Strain Name:
C57BL/6J-MtgxR3797Btlr/Mmmh
Stock Number:
040758-MU
Citation ID:
RRID:MMRRC_040758-MU
Other Names:
R3797 (G1), C57BL/6J-MtgxR3797Btlr
Major Collection:

Gene Information

Gene Symbol: Itgb8 [MGI:1338035] (Mus musculus (mouse))
Name: integrin beta 8
Synonyms: 4832412O06Rik
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Limch1 [MGI:1924819] (Mus musculus (mouse))
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Lmf1 [MGI:1923733] (Mus musculus (mouse))
Name: lipase maturation factor 1
Synonyms: 2400010G15Rik, Tmem112
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Marc2 [MGI:1914497] (Mus musculus (mouse))
Name: mitochondrial amidoxime reducing component 2
Synonyms: 2810484M10Rik, Mosc2
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Zfp934 [MGI:1924367] (Mus musculus (mouse))
Name: zinc finger protein 934
Synonyms: 6720457D02Rik
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Xpnpep1 [MGI:2180003] (Mus musculus (mouse))
Name: X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
Synonyms: D230045I08Rik
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: B4galt1 [MGI:95705] (Mus musculus (mouse))
Name: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
Synonyms: beta 1,4-Galactosyltransferase I, Ggtb2, Ggtb, b1,4-Galactosyltransferase I, B-1,4-GalT1, beta-1,4-GalT1, GalT
Chromosome: 4
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 143,677,723 bp
  • T to C, chromosome 1 at 184,841,308 bp
  • C to A, chromosome 2 at 18,045,897 bp
  • T to C, chromosome 2 at 136,100,826 bp
  • G to A, chromosome 4 at 40,807,258 bp
  • G to C, chromosome 4 at 138,395,098 bp
  • T to C, chromosome 5 at 30,196,823 bp
  • A to G, chromosome 5 at 66,969,079 bp
  • C to T, chromosome 6 at 124,959,832 bp
  • G to A, chromosome 7 at 41,157,835 bp
  • C to A, chromosome 7 at 81,092,753 bp
  • T to C, chromosome 7 at 85,738,077 bp
  • G to A, chromosome 7 at 97,696,835 bp
  • C to T, chromosome 7 at 98,125,829 bp
  • C to T, chromosome 7 at 103,611,447 bp
  • C to T, chromosome 7 at 141,259,918 bp
  • G to A, chromosome 8 at 25,572,437 bp
  • T to C, chromosome 9 at 101,971,267 bp
  • T to C, chromosome 9 at 110,549,571 bp
  • G to A, chromosome 10 at 4,560,920 bp
  • A to G, chromosome 11 at 48,845,670 bp
  • A to G, chromosome 11 at 69,760,917 bp
  • G to A, chromosome 12 at 58,267,909 bp
  • T to C, chromosome 12 at 119,163,469 bp
  • T to A, chromosome 13 at 38,177,284 bp
  • T to C, chromosome 13 at 62,517,888 bp
  • A to T, chromosome 13 at 96,486,982 bp
  • A to T, chromosome 14 at 33,140,645 bp
  • G to A, chromosome 15 at 9,310,641 bp
  • C to A, chromosome 15 at 25,364,312 bp
  • A to G, chromosome 15 at 77,968,569 bp
  • T to C, chromosome 16 at 5,104,550 bp
  • G to A, chromosome 17 at 25,654,471 bp
  • A to G, chromosome 17 at 26,068,526 bp
  • C to T, chromosome 17 at 35,439,438 bp
  • T to C, chromosome 17 at 73,907,658 bp
  • A to G, chromosome 17 at 75,362,630 bp
  • T to A, chromosome 18 at 5,060,534 bp
  • T to C, chromosome 19 at 5,167,912 bp
  • T to C, chromosome 19 at 16,745,947 bp
  • A to T, chromosome 19 at 53,006,342 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3797 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040758-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.