Strain Name:
C57BL/6J-MtgxR3546Btlr/Mmmh
Stock Number:
040665-MU
Citation ID:
RRID:MMRRC_040665-MU
Other Names:
R3546 (G1), C57BL/6J-MtgxR3546Btlr
Major Collection:

Gene Information

Itk
Name: IL2 inducible T cell kinase
Synonyms: Emt, Tsk, Tcsk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16428
HGNC: HGNC:6171
Homologene: 4051
Utp20
Name: UTP20 small subunit processome component
Synonyms: DRIM, mDRIM, 3830408P06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70683
VEGA: 10
Homologene: 38373
Khdc4
Name: KH domain containing 4, pre-mRNA splicing factor
Synonyms: 2810403A07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 74200
Homologene: 41761
Ddx23
Name: DEAD box helicase 23
Synonyms: 4921506D17Rik, 3110082M05Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 74351
Homologene: 3542
Sult6b1
Name: sulfotransferase family, cytosolic, 6B, member 1
Synonyms: 2410078J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 73671
Homologene: 42242
Etaa1
Name: Ewing tumor-associated antigen 1
Synonyms: 5730466H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68145
Homologene: 10369
Clcc1
Name: chloride channel CLIC-like 1
Synonyms: Mclc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229725
Homologene: 9032
Cyth1
Name: cytohesin 1
Synonyms: CLM1, CTH-1, Pscd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 19157
HGNC: HGNC:9501
Homologene: 31262
Tbc1d1
Name: TBC1 domain family, member 1
Synonyms: 1110062G02Rik, Nob1, Nobq1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 57915
Homologene: 56856
Mei1
Name: meiotic double-stranded break formation protein 1
Synonyms: mei1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 74369
Homologene: 46535
Nbeal1
Name: neurobeachin like 1
Synonyms: A530050O19Rik, ALS2CR17, A530083I02Rik, 2310076G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 269198
Homologene: 16453
Ush1g
Name: USH1 protein network component sans
Synonyms: js, Sans
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16470
Homologene: 56113
Mslnl
Name: mesothelin-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 328783
VEGA: 17
Homologene: 18633
Reln
Name: reelin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Golga7b
Name: golgi autoantigen, golgin subfamily a, 7B
Synonyms: 4933417O08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 71146
Homologene: 28514
Slc16a7
Name: solute carrier family 16 (monocarboxylic acid transporters), member 7
Synonyms: MCT2, 4921534N07Rik, D630004K10Rik, 9030411M13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 20503
Homologene: 20990
Kdm1b
Name: lysine (K)-specific demethylase 1B
Synonyms: 4632428N09Rik, Aof1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218214
Homologene: 14695
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435626
Homologene: 52359
Kcnh7
Name: potassium voltage-gated channel, subfamily H (eag-related), member 7
Synonyms: Kv11.3, erg3, 9330137I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 170738
Homologene: 13249
Fap
Name: fibroblast activation protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14089
HGNC: HGNC:3590
Homologene: 48282
Hdac7
Name: histone deacetylase 7
Synonyms: 5830434K02Rik, Hdac7a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 56233
Homologene: 9124
AA986860
Name: expressed sequence AA986860
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 212439
Homologene: 49741
Olfr524
Name: olfactory receptor 524
Synonyms: GA_x6K02T2PBJ9-42354580-42353624, MOR103-14P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258055
Homologene: 79347
Piwil4
Name: piwi-like RNA-mediated gene silencing 4
Synonyms: 9230101H05Rik, Miwi2, MIWI2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 330890
Homologene: 45122
Ceacam1
Name: carcinoembryonic antigen-related cell adhesion molecule 1
Synonyms: CD66a, MHVR1, mmCGM1, mCEA1, mmCGM2, Hv-2, Bgp, Mhv-1, Cea-7, Cea-1, Cea1, Bgp1, C-CAM, Hv2, Cea7, Cc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26365
HGNC: HGNC:1814
Homologene: 128630
Vmn1r7
Name: vomeronasal 1 receptor 7
Synonyms: Gm5568, V1rc31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 434016
Homologene: 83427
Nlrp6
Name: NLR family, pyrin domain containing 6
Synonyms: Nalp6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101613
Homologene: 15881
Celf3
Name: CUGBP, Elav-like family member 3
Synonyms: BRUNOL1, ERDA4, CAGH4, 4930415M08Rik, Tnrc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 78784
Homologene: 48510
Pdilt
Name: protein disulfide isomerase-like, testis expressed
Synonyms: PDILT, 1700007B13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71830
Homologene: 18382
Bves
Name: blood vessel epicardial substance
Synonyms: Popdc1, popeye 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 23828
VEGA: 10
HGNC: HGNC:1152
Homologene: 48497
Ppp1r27
Name: protein phosphatase 1, regulatory subunit 27
Synonyms: toonin, 1110033I14Rik, Dysfip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68701
Homologene: 45933
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 60,278,780 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • A to G, chromosome 1 at 130,741,189 bp
  • A to T, chromosome 2 at 40,600,288 bp
  • A to C, chromosome 2 at 62,519,011 bp
  • A to G, chromosome 2 at 62,764,780 bp
  • T to A, chromosome 2 at 76,745,106 bp
  • A to G, chromosome 3 at 88,693,136 bp
  • T to G, chromosome 3 at 94,488,538 bp
  • T to A, chromosome 3 at 108,668,113 bp
  • A to G, chromosome 5 at 22,227,600 bp
  • G to A, chromosome 5 at 64,286,007 bp
  • A to G, chromosome 6 at 57,024,849 bp
  • T to C, chromosome 7 at 25,471,914 bp
  • C to A, chromosome 7 at 119,500,488 bp
  • A to G, chromosome 7 at 140,202,101 bp
  • T to G, chromosome 7 at 140,926,769 bp
  • T to C, chromosome 9 at 14,736,956 bp
  • A to G, chromosome 10 at 45,354,811 bp
  • T to C, chromosome 10 at 88,782,689 bp
  • T to A, chromosome 10 at 125,294,700 bp
  • A to G, chromosome 11 at 17,953,823 bp
  • A to G, chromosome 11 at 46,355,848 bp
  • T to A, chromosome 11 at 115,318,897 bp
  • A to G, chromosome 11 at 118,192,436 bp
  • T to A, chromosome 11 at 120,550,685 bp
  • C to T, chromosome 13 at 47,063,077 bp
  • A to G, chromosome 15 at 82,098,042 bp
  • G to T, chromosome 15 at 97,808,009 bp
  • G to A, chromosome 15 at 98,650,732 bp
  • T to C, chromosome 17 at 25,744,969 bp
  • G to A, chromosome 17 at 78,906,907 bp
  • T to C, chromosome 19 at 42,267,071 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3546 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040665-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.