Strain Name:
C57BL/6J-MtgxR3740Btlr/Mmmh
Stock Number:
040726-MU
Citation ID:
RRID:MMRRC_040726-MU
Other Names:
R3740 (G1), C57BL/6J-MtgxR3740Btlr
Major Collection:

Gene Information

Kif5a
Name: kinesin family member 5A
Synonyms: D10Bwg0738e, Kns, Khc, Kif5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 16572
VEGA: 10
HGNC: HGNC:6323
Homologene: 55861
Nup210l
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 77595
Homologene: 28122
Kat6b
Name: K(lysine) acetyltransferase 6B
Synonyms: Myst4, Morf, B130044K16Rik, monocytic leukemia, qkf, querkopf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 54169
Homologene: 136480
Dab1
Name: disabled 1
Synonyms: C630028C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 13131
HGNC: HGNC:2661
Homologene: 32084
Limk1
Name: LIM-domain containing, protein kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 16885
HGNC: HGNC:6613
Homologene: 1738
Zfp335
Name: zinc finger protein 335
Synonyms: 1810045J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 329559
Homologene: 11129
Fgfr1op2
Name: FGFR1 oncogene partner 2
Synonyms: 1500031J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 67529
Homologene: 9222
Farsb
Name: phenylalanyl-tRNA synthetase, beta subunit
Synonyms: Farslb, Frsb, PheRS alpha, Farsl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 23874
Homologene: 4160
Hacd3
Name: 3-hydroxyacyl-CoA dehydratase 3
Synonyms: Hspc121, Ptplad1, B-ind1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 57874
VEGA: 9
Homologene: 9494
Fam135a
Name: family with sequence similarity 135, member A
Synonyms: 4921533L14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 68187
Homologene: 32665
Optn
Name: optineurin
Synonyms: 4930441O07Rik, TFIIIA-INTP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 71648
Homologene: 11085
Mov10l1
Name: Mov10 like RISC complex RNA helicase 1
Synonyms: Csm, CHAMP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 83456
HGNC: HGNC:7201
Homologene: 56835
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Adgrb3
Name: adhesion G protein-coupled receptor B3
Synonyms: Bai3, A830096D10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 210933
HGNC: HGNC:945
Homologene: 1289
Zfp605
Name: zinc finger protein 605
Synonyms: A830023I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 675812
Homologene: 52335
Flt1
Name: FMS-like tyrosine kinase 1
Synonyms: vascular endothelial growth factor receptor-1, VEGFR-1, Flt-1, VEGFR1, sFlt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 14254
HGNC: HGNC:3763
Homologene: 134179
Serpinb6e
Name: serine (or cysteine) peptidase inhibitor, clade B, member 6e
Synonyms: ovalbumin, Gm11396, SPI3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 435350
HGNC: HGNC:8950
Homologene: 50933
Rims1
Name: regulating synaptic membrane exocytosis 1
Synonyms: RIM1alpha, RIM1, C030033M19Rik, RIM1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 116837
Homologene: 128399
Bbox1
Name: butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 170442
HGNC: HGNC:964
Homologene: 2967
Pik3cg
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma
Synonyms: PI3Kgamma, p110gamma, PI3Kgamma, 5830428L06Rik, PI(3)Kgamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 30955
HGNC: HGNC:8978
Homologene: 68269
Tiam2
Name: T cell lymphoma invasion and metastasis 2
Synonyms: STEF, 3000002F19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 24001
VEGA: 17
Homologene: 40796
Vmn2r107
Name: vomeronasal 2, receptor 107
Synonyms: V2r6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 22312
Homologene: 129750
Kcnk3
Name: potassium channel, subfamily K, member 3
Synonyms: Task-1, cTBAK-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 16527
HGNC: HGNC:6278
Homologene: 1692
Slc13a1
Name: solute carrier family 13 (sodium/sulfate symporters), member 1
Synonyms: NaSi-1, Nas1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 55961
Homologene: 31893
Ctso
Name: cathepsin O
Synonyms: A330105D01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229445
HGNC: HGNC:2542
Homologene: 1020
Mical1
Name: microtubule associated monooxygenase, calponin and LIM domain containing 1
Synonyms: Nical
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 171580
Homologene: 11246
Zfp69
Name: zinc finger protein 69
Synonyms: Zfp63, KRAB2, LOC381549
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 381549
Homologene: 45693
Vmn1r225
Name: vomeronasal 1 receptor 225
Synonyms: V1re5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 171228
Atp8b2
Name: ATPase, class I, type 8B, member 2
Synonyms: Id
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 54667
Homologene: 23226
Tmem88b
Name: transmembrane protein 88B
Synonyms: A230069A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 320587
Homologene: 85849
Syngr4
Name: synaptogyrin 4
Synonyms: 1700016O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 58867
Homologene: 8258
AC121888.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
Fam174b
Name: family with sequence similarity 174, member B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 100038347
Homologene: 121932
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 22,373,443 bp
  • A to T, chromosome 1 at 24,014,811 bp
  • T to C, chromosome 1 at 25,826,454 bp
  • T to A, chromosome 1 at 31,287,683 bp
  • A to G, chromosome 1 at 78,471,073 bp
  • C to A, chromosome 2 at 5,034,198 bp
  • T to A, chromosome 2 at 76,790,359 bp
  • A to G, chromosome 2 at 110,305,577 bp
  • G to A, chromosome 2 at 164,907,414 bp
  • G to A, chromosome 3 at 81,952,249 bp
  • C to G, chromosome 3 at 89,946,031 bp
  • A to T, chromosome 3 at 90,207,394 bp
  • C to T, chromosome 4 at 104,731,751 bp
  • T to A, chromosome 4 at 120,930,874 bp
  • A to G, chromosome 4 at 155,785,427 bp
  • T to C, chromosome 5 at 30,621,930 bp
  • G to T, chromosome 5 at 110,128,698 bp
  • A to C, chromosome 5 at 134,661,867 bp
  • G to A, chromosome 5 at 147,599,593 bp
  • T to C, chromosome 6 at 24,134,477 bp
  • A to G, chromosome 6 at 146,595,233 bp
  • T to C, chromosome 7 at 45,895,770 bp
  • T to C, chromosome 7 at 73,740,830 bp
  • T to C, chromosome 9 at 65,021,473 bp
  • A to C, chromosome 10 at 41,479,071 bp
  • A to G, chromosome 10 at 127,243,468 bp
  • T to C, chromosome 12 at 32,205,224 bp
  • T to A, chromosome 13 at 33,838,960 bp
  • T to G, chromosome 14 at 21,670,044 bp
  • A to G, chromosome 15 at 89,012,142 bp
  • A to G, chromosome 17 at 3,414,113 bp
  • A to G, chromosome 17 at 20,374,889 bp
  • A to G, chromosome 17 at 20,502,999 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3740 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040726-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.