Strain Name:
C57BL/6J-MtgxR3499Btlr/Mmmh
Stock Number:
040662-MU
Citation ID:
RRID:MMRRC_040662-MU
Other Names:
R3499 (G1), C57BL/6J-MtgxR3499Btlr
Major Collection:

Strain Information

Ptprf
Name: protein tyrosine phosphatase, receptor type, F
Synonyms: LAR, RPTP-LAR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 19268
HGNC: HGNC:9670
Homologene: 20623
Ranbp17
Name: RAN binding protein 17
Synonyms: 4932704E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66011
Homologene: 36409
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Spred1
Name: sprouty protein with EVH-1 domain 1, related sequence
Synonyms: Spred-1, 5730461F13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 114715
Homologene: 24919
Ebag9
Name: estrogen receptor-binding fragment-associated gene 9
Synonyms: Rcas1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 55960
VEGA: 15
HGNC: HGNC:3123
Homologene: 3107
Cad
Name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Synonyms: 2410008J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 69719
HGNC: HGNC:1424
Homologene: 1412
Trip11
Name: thyroid hormone receptor interactor 11
Synonyms: 3110031G15Rik, 2610511G22Rik, 6030460N08Rik, TRIP230, GMAP-210
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 109181
Homologene: 20897
Tasor
Name: transcription activation suppressor
Synonyms: 4933409E02Rik, D14Abb1e, MommeD6, Fam208a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218850
VEGA: 14
Homologene: 9062
Ralgapa1
Name: Ral GTPase activating protein, alpha subunit 1
Synonyms: 4930400K19Rik, Tulip1, 2310003F20Rik, Garnl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 56784
VEGA: 12
Homologene: 84805
Smyd5
Name: SET and MYND domain containing 5
Synonyms: NN8-4AG, Rrg1, Rai15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232187
Homologene: 6143
Cand1
Name: cullin associated and neddylation disassociated 1
Synonyms: 6330512O03Rik, 2310038O07Rik, D10Ertd516e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 71902
Homologene: 10202
Ino80d
Name: INO80 complex subunit D
Synonyms: A430093A21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227195
Homologene: 9819
Nucb1
Name: nucleobindin 1
Synonyms: MTEST82, B230337F23Rik, Calnuc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18220
HGNC: HGNC:8043
Homologene: 4507
Kif13a
Name: kinesin family member 13A
Synonyms: N-3 kinesin, 4930505I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 16553
VEGA: 13
Homologene: 22589
Kirrel
Name: kirre like nephrin family adhesion molecule 1
Synonyms: Neph1, Kirrel1, 6720469N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 170643
Homologene: 10089
Tab3
Name: TGF-beta activated kinase 1/MAP3K7 binding protein 3
Synonyms: 4921526G09Rik, Map3k7ip3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 66724
Homologene: 17673
Slc44a4
Name: solute carrier family 44, member 4
Synonyms: NG22, 2210409B01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 70129
Homologene: 11359
Hmcn1
Name: hemicentin 1
Synonyms: LOC240793, EG545370
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545370
Homologene: 23741
Dnah6
Name: dynein, axonemal, heavy chain 6
Synonyms: A730004I20Rik, Dnahc6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330355
HGNC: HGNC:2951
Homologene: 15221
Syne2
Name: spectrin repeat containing, nuclear envelope 2
Synonyms: nesprin-2, syne-2, D12Ertd777e, 6820443O06Rik, Nesp2g, Cpfl8, diminished cone electroretinogram, dice
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 319565
Homologene: 56700
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: lrd, b2b598Clo, b2b1289Clo, b2b1727Clo, b2b1279Clo, b2b1203Clo, Dnahc11, avc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Dnah17
Name: dynein, axonemal, heavy chain 17
Synonyms: LOC382552, 2810003K23Rik, Dnahcl1, Dnahc17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69926
HGNC: HGNC:2946
Homologene: 72102
Gpr35
Name: G protein-coupled receptor 35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 64095
HGNC: HGNC:4492
Homologene: 3874
Ephb6
Name: Eph receptor B6
Synonyms: Mep, Cekl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 13848
HGNC: HGNC:3396
Homologene: 20940
9530053A07Rik
Name: RIKEN cDNA 9530053A07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 319482
Homologene: 130055
Ttf1
Name: transcription termination factor, RNA polymerase I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22130
Homologene: 137223
Cass4
Name: Cas scaffolding protein family member 4
Synonyms: F730031O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320664
Homologene: 75128
6430571L13Rik
Name: RIKEN cDNA 6430571L13 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235599
Homologene: 9425
Slc6a9
Name: solute carrier family 6 (neurotransmitter transporter, glycine), member 9
Synonyms: Glyt-1, Glyt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14664
Homologene: 5050
Lyplal1
Name: lysophospholipase-like 1
Synonyms: Q96AVO
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226791
Homologene: 13683
Ghrhr
Name: growth hormone releasing hormone receptor
Synonyms: Ghrfr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14602
HGNC: HGNC:4266
Homologene: 640
Smgc
Name: submandibular gland protein C
Synonyms: Sfc21, 2310010P21Rik, DXImx49e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223809
Sla2
Name: Src-like-adaptor 2
Synonyms: SLAP-2, SLAP2, A930009E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 77799
Homologene: 49989
Or8h8
Name: olfactory receptor family 8 subfamily H member 8
Synonyms: GA_x6K02T2Q125-48410458-48409511, MOR206-1, Olfr1098
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258842
Homologene: 37005
Zfp395
Name: zinc finger protein 395
Synonyms: LOC380912, BC027382
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 380912
VEGA: 14
Homologene: 10255
Slc28a3
Name: solute carrier family 28 (sodium-coupled nucleoside transporter), member 3
Synonyms: Cnt3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 114304
Homologene: 32533
Ctif
Name: CBP80/20-dependent translation initiation factor
Synonyms: LOC269037, Gm672
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 269037
VEGA: 18
Homologene: 56682
Or56b35
Name: olfactory receptor family 56 subfamily B member 35
Synonyms: GA_x6K02T2PBJ9-7942985-7943947, MOR40-3, Olfr689
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258745
Homologene: 128074
Fndc1
Name: fibronectin type III domain containing 1
Synonyms: 1110027O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 68655
Tktl2
Name: transketolase-like 2
Synonyms: 4933401I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 74419
Homologene: 69456
Mrgprb5
Name: MAS-related GPR, member B5
Synonyms: MrgB5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 404239
Homologene: 115575
1700001C19Rik
Name: RIKEN cDNA 1700001C19 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 75462
Homologene: 129540
Tbc1d12
Name: TBC1D12: TBC1 domain family, member 12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 209478
VEGA: 19
Homologene: 50835
Or4a15
Name: olfactory receptor family 4 subfamily A member 15
Synonyms: GA_x6K02T2Q125-50805620-50804676, MOR231-2, Olfr1234
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258975
Homologene: 128156
Or10d1c
Name: olfactory receptor family 10 subfamily D member 1C
Synonyms: GA_x6K02T2PVTD-32678895-32677963, MOR224-6, Olfr934
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258434
VEGA: 9
Homologene: 51730
Tmem181a
Name: transmembrane protein 181A
Synonyms: 5930418K15Rik, Gpr178, C76977, Tmem181
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 77106
Homologene: 44787
Tmem229b-ps
Name: transmembrane protein 229B, pseudogene
Synonyms: Gm5423
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 432460
VEGA: 10
Or8k18
Name: olfactory receptor family 8 subfamily K member 18
Synonyms: GA_x6K02T2Q125-47735396-47734470, MOR187-1, Olfr1049
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 259018
Homologene: 77387
Elf4
Name: E74-like factor 4 (ets domain transcription factor)
Synonyms: myeloid elf-1 like factor, MEF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 56501
HGNC: HGNC:3319
Homologene: 31036
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 63,061,210 bp
  • A to G, chromosome 1 at 92,983,391 bp
  • A to T, chromosome 1 at 150,605,102 bp
  • A to G, chromosome 1 at 186,088,660 bp
  • T to C, chromosome 2 at 29,065,487 bp
  • A to G, chromosome 2 at 86,254,841 bp
  • A to G, chromosome 2 at 86,923,029 bp
  • A to T, chromosome 2 at 89,362,950 bp
  • A to T, chromosome 2 at 117,175,386 bp
  • G to A, chromosome 2 at 156,875,942 bp
  • C to T, chromosome 2 at 172,432,558 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • A to G, chromosome 4 at 117,856,803 bp
  • A to T, chromosome 4 at 118,224,930 bp
  • T to C, chromosome 5 at 31,073,984 bp
  • C to T, chromosome 6 at 41,616,159 bp
  • A to G, chromosome 6 at 55,383,771 bp
  • G to C, chromosome 6 at 73,032,633 bp
  • T to A, chromosome 6 at 85,438,190 bp
  • T to A, chromosome 7 at 28,154,555 bp
  • T to C, chromosome 7 at 45,498,876 bp
  • A to G, chromosome 7 at 48,168,913 bp
  • G to A, chromosome 7 at 105,314,400 bp
  • T to A, chromosome 8 at 66,513,245 bp
  • A to G, chromosome 9 at 38,982,465 bp
  • A to T, chromosome 9 at 107,342,479 bp
  • C to T, chromosome 10 at 53,475,127 bp
  • T to C, chromosome 10 at 119,215,188 bp
  • GCCTGGATACTGACC to GCC, chromosome 11 at 33,219,203 bp
  • C to T, chromosome 11 at 118,080,849 bp
  • T to G, chromosome 12 at 55,695,143 bp
  • G to A, chromosome 12 at 76,054,978 bp
  • A to G, chromosome 12 at 101,893,694 bp
  • C to A, chromosome 12 at 117,911,023 bp
  • T to C, chromosome 13 at 46,825,339 bp
  • C to T, chromosome 13 at 58,573,439 bp
  • C to T, chromosome 14 at 27,441,989 bp
  • G to T, chromosome 14 at 65,391,293 bp
  • A to G, chromosome 15 at 44,630,132 bp
  • A to G, chromosome 15 at 91,842,003 bp
  • A to T, chromosome 16 at 15,768,025 bp
  • T to A, chromosome 17 at 6,280,619 bp
  • C to T, chromosome 17 at 7,753,584 bp
  • A to G, chromosome 17 at 34,921,680 bp
  • AC to A, chromosome 17 at 47,433,423 bp
  • A to T, chromosome 18 at 75,611,757 bp
  • A to T, chromosome 19 at 38,896,034 bp
  • C to T, chromosome X at 48,419,065 bp
  • T to C, chromosome X at 85,615,121 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3499 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040662-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.