Strain Name:
C57BL/6J-MtgxR3420Btlr/Mmmh
Stock Number:
040638-MU
Citation ID:
RRID:MMRRC_040638-MU
Other Names:
R3420 (G1), C57BL/6J-MtgxR3420Btlr
Major Collection:

Gene Information

Slco1a5
Name: solute carrier organic anion transporter family, member 1a5
Synonyms: Oatp3, Slc21a7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 108096
Homologene: 56603
Kifap3
Name: kinesin-associated protein 3
Synonyms: KAP3, Smg GDS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16579
Homologene: 7799
Psmb2
Name: proteasome (prosome, macropain) subunit, beta type 2
Synonyms: D4Wsu33e, HC7-I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 26445
HGNC: HGNC:9539
Homologene: 2088
Ckap5
Name: cytoskeleton associated protein 5
Synonyms: 3110043H24Rik, D730027C18Rik, 4930432B04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 75786
Homologene: 8844
Slc25a17
Name: solute carrier family 25 (mitochondrial carrier, peroxisomal membrane protein), member 17
Synonyms: PMP34, 34kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20524
VEGA: 15
Homologene: 4637
Ric1
Name: RAB6A GEF complex partner 1
Synonyms: C030046E11Rik, C130057E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226089
Homologene: 13806
Tlr4
Name: toll-like receptor 4
Synonyms: Rasl2-8, lipopolysaccharide response, Lps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21898
Homologene: 41317
Grin1
Name: glutamate receptor, ionotropic, NMDA1 (zeta 1)
Synonyms: NR1, Nmdar, Rgsc174, NMDAR1, GluRzeta1, M100174
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14810
HGNC: HGNC:4584
Homologene: 7187
Klb
Name: klotho beta
Synonyms: betaKlotho
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 83379
Homologene: 12820
Ccdc73
Name: coiled-coil domain containing 73
Synonyms: 2210415I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 211936
Homologene: 52235
Cyp2b9
Name: cytochrome P450, family 2, subfamily b, polypeptide 9
Synonyms: phenobarbitol inducible, type a, Cyp2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13094
Homologene: 74933
Celsr2
Name: cadherin, EGF LAG seven-pass G-type receptor 2
Synonyms: flamingo, Adgrc2, mfmi1, EGFL2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 53883
HGNC: HGNC:3231
Homologene: 1078
Dclre1b
Name: DNA cross-link repair 1B
Synonyms: mSNM1B, Apollo, SNMIB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 140917
Homologene: 32553
Agbl3
Name: ATP/GTP binding protein-like 3
Synonyms: Ccp3, Ccp3, 2900053G10Rik, 6530406M24Rik, 4930431N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 76223
Homologene: 35330
Slc5a4a
Name: solute carrier family 5, member 4a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 64452
VEGA: 10
Zfp217
Name: zinc finger protein 217
Synonyms: 4933431C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228913
Homologene: 4757
Kcnip1
Name: Kv channel-interacting protein 1
Synonyms: KCHIP1, 2900046L02Rik, 3202002F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70357
Homologene: 22824
Amn1
Name: antagonist of mitotic exit network 1
Synonyms: C730024G19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232566
Homologene: 45811
Zdhhc14
Name: zinc finger, DHHC domain containing 14
Synonyms: New1cp, B530001K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224454
VEGA: 17
Homologene: 62301
Enthd1
Name: ENTH domain containing 1
Synonyms: LOC383075
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 383075
VEGA: 15
Homologene: 45131
Mrc2
Name: mannose receptor, C type 2
Synonyms: Endo180, novel lectin, uPARAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17534
Homologene: 4408
Washc1
Name: WASH complex subunit 1
Synonyms: Wash, 1110049F14Rik, Wash1, ORF19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 68767
VEGA: 17
Homologene: 45706
Prss23
Name: protease, serine 23
Synonyms: 2310046G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 76453
Homologene: 5191
Nuak2
Name: NUAK family, SNF1-like kinase, 2
Synonyms: Snark, 1200013B22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74137
Homologene: 12539
Olfr26
Name: olfactory receptor 26
Synonyms: MOR171-9, GA_x6K02T2PVTD-32550930-32551856, MTPCR09
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18324
VEGA: 9
HGNC: HGNC:8481
Homologene: 79676
Hoxc6
Name: homeobox C6
Synonyms: Hox-3.3, Hox-6.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 15425
HGNC: HGNC:5128
Homologene: 19533
Gm11787
Name: predicted gene 11787
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 666513
Tafa1
Name: TAFA chemokine like family member 1
Synonyms: Tafa-1, Fam19a1, C630007B19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320265
Homologene: 45655
Slc7a3
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 3
Synonyms: Atrc3, SLC7A2, SLC7A1, Cat3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 11989
Homologene: 56382
Prps2
Name: phosphoribosyl pyrophosphate synthetase 2
Synonyms: Prps-2, 2610101M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 110639
HGNC: HGNC:9465
Homologene: 128301
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 132,332,080 bp
  • T to A, chromosome 1 at 163,794,026 bp
  • C to T, chromosome 2 at 25,303,914 bp
  • T to A, chromosome 2 at 91,570,252 bp
  • A to T, chromosome 2 at 104,951,947 bp
  • G to A, chromosome 2 at 104,951,948 bp
  • A to G, chromosome 2 at 170,120,017 bp
  • A to G, chromosome 3 at 103,808,096 bp
  • A to G, chromosome 3 at 108,414,416 bp
  • A to G, chromosome 4 at 3,516,370 bp
  • A to G, chromosome 4 at 66,839,536 bp
  • T to C, chromosome 4 at 126,677,837 bp
  • G to A, chromosome 5 at 65,372,142 bp
  • A to G, chromosome 6 at 34,793,965 bp
  • C to A, chromosome 6 at 96,649,138 bp
  • A to T, chromosome 6 at 142,268,238 bp
  • A to T, chromosome 6 at 149,169,452 bp
  • G to A, chromosome 7 at 26,210,103 bp
  • C to A, chromosome 7 at 89,509,899 bp
  • A to G, chromosome 9 at 38,855,325 bp
  • T to C, chromosome 10 at 76,176,573 bp
  • A to G, chromosome 11 at 33,645,594 bp
  • G to A, chromosome 11 at 105,348,431 bp
  • T to C, chromosome 15 at 80,560,024 bp
  • C to T, chromosome 15 at 81,360,700 bp
  • T to A, chromosome 15 at 103,010,895 bp
  • T to A, chromosome 17 at 5,753,091 bp
  • T to C, chromosome 17 at 66,117,033 bp
  • T to C, chromosome 19 at 29,567,590 bp
  • T to A, chromosome X at 101,080,875 bp
  • G to A, chromosome X at 167,382,508 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3420 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040638-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.