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Strain Name:
C57BL/6J-MtgxR3721Btlr/Mmmh
Stock Number:
040712-MU
Citation ID:
RRID:MMRRC_040712-MU
Other Names:
R3721 (G1), C57BL/6J-MtgxR3721Btlr
Major Collection:

Strain Information

Rab6b
Name: RAB6B, member RAS oncogene family
Synonyms: C330006L04Rik, D9Bwg0185e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270192
Homologene: 39749
Acvr2a
Name: activin receptor IIA
Synonyms: ActRIIa, tActRII, Acvr2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11480
HGNC: HGNC:173
Homologene: 20391
Ranbp17
Name: RAN binding protein 17
Synonyms: 4932704E15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66011
Homologene: 36409
Ash2l
Name: ASH2 like histone lysine methyltransferase complex subunit
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 23808
HGNC: HGNC:744
Homologene: 3436
Myh10
Name: myosin, heavy polypeptide 10, non-muscle
Synonyms: nonmuscle myosin heavy chain II-B, NMHC-B, SMemb, nonmuscle myosin heavy chain IIB, 9330167F11Rik, 5730504C04Rik, NMHC II-B, Myhn2, Myosin IIB, Myhn-2, myosin IIB, Fltn, Fltn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 77579
HGNC: HGNC:7568
Homologene: 55941
Naa25
Name: N(alpha)-acetyltransferase 25, NatB auxiliary subunit
Synonyms: 4833422K13Rik, C330023M02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231713
Homologene: 6891
Nol9
Name: nucleolar protein 9
Synonyms: 6030462G04Rik, 4632412I24Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74035
Homologene: 32589
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 20,585,655 bp
  • T to C, chromosome 1 at 21,260,089 bp
  • T to A, chromosome 1 at 40,537,088 bp
  • C to T, chromosome 1 at 82,290,085 bp
  • A to G, chromosome 1 at 89,145,328 bp
  • T to C, chromosome 1 at 143,695,453 bp
  • A to T, chromosome 1 at 161,063,308 bp
  • T to C, chromosome 2 at 48,892,138 bp
  • T to A, chromosome 3 at 28,727,152 bp
  • A to G, chromosome 3 at 59,097,670 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • C to T, chromosome 3 at 108,979,767 bp
  • A to G, chromosome 3 at 127,546,811 bp
  • T to A, chromosome 4 at 34,659,970 bp
  • T to A, chromosome 4 at 40,929,136 bp
  • G to A, chromosome 4 at 41,602,615 bp
  • T to C, chromosome 4 at 53,491,445 bp
  • T to G, chromosome 4 at 116,153,543 bp
  • T to A, chromosome 4 at 116,999,745 bp
  • T to C, chromosome 4 at 132,337,814 bp
  • T to G, chromosome 4 at 138,239,608 bp
  • A to G, chromosome 4 at 152,039,706 bp
  • T to A, chromosome 5 at 121,431,556 bp
  • T to A, chromosome 5 at 146,096,597 bp
  • G to A, chromosome 5 at 148,335,533 bp
  • C to A, chromosome 6 at 129,094,410 bp
  • A to C, chromosome 7 at 4,010,783 bp
  • A to G, chromosome 7 at 17,902,738 bp
  • C to A, chromosome 7 at 24,865,459 bp
  • A to G, chromosome 7 at 29,705,102 bp
  • T to A, chromosome 7 at 91,711,800 bp
  • G to T, chromosome 7 at 108,423,119 bp
  • T to C, chromosome 7 at 126,490,460 bp
  • T to C, chromosome 7 at 140,861,961 bp
  • C to G, chromosome 8 at 25,818,625 bp
  • A to G, chromosome 8 at 45,248,638 bp
  • C to T, chromosome 8 at 47,082,861 bp
  • G to A, chromosome 9 at 59,868,180 bp
  • T to C, chromosome 9 at 103,167,174 bp
  • T to C, chromosome 9 at 111,056,364 bp
  • A to G, chromosome 10 at 127,328,971 bp
  • GCCTGGATACTGACC to GCC, chromosome 11 at 33,219,203 bp
  • C to T, chromosome 11 at 50,773,211 bp
  • G to T, chromosome 11 at 68,813,052 bp
  • G to T, chromosome 11 at 80,196,917 bp
  • G to A, chromosome 11 at 84,521,828 bp
  • T to A, chromosome 13 at 52,508,023 bp
  • C to T, chromosome 14 at 50,186,680 bp
  • T to C, chromosome 15 at 10,488,026 bp
  • C to A, chromosome 15 at 55,642,818 bp
  • A to G, chromosome 15 at 82,103,204 bp
  • C to A, chromosome 15 at 99,772,663 bp
  • A to G, chromosome 16 at 5,073,378 bp
  • T to A, chromosome 17 at 8,969,589 bp
  • CTCCTCTTCTTCCTCTTCTTCCTC to CTCCTCTTCTTCCTC, chromosome 17 at 23,822,575 bp
  • T to C, chromosome 17 at 27,029,613 bp
  • T to C, chromosome 17 at 71,273,454 bp
  • A to G, chromosome 19 at 6,839,277 bp
  • T to C, chromosome 19 at 23,210,492 bp
  • A to G, chromosome 19 at 45,553,577 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3721 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040712-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.