Strain Name:
C57BL/6J-MtgxR3416Btlr/Mmmh
Stock Number:
040634-MU
Citation ID:
RRID:MMRRC_040634-MU
Other Names:
R3416 (G1), C57BL/6J-MtgxR3416Btlr
Major Collection:

Gene Information

Fryl
Name: FRY like transcription coactivator
Synonyms: 2510002A14Rik, 9030227G01Rik, 2310004H21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72313
Homologene: 103956
Adgrl2
Name: adhesion G protein-coupled receptor L2
Synonyms: Lphh1, Lphn2, Lec1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99633
Homologene: 22712
Dlgap5
Name: DLG associated protein 5
Synonyms: C86398, Hurp, Dlg7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218977
Homologene: 8840
Smg1
Name: SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)
Synonyms: 5430435M13Rik, 2610207I05Rik, C130002K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233789
Homologene: 56697
Abi1
Name: abl-interactor 1
Synonyms: Ssh3bp1, E3B1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11308
Homologene: 38053
Azin1
Name: antizyme inhibitor 1
Synonyms: ODC antizyme inhibitor, Oazi, Oazin, 1700085L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 54375
Homologene: 22933
Ralgapa1
Name: Ral GTPase activating protein, alpha subunit 1
Synonyms: 2310003F20Rik, Garnl1, 4930400K19Rik, Tulip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 56784
VEGA: 12
Homologene: 84805
Adnp2
Name: ADNP homeobox 2
Synonyms: 8430420L05Rik, Zfp508
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240442
Homologene: 8945
1500009L16Rik
Name: RIKEN cDNA 1500009L16 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 69784
VEGA: 10
Homologene: 136237
Vrk3
Name: vaccinia related kinase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101568
Homologene: 9509
Strbp
Name: spermatid perinuclear RNA binding protein
Synonyms: C230082I21Rik, Spnr, 6430510M02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20744
Homologene: 7548
Scn4a
Name: sodium channel, voltage-gated, type IV, alpha
Synonyms: mH2, Nav1.4, SkM1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 110880
Homologene: 283
Tgm3
Name: transglutaminase 3, E polypeptide
Synonyms: TG E, we
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21818
Homologene: 20690
Cyb561d2
Name: cytochrome b-561 domain containing 2
Synonyms: Tsp10, 101F6 protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 56368
Homologene: 38261
Tas2r124
Name: taste receptor, type 2, member 124
Synonyms: Tas2r24, T2R24, mt2r50, mGR24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387351
Homologene: 87293
Zfp560
Name: zinc finger protein 560
Synonyms: 2310030G09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 434377
Homologene: 103820
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: PKHDL1, fibrocystin L, D86 mRNA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 192190
Homologene: 16332
Zan
Name: zonadhesin
Synonyms: Zan
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22635
Homologene: 124417
Klhl42
Name: kelch-like 42
Synonyms: Klhdc5, C230080I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232539
Homologene: 45842
Olfr508
Name: olfactory receptor 508
Synonyms: GA_x6K02T2PBJ9-10959726-10960658, MOR204-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258769
Homologene: 72039
Mfsd13a
Name: major facilitator superfamily domain containing 13a
Synonyms: 4930538D17Rik, 4930449A08Rik, Tmem180
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 75146
Homologene: 12592
Sla2
Name: Src-like-adaptor 2
Synonyms: SLAP-2, SLAP2, A930009E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 77799
Homologene: 49989
Prl8a8
Name: prolactin family 8, subfamily a, member 81
Synonyms: 1600032B14Rik, Prlpc3, PLP-Cgamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 74188
Homologene: 137338
Igsf9b
Name: immunoglobulin superfamily, member 9B
Synonyms: LOC235086, AI414108
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235086
Homologene: 19472
Cyp4f39
Name: cytochrome P450, family 4, subfamily f, polypeptide 39
Synonyms: 4732474A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 320997
VEGA: 17
Homologene: 69814
Crocc
Name: ciliary rootlet coiled-coil, rootletin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230872
Homologene: 16811
Vmn2r120
Name: vomeronasal 2, receptor 120
Synonyms: EG224916
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224916
Susd5
Name: sushi domain containing 5
Synonyms: LOC382111
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382111
VEGA: 9
Homologene: 19526
Gfra1
Name: glial cell line derived neurotrophic factor family receptor alpha 1
Synonyms: GFR alpha-1, GDNFR-alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 14585
HGNC: HGNC:4243
Homologene: 3855
Vps52
Name: VPS52 GARP complex subunit
Synonyms: ARE1, D430041K17Rik, tclw5, tcl-w5, Sacm2l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224705
Homologene: 5756
Spata1
Name: spermatogenesis associated 1
Synonyms: 4921536I21Rik, SP-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 70951
Homologene: 23356
Tha1
Name: threonine aldolase 1
Synonyms: GLY1, 1300017K07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 71776
Homologene: 57081
Mycs
Name: myc-like oncogene, s-myc protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 17870
Homologene: 7854
Rtl4
Name: retrotransposon Gag like 4
Synonyms: Mar4, Zcchc16, C230031A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 619287
Homologene: 53503
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 23,040,002 bp
  • C to G, chromosome 2 at 37,590,725 bp
  • G to A, chromosome 2 at 130,047,772 bp
  • G to A, chromosome 2 at 156,875,942 bp
  • A to T, chromosome 3 at 146,487,508 bp
  • T to C, chromosome 3 at 148,859,329 bp
  • G to A, chromosome 4 at 141,046,447 bp
  • T to C, chromosome 5 at 73,108,074 bp
  • T to A, chromosome 5 at 137,435,720 bp
  • A to T, chromosome 6 at 132,755,638 bp
  • G to A, chromosome 6 at 147,107,880 bp
  • C to T, chromosome 7 at 44,775,442 bp
  • G to A, chromosome 7 at 108,630,018 bp
  • G to C, chromosome 7 at 118,148,853 bp
  • A to T, chromosome 9 at 20,347,678 bp
  • G to A, chromosome 9 at 27,309,478 bp
  • A to G, chromosome 9 at 107,540,126 bp
  • A to G, chromosome 9 at 114,095,658 bp
  • T to C, chromosome 10 at 83,759,632 bp
  • A to T, chromosome 11 at 106,330,413 bp
  • T to C, chromosome 11 at 117,873,200 bp
  • T to A, chromosome 12 at 55,770,613 bp
  • A to T, chromosome 13 at 27,511,549 bp
  • A to G, chromosome 14 at 47,406,427 bp
  • A to T, chromosome 15 at 38,493,546 bp
  • A to G, chromosome 15 at 44,547,364 bp
  • T to C, chromosome 17 at 32,489,742 bp
  • T to A, chromosome 17 at 33,963,252 bp
  • T to A, chromosome 17 at 57,509,241 bp
  • T to C, chromosome 18 at 80,128,158 bp
  • C to T, chromosome 19 at 46,371,992 bp
  • T to C, chromosome 19 at 58,267,112 bp
  • C to T, chromosome X at 5,468,756 bp
  • C to T, chromosome X at 145,119,905 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3416 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040634-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.