RE5P-3834960-3835907, MOR202-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258691
Homologene: 110492
Tma16
Name: translation machinery associated 16
Synonyms: 1810029B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66282
Homologene: 10146
Pkn1
Name: protein kinase N1
Synonyms: Pkn, PRK1, PAK1, Stk3, Prkcl1, F730027O18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 320795
HGNC: HGNC:9405
Homologene: 48130
Tcaf3
Name: TRPM8 channel-associated factor 3
Synonyms: Eapa2, Fam115e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 403088
Homologene: 74374
Nlrp4d
Name: NLR family, pyrin domain containing 4D
Synonyms: Nalp-beta, Nalp4d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 384752
Adam22
Name: a disintegrin and metallopeptidase domain 22
Synonyms: MDC2, 2900022I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11496
HGNC: HGNC:201
Homologene: 37898
Hip1r
Name: huntingtin interacting protein 1 related
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 29816
Homologene: 78348
Cactin
Name: cactin, spliceosome C complex subunit
Synonyms: 2510012J08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70312
VEGA: 10
Homologene: 69553
Sel1l2
Name: sel-1 suppressor of lin-12-like 2 (C. elegans)
Synonyms: LOC228684
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228684
Homologene: 16802
Olfr1378
Name: olfactory receptor 1378
Synonyms: GA_x6K02T2QP88-4469162-4468215, MOR129-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258912
Homologene: 133679
Tmem74
Name: transmembrane protein 74
Synonyms: B230382K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239408
VEGA: 15
Homologene: 17684
Upk3a
Name: uroplakin 3A
Synonyms: 1110017C07Rik, Upk3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 22270
VEGA: 15
Homologene: 5066
Hspb11
Name: heat shock protein family B (small), member 11
Synonyms: 2900042B11Rik, IFT25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 72938
Homologene: 22947
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 66,510,686 bp
  • C to A, chromosome 1 at 182,455,872 bp
  • A to G, chromosome 2 at 11,283,849 bp
  • A to T, chromosome 2 at 20,781,882 bp
  • T to A, chromosome 2 at 140,241,038 bp
  • C to T, chromosome 4 at 86,774,543 bp
  • A to G, chromosome 4 at 107,273,606 bp
  • A to T, chromosome 5 at 8,086,581 bp
  • T to C, chromosome 5 at 48,283,421 bp
  • A to T, chromosome 5 at 89,701,733 bp
  • A to G, chromosome 5 at 123,996,983 bp
  • T to C, chromosome 6 at 42,593,853 bp
  • G to A, chromosome 6 at 84,186,509 bp
  • A to G, chromosome 6 at 91,726,129 bp
  • A to T, chromosome 7 at 10,362,854 bp
  • C to T, chromosome 7 at 19,532,787 bp
  • G to A, chromosome 7 at 24,105,745 bp
  • T to A, chromosome 7 at 58,774,055 bp
  • A to G, chromosome 7 at 119,967,656 bp
  • A to G, chromosome 8 at 10,384,719 bp
  • C to T, chromosome 8 at 66,484,171 bp
  • G to A, chromosome 8 at 83,670,230 bp
  • T to C, chromosome 9 at 45,000,426 bp
  • T to C, chromosome 9 at 79,643,947 bp
  • G to A, chromosome 10 at 81,325,875 bp
  • A to G, chromosome 11 at 50,969,068 bp
  • T to C, chromosome 11 at 86,798,908 bp
  • C to A, chromosome 12 at 76,887,888 bp
  • T to C, chromosome 13 at 81,543,542 bp
  • G to T, chromosome 15 at 34,944,998 bp
  • C to T, chromosome 15 at 43,867,021 bp
  • T to C, chromosome 15 at 85,018,183 bp
  • C to T, chromosome 17 at 13,883,914 bp
  • C to T, chromosome 17 at 53,665,853 bp
  • G to A, chromosome 19 at 13,502,948 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3402 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040621-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.