Strain Name:
C57BL/6J-MtgxR3408Btlr/Mmmh
Stock Number:
040626-MU
Citation ID:
RRID:MMRRC_040626-MU
Other Names:
R3408 (G1), C57BL/6J-MtgxR3408Btlr
Major Collection:

Gene Information

Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11426
Homologene: 136191
Clasrp
Name: CLK4-associating serine/arginine rich protein
Synonyms: SWAP2, Sfrs16, Srsf16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 53609
Homologene: 134306
Nr5a2
Name: nuclear receptor subfamily 5, group A, member 2
Synonyms: Ftf, LRH-1, D1Ertd308e, UF2-H3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 26424
HGNC: HGNC:7984
Homologene: 20827
Bag3
Name: BCL2-associated athanogene 3
Synonyms: Bis, Bcl-2-interacting death suppressor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 29810
HGNC: HGNC:939
Homologene: 3162
E2f7
Name: E2F transcription factor 7
Synonyms: A630014C11Rik, E2F7, D10Ertd739e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 52679
Homologene: 18685
Gpi1
Name: glucose-6-phosphate isomerase 1
Synonyms: Org, Gpi-1t, Gpi-1, Gpi-1s, Gpi-1r, Gpi1-t, Gpi1-s, Gpi1-r, MF, maturation factor, NK, AMF, autocrine motility factor, neuroleukin, NK/GPI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14751
HGNC: HGNC:4458
Homologene: 145
Med25
Name: mediator complex subunit 25
Synonyms: ESTM2, 2610529E18Rik, 2610034E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75613
Homologene: 12614
Eef2
Name: eukaryotic translation elongation factor 2
Synonyms: Ef-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 13629
VEGA: 10
HGNC: HGNC:3214
Homologene: 134867
Ipo8
Name: importin 8
Synonyms: OM-1, 6230418K12Rik, Om1, C130009K11Rik, Ranbp8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320727
HGNC: HGNC:9853
Homologene: 48430
Plekhg5
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269608
Homologene: 10768
Frem1
Name: Fras1 related extracellular matrix protein 1
Synonyms: eyem02Jus, heb, QBRICK, crf11, eyes2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329872
Homologene: 27049
Vps50
Name: VPS50 EARP/GARPII complex subunit
Synonyms: 8430415E05Rik, 1700034M03Rik, Ccdc132
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 73288
Homologene: 11498
Ap1ar
Name: adaptor-related protein complex 1 associated regulatory protein
Synonyms: Gadkin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 211556
Homologene: 10236
Rfx2
Name: regulatory factor X, 2 (influences HLA class II expression)
Synonyms: 5430432H19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19725
VEGA: 17
HGNC: HGNC:9983
Homologene: 30980
Adam17
Name: a disintegrin and metallopeptidase domain 17
Synonyms: Tace, CD156b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 11491
HGNC: HGNC:195
Homologene: 2395
Dync2i2
Name: dynein 2 intermediate chain 2
Synonyms: 3200002I06Rik, Wdr34
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71820
Homologene: 14156
Cep170b
Name: centrosomal protein 170B
Synonyms: AW555464
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217882
VEGA: 12
Homologene: 46165
Mideas
Name: mitotic deacetylase associated SANT domain protein
Synonyms: 9430029N19Rik, C130039O16Rik, Elmsan1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238317
Homologene: 19330
Myo7a
Name: myosin VIIA
Synonyms: Myo7, USH1B, nmf371, polka, Hdb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17921
HGNC: HGNC:7606
Homologene: 219
Kcnu1
Name: potassium channel, subfamily U, member 1
Synonyms: Slo3, Kcnma3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 16532
Homologene: 7392
Or9s23
Name: olfactory receptor family 9 subfamily S member 23
Synonyms: GA_x6K02T2R7CC-81180849-81179878, MOR208-1, Olfr1413
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 259039
Homologene: 128165
Nalcn
Name: sodium leak channel, non-selective
Synonyms: A530023G15Rik, Vgcnl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 338370
VEGA: 14
Homologene: 21832
Ephb3
Name: Eph receptor B3
Synonyms: Sek4, MDK5, HEK2, Etk2, Tyro6, Cek10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 13845
HGNC: HGNC:3394
Homologene: 20938
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: ENSMUSG00000044873, OTTMUSG00000020804, Heatr7b1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Aox2
Name: aldehyde oxidase 2
Synonyms: Aox3l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 213043
Homologene: 84622
Vmn2r80
Name: vomeronasal 2, receptor 80
Synonyms: EG624765
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 624765
Homologene: 83483
Ces2b
Name: carboxyesterase 2B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234669
HGNC: HGNC:1864
Homologene: 135851
Slco1b2
Name: solute carrier organic anion transporter family, member 1b2
Synonyms: mlst-1, Slc21a6, Slc21a10, Oatp1b2, 7330442B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 28253
Homologene: 75119
Or51q1c
Name: olfactory receptor family 51 subfamily Q member 1C
Synonyms: GA_x6K02T2PBJ9-6737723-6738670, MOR5-1, Olfr638
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259124
Homologene: 133592
Sh3bp4
Name: SH3-domain binding protein 4
Synonyms: BOG25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98402
Homologene: 8726
Cttnbp2
Name: cortactin binding protein 2
Synonyms: ORF4, Cortbp2, 9130022E09Rik, 4732477G22Rik, 3010022N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 30785
Homologene: 14125
Vmn1r60
Name: vomeronasal 1 receptor 60
Synonyms: Gm7184
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 636697
Homologene: 41799
Ncan
Name: neurocan
Synonyms: Tgfbit, Cspg3, Cspg3-rs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 13004
HGNC: HGNC:2465
Homologene: 3229
Cacnb3
Name: calcium channel, voltage-dependent, beta 3 subunit
Synonyms: Cchb3, Beta3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 12297
VEGA: 15
HGNC: HGNC:1403
Homologene: 20187
Or4g16
Name: olfactory receptor family 4 subfamily G member 16
Synonyms: GA_x6K02T2Q125-72357646-72358581, MOR245-12, Olfr1279
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258388
Homologene: 121520
Or10ag60
Name: olfactory receptor family 10 subfamily AG member 60
Synonyms: GA_x6K02T2Q125-49112575-49113519, MOR264-4, Olfr1130
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258835
Homologene: 133705
Piwil4
Name: piwi-like RNA-mediated gene silencing 4
Synonyms: 9230101H05Rik, Miwi2, MIWI2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 330890
Homologene: 45122
Nuak2
Name: NUAK family, SNF1-like kinase, 2
Synonyms: 1200013B22Rik, Snark
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74137
Homologene: 12539
Mfsd1
Name: major facilitator superfamily domain containing 1
Synonyms: 1200003O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66868
Homologene: 11228
Tex37
Name: testis expressed 37
Synonyms: 1700011F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74221
Homologene: 17643
Tmem150b
Name: transmembrane protein 150B
Synonyms: Tmem224
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 330460
Homologene: 19603
Adgrg4
Name: adhesion G protein-coupled receptor G4
Synonyms: LOC236798, PGR17, Gpr112
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 236798
Homologene: 72131
6430550D23Rik
Name: RIKEN cDNA 6430550D23 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320095
Homologene: 122133
Ilk
Name: integrin linked kinase
Synonyms: ESTM24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16202
HGNC: HGNC:6040
Homologene: 3318
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 58,343,668 bp
  • GCCC to GC, chromosome 1 at 88,232,257 bp
  • G to T, chromosome 1 at 89,145,047 bp
  • A to G, chromosome 1 at 92,573,953 bp
  • C to T, chromosome 1 at 132,329,943 bp
  • C to T, chromosome 1 at 136,940,498 bp
  • T to C, chromosome 2 at 30,034,753 bp
  • A to T, chromosome 2 at 87,607,876 bp
  • A to T, chromosome 2 at 111,306,505 bp
  • C to T, chromosome 2 at 156,003,920 bp
  • T to A, chromosome 3 at 67,596,713 bp
  • A to T, chromosome 3 at 127,823,259 bp
  • A to T, chromosome 4 at 83,011,986 bp
  • T to C, chromosome 4 at 123,381,781 bp
  • A to G, chromosome 4 at 152,108,292 bp
  • A to T, chromosome 6 at 3,600,212 bp
  • T to C, chromosome 6 at 18,389,242 bp
  • C to T, chromosome 6 at 70,915,706 bp
  • A to T, chromosome 6 at 141,676,256 bp
  • T to C, chromosome 6 at 148,821,709 bp
  • G to T, chromosome 7 at 4,724,340 bp
  • A to T, chromosome 7 at 5,545,149 bp
  • C to A, chromosome 7 at 19,585,240 bp
  • A to G, chromosome 7 at 34,202,679 bp
  • G to A, chromosome 7 at 44,884,759 bp
  • A to T, chromosome 7 at 98,081,087 bp
  • G to T, chromosome 7 at 104,003,343 bp
  • A to T, chromosome 7 at 105,740,974 bp
  • AAAGG to AAAGGAAGG, chromosome 7 at 128,545,769 bp
  • A to G, chromosome 8 at 25,851,958 bp
  • G to A, chromosome 8 at 70,112,151 bp
  • T to C, chromosome 8 at 104,836,469 bp
  • T to C, chromosome 9 at 14,725,963 bp
  • C to T, chromosome 10 at 79,168,393 bp
  • GCCC to GCCCC, chromosome 10 at 81,178,767 bp
  • C to T, chromosome 10 at 110,784,717 bp
  • T to A, chromosome 12 at 21,329,118 bp
  • C to T, chromosome 12 at 84,156,471 bp
  • T to C, chromosome 12 at 112,743,118 bp
  • T to A, chromosome 14 at 123,596,617 bp
  • T to C, chromosome 15 at 98,641,187 bp
  • T to A, chromosome 16 at 21,219,504 bp
  • G to T, chromosome 17 at 56,803,526 bp
  • A to T, chromosome X at 56,968,127 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3408 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040626-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.