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Strain Name:
C57BL/6J-MtgxR3418Btlr/Mmmh
Stock Number:
040636-MU
Citation ID:
RRID:MMRRC_040636-MU
Other Names:
R3418 (G1), C57BL/6J-MtgxR3418Btlr
Major Collection:

Strain Information

Nos2
Name: nitric oxide synthase 2, inducible
Synonyms: iNOS, Nos-2, NOS-II, Nos2a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18126
HGNC: HGNC:7873
Homologene: 55473
Ubp1
Name: upstream binding protein 1
Synonyms: NF2d9, LBP-1b, LBP-1a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22221
VEGA: 9
Homologene: 8435
Ranbp17
Name: RAN binding protein 17
Synonyms: 4932704E15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66011
Homologene: 36409
Smc2
Name: structural maintenance of chromosomes 2
Synonyms: 5730502P04Rik, CAP-E, Fin16, Smc2l1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14211
Homologene: 4705
Armc9
Name: armadillo repeat containing 9
Synonyms: 5730415N24Rik, 3830422A13Rik, 4831423D23Rik, 4930438O05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 78795
Homologene: 11847
Dido1
Name: death inducer-obliterator 1
Synonyms: DIO-1, 6720461J16Rik, D130048F08Rik, Datf1, dido, C130092D22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 23856
HGNC: HGNC:2680
Homologene: 34139
Fdft1
Name: farnesyl diphosphate farnesyl transferase 1
Synonyms: squalene synthase, SQS, Ss
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 14137
VEGA: 14
HGNC: HGNC:3629
Homologene: 3281
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 14,874,381 bp
  • T to C, chromosome 1 at 86,194,338 bp
  • T to C, chromosome 1 at 87,386,919 bp
  • T to C, chromosome 1 at 90,804,091 bp
  • A to G, chromosome 1 at 105,809,405 bp
  • T to C, chromosome 1 at 106,998,927 bp
  • A to G, chromosome 2 at 76,379,018 bp
  • T to C, chromosome 2 at 125,320,926 bp
  • A to T, chromosome 2 at 178,401,653 bp
  • G to T, chromosome 2 at 180,660,935 bp
  • T to C, chromosome 3 at 61,003,712 bp
  • A to T, chromosome 3 at 64,116,899 bp
  • A to G, chromosome 3 at 75,640,282 bp
  • A to G, chromosome 3 at 82,106,133 bp
  • A to G, chromosome 3 at 137,892,870 bp
  • A to T, chromosome 4 at 3,746,833 bp
  • T to C, chromosome 4 at 25,211,252 bp
  • T to C, chromosome 4 at 52,476,850 bp
  • T to C, chromosome 5 at 30,904,723 bp
  • A to T, chromosome 5 at 138,757,868 bp
  • A to T, chromosome 6 at 71,883,486 bp
  • A to G, chromosome 6 at 129,421,038 bp
  • A to T, chromosome 6 at 132,115,677 bp
  • A to G, chromosome 6 at 133,294,119 bp
  • T to C, chromosome 6 at 135,843,110 bp
  • G to A, chromosome 7 at 43,692,077 bp
  • C to A, chromosome 7 at 139,017,745 bp
  • C to T, chromosome 8 at 13,769,489 bp
  • T to G, chromosome 8 at 15,085,294 bp
  • T to C, chromosome 8 at 69,302,927 bp
  • C to A, chromosome 8 at 71,645,959 bp
  • T to C, chromosome 8 at 91,036,591 bp
  • C to T, chromosome 8 at 104,129,370 bp
  • C to T, chromosome 9 at 37,537,130 bp
  • T to C, chromosome 9 at 113,951,686 bp
  • T to C, chromosome 9 at 116,129,833 bp
  • A to G, chromosome 9 at 119,349,490 bp
  • G to A, chromosome 10 at 7,907,481 bp
  • C to A, chromosome 10 at 74,584,222 bp
  • C to G, chromosome 10 at 127,286,503 bp
  • A to T, chromosome 11 at 33,289,552 bp
  • A to T, chromosome 11 at 34,689,760 bp
  • G to T, chromosome 11 at 55,278,998 bp
  • A to T, chromosome 11 at 58,156,628 bp
  • G to T, chromosome 11 at 68,893,156 bp
  • G to A, chromosome 11 at 74,053,988 bp
  • A to G, chromosome 11 at 78,400,840 bp
  • T to A, chromosome 11 at 78,959,695 bp
  • C to A, chromosome 12 at 76,887,888 bp
  • A to G, chromosome 12 at 91,810,002 bp
  • A to G, chromosome 12 at 95,780,604 bp
  • C to T, chromosome 12 at 112,738,468 bp
  • T to C, chromosome 14 at 63,156,621 bp
  • T to C, chromosome 15 at 69,049,375 bp
  • C to A, chromosome 15 at 100,580,924 bp
  • A to G, chromosome 16 at 5,074,379 bp
  • A to G, chromosome 16 at 32,299,192 bp
  • G to A, chromosome 16 at 64,925,897 bp
  • C to T, chromosome 16 at 73,035,917 bp
  • A to G, chromosome 19 at 37,922,978 bp
  • T to C, chromosome 19 at 47,857,905 bp
  • T to A, chromosome 19 at 56,831,231 bp
  • A to G, chromosome X at 70,353,284 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3418 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040636-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.