Strain Name:
C57BL/6J-MtgxR3416Btlr/Mmmh
Stock Number:
040634-MU
Citation ID:
RRID:MMRRC_040634-MU
Other Names:
R3416 (G1), C57BL/6J-MtgxR3416Btlr
Major Collection:

Strain Information

Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Adgrl2
Name: adhesion G protein-coupled receptor L2
Synonyms: Lphh1, Lec1, Lphn2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99633
Homologene: 22712
Dlgap5
Name: DLG associated protein 5
Synonyms: Hurp, C86398, Dlg7
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218977
Homologene: 8840
Smg1
Name: SMG1 nonsense mediated mRNA decay associated PI3K related kinase
Synonyms: C130002K18Rik, 5430435M13Rik, 2610207I05Rik, SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233789
Homologene: 56697
Abi1
Name: abl interactor 1
Synonyms: E3B1, Ssh3bp1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11308
Homologene: 38053
Azin1
Name: antizyme inhibitor 1
Synonyms: ODC antizyme inhibitor, Oazi, 1700085L02Rik, Oazin
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 54375
Homologene: 22933
Ralgapa1
Name: Ral GTPase activating protein, alpha subunit 1
Synonyms: 4930400K19Rik, Tulip1, 2310003F20Rik, Garnl1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56784
VEGA: 12
Homologene: 84805
Adnp2
Name: ADNP homeobox 2
Synonyms: Zfp508, 8430420L05Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240442
Homologene: 8945
1500009L16Rik
Name: RIKEN cDNA 1500009L16 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 69784
VEGA: 10
Homologene: 136237
Vrk3
Name: vaccinia related kinase 3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101568
Homologene: 9509
Strbp
Name: spermatid perinuclear RNA binding protein
Synonyms: Spnr, 6430510M02Rik, C230082I21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20744
Homologene: 7548
Scn4a
Name: sodium channel, voltage-gated, type IV, alpha
Synonyms: Nav1.4, mH2, SkM1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 110880
Homologene: 283
Tgm3
Name: transglutaminase 3, E polypeptide
Synonyms: TG E, we
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21818
Homologene: 20690
Cyb561d2
Name: cytochrome b-561 domain containing 2
Synonyms: 101F6 protein, Tsp10
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56368
Homologene: 38261
Tas2r124
Name: taste receptor, type 2, member 124
Synonyms: Tas2r24, mGR24, mt2r50, T2R24
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 387351
Homologene: 87293
Zfp560
Name: zinc finger protein 560
Synonyms: 2310030G09Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 434377
Homologene: 103820
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: D86 mRNA, PKHDL1, fibrocystin L
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 192190
Homologene: 16332
Zan
Name: zonadhesin
Synonyms: Zan
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22635
Homologene: 124417
Klhl42
Name: kelch-like 42
Synonyms: C230080I20Rik, Klhdc5
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232539
Homologene: 45842
Or5p80
Name: olfactory receptor family 5 subfamily P member 80
Synonyms: GA_x6K02T2PBJ9-10959726-10960658, MOR204-6, Olfr508
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258769
Homologene: 72039
Mfsd13a
Name: major facilitator superfamily domain containing 13a
Synonyms: 4930449A08Rik, 4930538D17Rik, Tmem180
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 75146
Homologene: 12592
Sla2
Name: Src-like-adaptor 2
Synonyms: SLAP-2, SLAP2, A930009E21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 77799
Homologene: 49989
Prl8a8
Name: prolactin family 8, subfamily a, member 81
Synonyms: PLP-Cgamma, 1600032B14Rik, Prlpc3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 74188
Homologene: 137338
Igsf9b
Name: immunoglobulin superfamily, member 9B
Synonyms: LOC235086, AI414108
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235086
Homologene: 19472
Cyp4f39
Name: cytochrome P450, family 4, subfamily f, polypeptide 39
Synonyms: 4732474A20Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 320997
VEGA: 17
Homologene: 69814
Crocc
Name: ciliary rootlet coiled-coil, rootletin
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230872
Homologene: 16811
Vmn2r120
Name: vomeronasal 2, receptor 120
Synonyms: EG224916
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224916
Susd5
Name: sushi domain containing 5
Synonyms: LOC382111
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 382111
VEGA: 9
Homologene: 19526
Gfra1
Name: glial cell line derived neurotrophic factor family receptor alpha 1
Synonyms: GDNFR-alpha, GFR alpha-1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 14585
HGNC: HGNC:4243
Homologene: 3855
Vps52
Name: VPS52 GARP complex subunit
Synonyms: ARE1, tcl-w5, D430041K17Rik, Sacm2l, tclw5
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224705
Homologene: 5756
Spata1
Name: spermatogenesis associated 1
Synonyms: SP-2, 4921536I21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 70951
Homologene: 23356
Tha1
Name: threonine aldolase 1
Synonyms: GLY1, 1300017K07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71776
Homologene: 57081
Mycs
Name: myc-like oncogene, s-myc protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 17870
HGNC: HGNC:7559
Homologene: 7854
Rtl4
Name: retrotransposon Gag like 4
Synonyms: Mar4, C230031A03Rik, Zcchc16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 619287
Homologene: 53503
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 23,040,002 bp
  • C to G, chromosome 2 at 37,590,725 bp
  • G to A, chromosome 2 at 130,047,772 bp
  • G to A, chromosome 2 at 156,875,942 bp
  • A to T, chromosome 3 at 146,487,508 bp
  • T to C, chromosome 3 at 148,859,329 bp
  • G to A, chromosome 4 at 141,046,447 bp
  • T to C, chromosome 5 at 73,108,074 bp
  • T to A, chromosome 5 at 137,435,720 bp
  • A to T, chromosome 6 at 132,755,638 bp
  • G to A, chromosome 6 at 147,107,880 bp
  • C to T, chromosome 7 at 44,775,442 bp
  • G to A, chromosome 7 at 108,630,018 bp
  • G to C, chromosome 7 at 118,148,853 bp
  • A to T, chromosome 9 at 20,347,678 bp
  • G to A, chromosome 9 at 27,309,478 bp
  • A to G, chromosome 9 at 107,540,126 bp
  • A to G, chromosome 9 at 114,095,658 bp
  • T to C, chromosome 10 at 83,759,632 bp
  • A to T, chromosome 11 at 106,330,413 bp
  • T to C, chromosome 11 at 117,873,200 bp
  • T to A, chromosome 12 at 55,770,613 bp
  • A to T, chromosome 13 at 27,511,549 bp
  • A to G, chromosome 14 at 47,406,427 bp
  • A to T, chromosome 15 at 38,493,546 bp
  • A to G, chromosome 15 at 44,547,364 bp
  • T to C, chromosome 17 at 32,489,742 bp
  • T to A, chromosome 17 at 33,963,252 bp
  • T to A, chromosome 17 at 57,509,241 bp
  • T to C, chromosome 18 at 80,128,158 bp
  • C to T, chromosome 19 at 46,371,992 bp
  • T to C, chromosome 19 at 58,267,112 bp
  • C to T, chromosome X at 5,468,756 bp
  • C to T, chromosome X at 145,119,905 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3416 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040634-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.