Strain Name:
C57BL/6J-MtgxR3424Btlr/Mmmh
Stock Number:
040642-MU
Citation ID:
RRID:MMRRC_040642-MU
Other Names:
R3424 (G1), C57BL/6J-MtgxR3424Btlr
Major Collection:

Strain Information

Galnt10
Name: polypeptide N-acetylgalactosaminyltransferase 10
Synonyms: GalNAc-T10, Galnt9, C330012K04Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 171212
Homologene: 14924
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, Macf2, bullous pemphigoid antigen 1, athetoid, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Oxtr
Name: oxytocin receptor
Synonyms: OTR
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 18430
HGNC: HGNC:8529
Homologene: 20255
Mysm1
Name: myb-like, SWIRM and MPN domains 1
Synonyms: C530050H10Rik, C130067A03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320713
Homologene: 66204
Golga4
Name: golgin A4
Synonyms: golgin-245, Olp-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 54214
HGNC: HGNC:4427
Homologene: 68224
Virma
Name: vir like m6A methyltransferase associated
Synonyms: 1110037F02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66185
Homologene: 41043
Tfcp2l1
Name: transcription factor CP2-like 1
Synonyms: LBP-9, Crtr-1, 4932442M07Rik, D930018N21Rik, 1810030F05Rik, Cp2l1, Tcfcp2l1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 81879
Homologene: 8730
Arhgap32
Name: Rho GTPase activating protein 32
Synonyms: GC-GAP, p200RhoGAP, PX-RICS, 3426406O18Rik, Grit
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330914
Homologene: 8812
Zbtb17
Name: zinc finger and BTB domain containing 17
Synonyms: mZ13, Miz1, Zfp100
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22642
Homologene: 2575
Hspg2
Name: perlecan (heparan sulfate proteoglycan 2)
Synonyms: per, Pcn, Plc, perlecan
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15530
HGNC: HGNC:5273
Homologene: 68473
Paxip1
Name: PAX interacting (with transcription-activation domain) protein 1
Synonyms: PTIP, D5Ertd149e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 55982
HGNC: HGNC:8624
Heatr5b
Name: HEAT repeat containing 5B
Synonyms: D330050P16Rik, 2010013B10Rik, A230048G03Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 320473
VEGA: 17
Homologene: 25536
Sag
Name: S-antigen, retina and pineal gland (arrestin)
Synonyms: A930001K18Rik, rod arrestin, Arr1, arrestin 1, visual arrestin 1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20215
Homologene: 455
Cdh23
Name: cadherin related 23 (otocadherin)
Synonyms: 4930542A03Rik, USH1D, mdfw, ahl, bob, nmf112, nmf181, nmf252, sals
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22295
Homologene: 11142
Hoxd4
Name: homeobox D4
Synonyms: Hox-4.2, Hox-5.1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 15436
HGNC: HGNC:5138
Homologene: 7773
Pclo
Name: piccolo (presynaptic cytomatrix protein)
Synonyms: Acz, Pico
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 26875
Homologene: 69111
Csf3r
Name: colony stimulating factor 3 receptor
Synonyms: G-CSFR, Csfgr, Cd114
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12986
HGNC: HGNC:2439
Homologene: 601
Cdc16
Name: CDC16 cell division cycle 16
Synonyms: 2810431D22Rik, 2700071J12Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 69957
HGNC: HGNC:1720
Homologene: 2899
BC016579
Name: cDNA sequence, BC016579
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 212998
VEGA: 16
Homologene: 11622
Ros1
Name: Ros1 proto-oncogene
Synonyms: Ros-1, c-ros
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19886
Homologene: 2207
Hps1
Name: HPS1, biogenesis of lysosomal organelles complex 3 subunit 1
Synonyms: 6030422N11Rik, Hermansky-Pudlak syndrome 1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 192236
HGNC: HGNC:5163
Homologene: 163
Gabrr1
Name: gamma-aminobutyric acid type A receptor subunit rho 1
Synonyms: GABA-C
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14408
HGNC: HGNC:4090
Homologene: 20470
Lrrc8e
Name: leucine rich repeat containing 8 family, member E
Synonyms: C87354, 1810049O03Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 72267
Homologene: 11817
Atp8b3
Name: ATPase, class I, type 8B, member 3
Synonyms: SAPLT, 1700042F02Rik, 1700056N23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67331
VEGA: 10
Homologene: 19034
Slco4c1
Name: solute carrier organic anion transporter family, member 4C1
Synonyms: SLC21A20, PRO2176, OATP4C1, OATP-M1, OATP-H, C330017E21Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227394
Homologene: 62654
Cep250
Name: centrosomal protein 250
Synonyms: Inmp, B230210E21Rik, Cep2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16328
HGNC: HGNC:1859
Homologene: 38286
Mettl24
Name: methyltransferase like 24
Synonyms: 9030224M15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 327747
Homologene: 65335
6030469F06Rik
Name: RIKEN cDNA 6030469F06 gene
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 100043065
VEGA: 12
Coro2b
Name: coronin, actin binding protein, 2B
Synonyms: E130012P22Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235431
HGNC: HGNC:2256
Homologene: 21228
Slc18b1
Name: solute carrier family 18, subfamily B, member 1
Synonyms: 1110021L09Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 76306
Homologene: 33473
Tspan31
Name: tetraspanin 31
Synonyms: 2700085A14Rik, Tspan31, Sas
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67125
VEGA: 10
Homologene: 4359
Or10g9
Name: olfactory receptor family 10 subfamily G member 9
Synonyms: GA_x6K02T2PVTD-33699706-33698771, MOR223-1, Olfr979
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 259112
VEGA: 9
Homologene: 81556
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 34,198,505 bp
  • T to C, chromosome 1 at 87,810,295 bp
  • A to T, chromosome 1 at 96,841,251 bp
  • T to C, chromosome 1 at 118,652,980 bp
  • A to G, chromosome 2 at 74,727,313 bp
  • G to A, chromosome 2 at 155,981,461 bp
  • G to T, chromosome 4 at 11,513,177 bp
  • T to C, chromosome 4 at 33,158,058 bp
  • A to G, chromosome 4 at 94,965,321 bp
  • A to G, chromosome 4 at 126,043,756 bp
  • C to T, chromosome 4 at 137,511,380 bp
  • G to T, chromosome 4 at 141,464,988 bp
  • C to T, chromosome 5 at 14,680,418 bp
  • A to G, chromosome 5 at 27,775,673 bp
  • G to T, chromosome 6 at 112,477,230 bp
  • A to T, chromosome 8 at 4,234,611 bp
  • A to G, chromosome 8 at 13,763,808 bp
  • G to A, chromosome 9 at 32,247,642 bp
  • T to A, chromosome 9 at 40,000,534 bp
  • A to T, chromosome 9 at 62,429,308 bp
  • A to C, chromosome 9 at 118,534,647 bp
  • T to G, chromosome 10 at 23,822,976 bp
  • C to T, chromosome 10 at 40,683,641 bp
  • C to A, chromosome 10 at 52,128,416 bp
  • T to G, chromosome 10 at 60,376,881 bp
  • T to C, chromosome 10 at 80,536,347 bp
  • T to A, chromosome 10 at 127,068,502 bp
  • T to C, chromosome 11 at 57,645,713 bp
  • G to T, chromosome 12 at 31,184,633 bp
  • T to A, chromosome 16 at 45,629,483 bp
  • G to A, chromosome 17 at 78,768,404 bp
  • A to T, chromosome 19 at 42,760,513 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3424 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040642-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.