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Strain Name:
C57BL/6J-MtgxR3755Btlr/Mmmh
Stock Number:
040738-MU
Citation ID:
RRID:MMRRC_040738-MU
Other Names:
R3755 (G1), C57BL/6J-MtgxR3755Btlr
Major Collection:

Strain Information

Ubap2
Name: ubiquitin-associated protein 2
Synonyms: 1190005K07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68926
Homologene: 73649
Lpp
Name: LIM domain containing preferred translocation partner in lipoma
Synonyms: B130055L10Rik, 9430020K16Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 210126
VEGA: 16
HGNC: HGNC:6679
Homologene: 4075
Nfib
Name: nuclear factor I/B
Synonyms: 6720429L07Rik, E030026I10Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18028
HGNC: HGNC:7785
Homologene: 4087
Chchd6
Name: coiled-coil-helix-coiled-coil-helix domain containing 6
Synonyms: 1700021B03Rik, 0710001P09Rik, Micos25
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66098
Homologene: 11920
Lrch4
Name: leucine-rich repeats and calponin homology (CH) domain containing 4
Synonyms: 2900069C24Rik, LRRN4, LRN, 2810008P14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231798
HGNC: HGNC:6691
Homologene: 20532
Knl1
Name: kinetochore scaffold 1
Synonyms: 2310043D08Rik, 5730505K17Rik, Casc5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 76464
Homologene: 44890
Etl4
Name: enhancer trap locus 4
Synonyms: Etl-4, E330027G05Rik, 6620402G01Rik, 9430077C05Rik, Skt, Sickle tail
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 208618
Homologene: 10477
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 162,544,220 bp
  • C to T, chromosome 1 at 171,777,160 bp
  • G to A, chromosome 2 at 20,743,537 bp
  • G to A, chromosome 2 at 82,978,217 bp
  • T to A, chromosome 2 at 119,102,579 bp
  • T to A, chromosome 2 at 120,586,245 bp
  • T to C, chromosome 2 at 167,974,223 bp
  • C to T, chromosome 3 at 75,105,671 bp
  • A to G, chromosome 3 at 92,825,039 bp
  • A to G, chromosome 3 at 154,764,321 bp
  • A to G, chromosome 4 at 41,195,482 bp
  • A to T, chromosome 4 at 82,323,699 bp
  • C to T, chromosome 4 at 116,993,404 bp
  • A to T, chromosome 4 at 119,367,808 bp
  • C to T, chromosome 4 at 130,312,387 bp
  • G to A, chromosome 5 at 8,747,403 bp
  • T to A, chromosome 5 at 137,637,730 bp
  • A to T, chromosome 6 at 37,364,026 bp
  • A to G, chromosome 6 at 83,288,303 bp
  • T to C, chromosome 6 at 89,574,355 bp
  • T to C, chromosome 6 at 112,693,471 bp
  • T to A, chromosome 6 at 136,560,817 bp
  • C to A, chromosome 7 at 5,017,885 bp
  • T to C, chromosome 7 at 45,408,945 bp
  • A to G, chromosome 7 at 105,330,151 bp
  • A to G, chromosome 8 at 84,893,480 bp
  • T to C, chromosome 8 at 93,528,502 bp
  • C to T, chromosome 8 at 109,632,539 bp
  • A to G, chromosome 9 at 64,289,795 bp
  • C to A, chromosome 10 at 29,222,114 bp
  • T to C, chromosome 10 at 53,625,952 bp
  • C to T, chromosome 10 at 81,144,266 bp
  • C to A, chromosome 10 at 127,659,507 bp
  • T to G, chromosome 11 at 96,852,103 bp
  • A to G, chromosome 11 at 102,399,259 bp
  • C to T, chromosome 13 at 104,168,560 bp
  • A to T, chromosome 14 at 68,577,138 bp
  • A to G, chromosome 15 at 9,367,412 bp
  • A to T, chromosome 15 at 44,589,406 bp
  • A to G, chromosome 15 at 83,131,475 bp
  • A to C, chromosome 15 at 98,378,582 bp
  • T to C, chromosome 15 at 99,306,945 bp
  • A to C, chromosome 16 at 16,763,020 bp
  • G to A, chromosome 16 at 17,898,963 bp
  • C to A, chromosome 16 at 20,689,183 bp
  • A to G, chromosome 16 at 24,845,161 bp
  • C to G, chromosome 16 at 97,436,657 bp
  • A to G, chromosome 17 at 21,229,009 bp
  • A to G, chromosome 17 at 32,359,132 bp
  • C to A, chromosome 17 at 33,932,597 bp
  • T to C, chromosome 17 at 58,104,599 bp
  • G to A, chromosome 17 at 73,331,119 bp
  • T to C, chromosome 18 at 34,372,474 bp
  • T to C, chromosome 18 at 34,808,296 bp
  • T to C, chromosome 18 at 37,302,825 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3755 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040738-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.