Strain Name:
C57BL/6J-MtgxR3548Btlr/Mmmh
Stock Number:
040667-MU
Citation ID:
RRID:MMRRC_040667-MU
Other Names:
R3548 (G1), C57BL/6J-MtgxR3548Btlr
Major Collection:

Strain Information

Chrnb2
Name: cholinergic receptor nicotinic beta 2 subunit
Synonyms: Acrb2, [b]2-nAchR, Acrb-2, C030030P04Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11444
HGNC: HGNC:1962
Homologene: 595
Nes
Name: nestin
Synonyms: Ifaprc2, Marc2, ESTM46, RC2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18008
HGNC: HGNC:7756
Homologene: 136487
Sort1
Name: sortilin 1
Synonyms: Ntr3, 2900053A11Rik, sortilin, Ntsr3, neurotensin receptor 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20661
Homologene: 136097
Khdc4
Name: KH domain containing 4, pre-mRNA splicing factor
Synonyms: 2810403A07Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74200
Homologene: 41761
Sirt2
Name: sirtuin 2
Synonyms: 5730427M03Rik, Sir2l, SIR2L2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 64383
Homologene: 40823
Clcc1
Name: chloride channel CLIC-like 1
Synonyms: Mclc
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229725
Homologene: 9032
Ankrd28
Name: ankyrin repeat domain 28
Synonyms: E430019N21Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105522
VEGA: 14
Homologene: 35374
Bmal1
Name: basic helix-loop-helix ARNT like 1
Synonyms: bHLHe5, Arnt3, Arntl, MOP3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11865
HGNC: HGNC:701
Homologene: 910
Phf24
Name: PHD finger protein 24
Synonyms: GINIP, N28178
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230085
Homologene: 18208
Il17rb
Name: interleukin 17 receptor B
Synonyms: Il17br, Evi27, IL-17Rh1, IL-17ER, IL17RH1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 50905
Homologene: 10287
Amph
Name: amphiphysin
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218038
HGNC: HGNC:471
Homologene: 121585
Nid2
Name: nidogen 2
Synonyms: entactin 2, entactin-2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18074
VEGA: 14
Homologene: 40575
Tnni1
Name: troponin I, skeletal, slow 1
Synonyms: 2700018B22Rik, ssTnI
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 21952
Homologene: 2462
Dnah10
Name: dynein, axonemal, heavy chain 10
Synonyms: Dnahc10
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56087
HGNC: HGNC:2941
Homologene: 25816
Muc6
Name: mucin 6, gastric
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 353328
HGNC: HGNC:7517
Homologene: 18768
Nlrp9c
Name: NLR family, pyrin domain containing 9C
Synonyms: Nalp-zeta, Nalp9c
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330490
Homologene: 116072
Vmn1r50
Name: vomeronasal 1 receptor 50
Synonyms: VN2, V1rb1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 113852
Homologene: 113975
F12
Name: coagulation factor XII (Hageman factor)
Synonyms: FXII
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 58992
HGNC: HGNC:3530
Homologene: 425
Kdm1b
Name: lysine (K)-specific demethylase 1B
Synonyms: Aof1, 4632428N09Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218214
Homologene: 14695
Igsf10
Name: immunoglobulin superfamily, member 10
Synonyms: Adlican2, CMF608, 6530405F15Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242050
Homologene: 18712
Pde4dip
Name: phosphodiesterase 4D interacting protein (myomegalin)
Synonyms: D130016K21Rik, 9430063L05Rik, Usmg4, 4732458A06Rik, D3Bwg1078e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 83679
Homologene: 66961
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 435626
Homologene: 52359
Mtus2
Name: microtubule associated tumor suppressor candidate 2
Synonyms: 5730592G18Rik, A730013O20Rik, C130038G02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77521
Homologene: 78141
Ncf1
Name: neutrophil cytosolic factor 1
Synonyms: NOXO2, p47phox, Ncf-1, p47phox, NADPH oxidase subunit (47kDa)
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17969
Homologene: 30964
Or5b12
Name: olfactory receptor family 5 subfamily B member 12
Synonyms: GA_x6K02T2RE5P-3249780-3248836, MOR202-5, Olfr1448
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258696
Homologene: 64904
Ube2q1
Name: ubiquitin-conjugating enzyme E2Q family member 1
Synonyms: NICE-5, 2310012M18Rik, PRO3094, 1110002C01Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 70093
Homologene: 9730
Clip1
Name: CAP-GLY domain containing linker protein 1
Synonyms: cytoplasmic linker protein 50, restin, Clip 170, Clip50, Rsn, 4631429H07Rik, 1110007I12Rik, CLIP-170
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56430
Homologene: 74455
Pycr1
Name: pyrroline-5-carboxylate reductase 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 209027
HGNC: HGNC:9721
Homologene: 56002
Frs3
Name: fibroblast growth factor receptor substrate 3
Synonyms: Frs2beta, 4930417B13Rik, SNT2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 107971
Homologene: 4845
Cr2
Name: complement receptor 2
Synonyms: CD35, C3DR, Cr-1, Cr-2, Cr1, CD21
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12902
HGNC: HGNC:2336
Homologene: 55611
Piwil4
Name: piwi-like RNA-mediated gene silencing 4
Synonyms: Miwi2, MIWI2, 9230101H05Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330890
Homologene: 45122
Fhit
Name: fragile histidine triad gene
Synonyms: Fra14A2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 14198
HGNC: HGNC:3701
Homologene: 21661
Gpr149
Name: G protein-coupled receptor 149
Synonyms: Ieda, PGR10, R35, 9630018L10Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229357
Homologene: 16359
Chrng
Name: cholinergic receptor, nicotinic, gamma polypeptide
Synonyms: Acrg, Achr-3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11449
HGNC: HGNC:1967
Homologene: 3810
Celf3
Name: CUGBP, Elav-like family member 3
Synonyms: ERDA4, Tnrc4, BRUNOL1, CAGH4, 4930415M08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 78784
Homologene: 48510
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 74,147,663 bp
  • T to A, chromosome 1 at 87,206,639 bp
  • A to G, chromosome 1 at 135,805,053 bp
  • A to T, chromosome 1 at 195,155,888 bp
  • C to A, chromosome 3 at 59,336,514 bp
  • A to T, chromosome 3 at 62,595,128 bp
  • C to A, chromosome 3 at 87,973,122 bp
  • A to G, chromosome 3 at 88,693,136 bp
  • T to A, chromosome 3 at 89,761,591 bp
  • T to A, chromosome 3 at 89,781,076 bp
  • T to G, chromosome 3 at 94,488,538 bp
  • A to T, chromosome 3 at 97,841,797 bp
  • T to C, chromosome 3 at 108,337,909 bp
  • T to A, chromosome 3 at 108,668,113 bp
  • T to G, chromosome 4 at 42,937,879 bp
  • A to G, chromosome 5 at 123,631,078 bp
  • A to T, chromosome 5 at 124,747,630 bp
  • T to A, chromosome 5 at 134,226,609 bp
  • C to A, chromosome 5 at 148,295,506 bp
  • T to A, chromosome 6 at 90,107,494 bp
  • A to T, chromosome 7 at 26,371,451 bp
  • A to G, chromosome 7 at 28,767,671 bp
  • A to T, chromosome 7 at 113,313,545 bp
  • G to A, chromosome 7 at 141,638,400 bp
  • T to C, chromosome 9 at 14,736,956 bp
  • T to C, chromosome 11 at 120,642,246 bp
  • C to A, chromosome 13 at 19,102,959 bp
  • C to T, chromosome 13 at 47,063,077 bp
  • T to C, chromosome 13 at 55,418,137 bp
  • T to C, chromosome 14 at 9,870,095 bp
  • T to A, chromosome 14 at 19,763,711 bp
  • A to G, chromosome 14 at 30,008,772 bp
  • A to G, chromosome 14 at 31,715,260 bp
  • T to G, chromosome 17 at 47,703,636 bp
  • A to T, chromosome 19 at 12,919,667 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3548 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040667-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.