Strain Name:
C57BL/6J-MtgxR3422Btlr/Mmmh
Stock Number:
040640-MU
Citation ID:
RRID:MMRRC_040640-MU
Other Names:
R3422 (G1), C57BL/6J-MtgxR3422Btlr
Major Collection:

Gene Information

Kifap3
Name: kinesin-associated protein 3
Synonyms: KAP3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16579
Homologene: 7799
Psmb2
Name: proteasome (prosome, macropain) subunit, beta type 2
Synonyms: HC7-I, D4Wsu33e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 26445
HGNC: HGNC:9539
Homologene: 2088
Ckap5
Name: cytoskeleton associated protein 5
Synonyms: 3110043H24Rik, 4930432B04Rik, D730027C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 75786
Homologene: 8844
Tlr4
Name: toll-like receptor 4
Synonyms: Lps, Rasl2-8, lipopolysaccharide response
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21898
Homologene: 41317
Sarnp
Name: SAP domain containing ribonucleoprotein
Synonyms: 1110005A23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 66118
VEGA: 10
Homologene: 41628
Brca2
Name: breast cancer 2, early onset
Synonyms: RAB163, Fancd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12190
HGNC: HGNC:1101
Homologene: 41
Grin1
Name: glutamate receptor, ionotropic, NMDA1 (zeta 1)
Synonyms: NMDAR1, NR1, Nmdar, M100174, GluRzeta1, Rgsc174
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14810
HGNC: HGNC:4584
Homologene: 7187
Me2
Name: malic enzyme 2, NAD(+)-dependent, mitochondrial
Synonyms: D030040L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 107029
VEGA: 18
HGNC: HGNC:6984
Homologene: 37615
Spink5
Name: serine peptidase inhibitor, Kazal type 5
Synonyms: LEKT1, 2310065D10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 72432
Homologene: 4987
Soat2
Name: sterol O-acyltransferase 2
Synonyms: ACAT2, D15Wsu97e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223920
Homologene: 68355
Ccdc73
Name: coiled-coil domain containing 73
Synonyms: 2210415I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 211936
Homologene: 52235
Vsig2
Name: V-set and immunoglobulin domain containing 2
Synonyms: CTM, CTX, 1190004B15Rik, 2210413P10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 57276
VEGA: 9
Homologene: 8630
Atp7b
Name: ATPase, Cu++ transporting, beta polypeptide
Synonyms: WND, Wilson protein, Atp7a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11979
HGNC: HGNC:870
Homologene: 20063
Agbl3
Name: ATP/GTP binding protein-like 3
Synonyms: 2900053G10Rik, 6530406M24Rik, 4930431N21Rik, Ccp3, Ccp3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 76223
Homologene: 35330
Slc5a4a
Name: solute carrier family 5, member 4a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 64452
VEGA: 10
Ahnak
Name: AHNAK nucleoprotein (desmoyokin)
Synonyms: DY6, 2310047C17Rik, 1110004P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 66395
VEGA: 19
HGNC: HGNC:347
Homologene: 67425
Odad2
Name: outer dynein arm docking complex subunit 2
Synonyms: 4930463I21Rik, Armc4, b2b643Clo, b2b227.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 74934
VEGA: 18
Homologene: 9992
Zfp217
Name: zinc finger protein 217
Synonyms: 4933431C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228913
Homologene: 4757
Pik3cg
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma
Synonyms: PI3Kgamma, p110gamma, PI3Kgamma, PI(3)Kgamma, 5830428L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 30955
HGNC: HGNC:8978
Homologene: 68269
Nwd2
Name: NACHT and WD repeat domain containing 2
Synonyms: B830017A01Rik, 3110047P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 319807
Homologene: 14974
Zfp91
Name: zinc finger protein 91
Synonyms: Pzf, 9130014I08Rik, A530054C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 109910
Homologene: 43144
Kcnip1
Name: Kv channel-interacting protein 1
Synonyms: 2900046L02Rik, KCHIP1, 3202002F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70357
Homologene: 22824
Mrc2
Name: mannose receptor, C type 2
Synonyms: novel lectin, Endo180, uPARAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17534
Homologene: 4408
Nmt2
Name: N-myristoyltransferase 2
Synonyms: hNMT-2, A930001K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18108
HGNC: HGNC:7858
Homologene: 101539
Olfr725
Name: olfactory receptor 725
Synonyms: GA_x6K02T2PMLR-5725741-5724776, MOR246-3, MOR246-7_p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258314
Homologene: 44957
Ifit1bl1
Name: interferon induced protein with tetratricpeptide repeats 1B like 1
Synonyms: Gm14446
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 667373
Homologene: 78213
D930048N14Rik
Name: RIKEN cDNA D930048N14 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 97775
Mgat4d
Name: MGAT4 family, member C
Synonyms: 4933434I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67555
Homologene: 128538
Nuak2
Name: NUAK family, SNF1-like kinase, 2
Synonyms: 1200013B22Rik, Snark
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74137
Homologene: 12539
Olfr20
Name: olfactory receptor 20
Synonyms: MTPCR55, MTPCR06, Olfr21, MOR135-11, GA_x6K02T2P1NL-3514066-3515010
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258925
Homologene: 128054
Otub1
Name: OTU domain, ubiquitin aldehyde binding 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107260
Homologene: 9772
Saysd1
Name: SAYSVFN motif domain containing 1
Synonyms: 4930488P03Rik, 1810063B07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67509
VEGA: 14
Homologene: 41248
Tafa1
Name: TAFA chemokine like family member 1
Synonyms: Tafa-1, C630007B19Rik, Fam19a1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320265
Homologene: 45655
Slc7a3
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 3
Synonyms: SLC7A2, SLC7A1, Cat3, Atrc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 11989
Homologene: 56382
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 132,332,080 bp
  • T to A, chromosome 1 at 163,794,026 bp
  • A to G, chromosome 2 at 3,284,388 bp
  • C to T, chromosome 2 at 25,303,914 bp
  • T to A, chromosome 2 at 91,570,252 bp
  • A to T, chromosome 2 at 104,951,947 bp
  • G to A, chromosome 2 at 104,951,948 bp
  • A to G, chromosome 2 at 170,120,017 bp
  • A to G, chromosome 4 at 66,839,536 bp
  • T to C, chromosome 4 at 126,677,837 bp
  • G to T, chromosome 5 at 63,725,193 bp
  • A to G, chromosome 5 at 150,543,121 bp
  • A to G, chromosome 6 at 34,793,965 bp
  • C to A, chromosome 6 at 96,649,138 bp
  • C to T, chromosome 8 at 22,028,670 bp
  • T to C, chromosome 8 at 83,358,143 bp
  • G to A, chromosome 9 at 37,541,479 bp
  • T to C, chromosome 10 at 76,176,573 bp
  • G to A, chromosome 10 at 128,877,326 bp
  • A to G, chromosome 11 at 33,645,594 bp
  • T to C, chromosome 11 at 51,654,958 bp
  • A to G, chromosome 11 at 73,354,634 bp
  • G to A, chromosome 11 at 105,348,431 bp
  • A to T, chromosome 12 at 32,204,739 bp
  • T to A, chromosome 14 at 20,082,926 bp
  • T to C, chromosome 14 at 50,034,540 bp
  • T to C, chromosome 15 at 102,156,809 bp
  • T to C, chromosome 18 at 7,223,523 bp
  • A to G, chromosome 18 at 44,010,244 bp
  • C to T, chromosome 18 at 73,791,194 bp
  • C to T, chromosome 19 at 7,199,059 bp
  • A to G, chromosome 19 at 9,005,708 bp
  • A to G, chromosome 19 at 9,006,752 bp
  • G to A, chromosome 19 at 12,770,292 bp
  • T to G, chromosome 19 at 34,593,950 bp
  • T to A, chromosome X at 101,080,875 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3422 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040640-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.